Eppendorf licenses technology for thermal cyclers to SensoQuest
Eppendorf AG has licensed patents covering gradient technology for thermal cyclers to SensoQuest GmbH, including German patent Nos. DE 196 55 282 and DE 196 55 141. Eppendorf utilizes the technology in its Eppendorf Mastercycler® pro gradient and Mastercycler nexus gradient models.
New: TruSeq® Stranded mRNA
Today Eppendorf announces the availability of the epMotion automation method for Illumina’s TruSeq Stranded mRNA library preparation kit for next-generation sequencing as “Illumina Qualified.” “Illumina Qualified” indicates that Illumina’s analysis of libraries prepared with this epMotion method has shown the libraries to perform comparably to those prepared manually.
For next generation sequencing systems a variety of sample preparation kits are available. These kits are needed to convert either DNA or RNA samples into sequencing ready libraries, a procedure that includes many steps and can be time consuming. RNA sequencing requires additional steps – either the depletion of unwanted ribosomal or the positive selection of mRNA from total RNA samples. Due to the complexity of the library construction methods, automation is regarded as highly useful.
The epMotion method can be used for the automated construction of 8, 16 or 24 libraries starting with 100 – 1000 ng of total RNA together with Illumina’s TruSeq Stranded mRNA kit. The overall hands-on time is less than 1.5 hours, while the total run time of the entire procedure is ~11.5 hours for 24 samples. Minimized hands-on time & high reproducibility with this automated method help to streamline and standardize the sequencing workflow.
Additional automated methods for NGS library preparation from Illumina are currently in development.
New epMotion 96
The new epMotion 96 from Eppendorf provides an ideal, time-saving solution for scientists undertaking the repetitive task of working with 96 or 384 format microplates or deep-well plates within life science research laboratories and pharmaceutical and industry laboratories. The epMotion 96 allows easy, rapid handling with high precision and accuracy compared to multi-channel manual pipettes and thanks to its ability to dispense into all 96 wells at the same time, makes it possible to simultaneously start or stop a biological assay for all wells in a plate. The system also makes the task more comfortable and efficient for laboratory workers by greatly reducing the risk of repetitive stress injury caused by manual pipetting.
Hot off the press: Eppendorf BioNews No. 41
It’s time to experience a new dimension in cell culture!
The leading article of Eppendorf BioNews No. 41 features the new Eppendorf Cell Culture Consumables. You can also learn more about the DASGIP® product line for cell culture applications. It combines the advantages of small working volumes with the full functionality of industrial bioreactors. Further products featured in this issue include the Mastercycler® nexus X2, systems for micromanipulation of cells, Eppendorf Serological Pipets and lots more. Additionally readers will find detailed Application Notes and the popular competition with great prizes.
Have an old Eppendorf microcentrifuge? Win a new Centrifuge 5427 R
We're on a mission to track down some of the oldest Eppendorf microcentrifuges in laboratories across the UK and Ireland.
If you have an old Eppendorf microcentrifuge, which may likely resemble a model featured in the picture opposite, then you have a good chance of winning a brand new Eppendorf Centrifuge 5427 R.
Simply upload a picture of your old microcentrifuge to our Facebook page stating it's serial no. In return we'll send you a free copy of our Eppendorf Centrifugation Guide providing an insight into centrifuge safety, maintenance, troubleshooting and useful tips and tricks (name and address required).
*Competition closes 31st December 2014. The winner will be announced on our Facebook page by 14th January 2015.
Eppendorf UK launches new website
Eppendorf has launched a new, updated version of its UK website, combining its existing eShop with a comprehensive library of product information. Customers can now access everything they need to make informed purchase decisions on the complete range of Eppendorf premium laboratory products. Visitors to the site can order free consumables samples, take advantage of current promotional offers, and access valuable service and customer support such as the Eppendorf epServices offering – all from within a single, easy to navigate location.
