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New: TruSeq® Stranded mRNA
Today Eppendorf announces the availability of the epMotion automation method for Illumina’s TruSeq Stranded mRNA library preparation kit for next-generation sequencing as “Illumina Qualified.” “Illumina Qualified” indicates that Illumina’s analysis of libraries prepared with this epMotion method has shown the libraries to perform comparably to those prepared manually.
For next generation sequencing systems a variety of sample preparation kits are available. These kits are needed to convert either DNA or RNA samples into sequencing ready libraries, a procedure that includes many steps and can be time consuming. RNA sequencing requires additional steps – either the depletion of unwanted ribosomal or the positive selection of mRNA from total RNA samples. Due to the complexity of the library construction methods, automation is regarded as highly useful.
The epMotion method can be used for the automated construction of 8, 16 or 24 libraries starting with 100 – 1000 ng of total RNA together with Illumina’s TruSeq Stranded mRNA kit. The overall hands-on time is less than 1.5 hours, while the total run time of the entire procedure is ~11.5 hours for 24 samples. Minimized hands-on time & high reproducibility with this automated method help to streamline and standardize the sequencing workflow.
Additional automated methods for NGS library preparation from Illumina are currently in development.
New epMotion 96
The new epMotion 96 from Eppendorf provides an ideal, time-saving solution for scientists undertaking the repetitive task of working with 96 or 384 format microplates or deep-well plates within life science research laboratories and pharmaceutical and industry laboratories. The epMotion 96 allows easy, rapid handling with high precision and accuracy compared to multi-channel manual pipettes and thanks to its ability to dispense into all 96 wells at the same time, makes it possible to simultaneously start or stop a biological assay for all wells in a plate. The system also makes the task more comfortable and efficient for laboratory workers by greatly reducing the risk of repetitive stress injury caused by manual pipetting.
Nueva promoción Eppendorf Advantage™
¡Todo al cinco! Grandes solucionespara el sistema de 5 mL
La nueva promoción incluye dos packs de iniciofáciles y completas para el manejo de muestras con volúmenes medios enEppendorf Tubes® 5.0 mL. Ahorre hasta un 25 %. Benefíecese además de un ahorrohasta un 15 % comprando uno de los nuevos New Capacity Packs con centrífugas5430, 5430 R o 5427 R. Estos packs incluyen una centrífuga, dos rotores, tubosy racks de 5,0 mL.
Más información en www.eppendorf.es/advantage
Hot off the press: Eppendorf BioNews No. 41
It’s time to experience a new dimension in cell culture!
The leading article of Eppendorf BioNews No. 41 features the new Eppendorf Cell Culture Consumables. You can also learn more about the DASGIP® product line for cell culture applications. It combines the advantages of small working volumes with the full functionality of industrial bioreactors. Further products featured in this issue include the Mastercycler® nexus X2, systems for micromanipulation of cells, Eppendorf Serological Pipets and lots more. Additionally readers will find detailed Application Notes and the popular competition with great prizes.
Madeline Lancaster wins Eppendorf Award 2014
The American scientist Madeline Lancaster, Ph. D. (Marie Curie Postdoctoral Research Fellow, Laboratory of Juergen Knoblich at the Institute of Molecular Biotechnology of the Austrian Academy of Sciences, IMBA, Vienna, Austria) has won the 2014 Eppendorf Award for Young European Investigators.
Madeline Lancaster, born 1982, received the Eppendorf Award for her work showing that complex neuronal tissues resembling early states of fetal human brain can be created in vitro from pluripotent stem cells. Her groundbreaking experiments allow for the first time to recapitulate the development of brain structures in a three-dimensional organoid. Madeline Lancaster also documented that diseases caused by aberrant development such as microcephaly can be reproduced in the organoid culture. These discoveries allow for novel approaches towards the understanding of neurological diseases.
The Award ceremony took place at the EMBL Advanced Training Centre in Heidelberg, Germany, on 22 May 2014.
More information at www.eppendorf.com/award
Eppendorf BioSpectrometer® fluorescence
The new BioSpectrometer fluorescence combines UV/Vis absorption measurements with fluorescence options in one device. The fluorescence unit of the BioSpectrometer fluorescence increases the measuring range by a factor of 1,000, for example, to detect dsDNA. Just use the integrated fluorescence unit. As an accessory the Eppendorf µCuvette G1.0 can be used to measure higher concentrated samples. Thus, the system solution of BioSpectrometer fluorescence and µCuvette G1.0 covers a dynamic measuring range of more than 6 logs, from 0.001 to 1,500 ng/µL dsDNA.
> UV/Vis spectrophotometer to execute measurements from 200 nm to 830 nm
> Perform fluorescence measurements in the same device
> Sensitive quantification down to a concentration of 0.001 ng/µL dsDNA
> Eppendorf µCuvette® G1.0 as optional accessory for measuring > 1.5 µL sample volumes
> The system solution (BioSpectrometer fluorescence & µCuvette G1.0) offers an extremely broad measuring range (0.001 – 1,500 ng/ µL)
The working principle of the BioSpectrometer fluorescence and the arrangement of the optical paths are illustrated in the following animation:
Find out more: www.eppendorf.com/photometry
Impress Yourself: The new Eppendorf Cell Culture Consumables
Experience a new dimension in cell culture work. In the development of the new Eppendorf Cell Culture Consumables we put a special focus on your needs for excellent cell performance and reliability as well as for advanced protection against contamination. The outstanding design, reliability and purity is based on more than 50 years of experience. Products created by experts, developed for perfectionists.
Impress Yourself: Nuevos Consumibles de Cultivo Celular de Eppendorf
En el desarrollo de nuestro amplio catálogo de consumibles para cultivo celular hemos puesto especial énfasis en sus necesidades. Un excelente rendimiento y una excelente fiabilidad, así como una protección avanzada contra la contaminación, son nuestros objetivos principales. Nuestro diseño excepcional, la fiabilidad y la pureza son el resultado de más de 50 años de experiencia en productos desarrollados por expertos y perfeccionistas.
New Eppendorf epMotion® 5070 & 5075 automated pipetting systems
Eppendorf has launched seven new models to its successful epMotion range of automated pipetting systems. Designed for effective, automated liquid handling applications, the new epMotion 5070 and epMotion 5075 systems provide a precise, accurate alternative to time-consuming manual pipetting and liquid handling tasks. The combination of accelerated operational speed, new software & hardware features and an increased number of worktable positions make the systems ideal for simple and flexible laboratory use across a wide range of liquid handling applications. The new Eppendorf EasyCon™ and Eppendorf MultiCon™ controllers work with touch, mouse or keyboard for very easy and convenient handling of the epMotion systems with an improved version of the well-established epBlue™ software. To ensure simple programming of applications, even for users with only initial training, a new concept of user guided software assistants is provided. Step-by-step the user is guided through the programming of applications like PCR setup, NA preparation or general liquid handling tasks. The five larger epMotion 5075 models can be ordered with the CleanCap option, with UV lamp for decontamination and HEPA filter for clean air inside the automation workstation. The smaller epMotion 5070 & 5070f are now available with automatic tool exchange and re-use tip capability and are our most compact solution for accurate and reproducible pipetting. The new epMotion 5075m is the most flexible member of the epMotion family of automated pipetting systems. With the large number of deck positions and the mixing, temperature control and magnetic bead separation abilities it is ideally suited for various applications in the lab.
Discover the variety of automated liquid handling with the new Eppendorf epMotion series: www.eppendorf.com/automation