The website contains all Eppendorf product lines including an extensive array of bioprocess products. With an all-inclusive set of technical information, including FAQ’s, operating manuals, catalogues and brochures, application notes and material data safety sheets, customers have easy access to all the information they require to ensure their equipment fulfils their precise needs. Users can start shopping without having to log in, or can choose to register to the myEppendorf portal, which gives access to online product registration and the epPoints loyalty program.
“With the Eppendorf training centre, application support and information on local distributor partners, as well as comprehensive product information, delivered across in a clear and efficient site, it has never been easier to equip your laboratory with the tools to optimise your research, no matter your specialty,” explains Edwin van Weert, Managing Director of Eppendorf UK Ltd.
Visit the updated Eppendorf website at www.eppendorf.co.uk
Madeline Lancaster wins Eppendorf Award 2014
The American scientist Madeline Lancaster, Ph. D. (Marie Curie Postdoctoral Research Fellow, Laboratory of Juergen Knoblich at the Institute of Molecular Biotechnology of the Austrian Academy of Sciences, IMBA, Vienna, Austria) has won the 2014 Eppendorf Award for Young European Investigators.
Madeline Lancaster, born 1982, received the Eppendorf Award for her work showing that complex neuronal tissues resembling early states of fetal human brain can be created in vitro from pluripotent stem cells. Her groundbreaking experiments allow for the first time to recapitulate the development of brain structures in a three-dimensional organoid. Madeline Lancaster also documented that diseases caused by aberrant development such as microcephaly can be reproduced in the organoid culture. These discoveries allow for novel approaches towards the understanding of neurological diseases.
The Award ceremony took place at the EMBL Advanced Training Centre in Heidelberg, Germany, on 22 May 2014.
More information at www.eppendorf.com/award
Eppendorf BioSpectrometer® fluorescence
The new BioSpectrometer fluorescence combines UV/Vis absorption measurements with fluorescence options in one device. The fluorescence unit of the BioSpectrometer fluorescence increases the measuring range by a factor of 1,000, for example, to detect dsDNA. Just use the integrated fluorescence unit. As an accessory the Eppendorf µCuvette G1.0 can be used to measure higher concentrated samples. Thus, the system solution of BioSpectrometer fluorescence and µCuvette G1.0 covers a dynamic measuring range of more than 6 logs, from 0.001 to 1,500 ng/µL dsDNA.
> UV/Vis spectrophotometer to execute measurements from 200 nm to 830 nm
> Perform fluorescence measurements in the same device
> Sensitive quantification down to a concentration of 0.001 ng/µL dsDNA
> Eppendorf µCuvette® G1.0 as optional accessory for measuring > 1.5 µL sample volumes
> The system solution (BioSpectrometer fluorescence & µCuvette G1.0) offers an extremely broad measuring range (0.001 – 1,500 ng/ µL)
The working principle of the BioSpectrometer fluorescence and the arrangement of the optical paths are illustrated in the following animation:
Find out more: www.eppendorf.com/photometry
Impress Yourself: The new Eppendorf Cell Culture Consumables
Experience a new dimension in cell culture work. In the development of the new Eppendorf Cell Culture Consumables we put a special focus on your needs for excellent cell performance and reliability as well as for advanced protection against contamination. The outstanding design, reliability and purity is based on more than 50 years of experience. Products created by experts, developed for perfectionists.
Society of Biology names Eppendorf as official sponsor of its amateur photography competition
The annual amateur photography competition run by the Society of Biology recognises some of the most inspiring images of the natural world. The theme for the 2014 competition is ‘Home, Habitat and Shelter’ and opened on the 10th March to submissions showing the unique ways animals, plants and organisms exploit their environment to survive. Eppendorf, has proudly been named as the official sponsor of this year’s event.
Entries can be submitted for the adult and under-18 categories until the 31st July, with the winners to be announced in October during Biology Week 2014.
The Society is holding a free exhibition of shortlisted entries from the past two years to coincide with the launch of this year’s competition. These are being shown in the atrium of the Royal Institution until the 11th April and will also feature 2013’s winning image, ‘Hunting Nectar’ (pictured right) by Putu Sudiarta.
More information on The Society of Biology’s Amateur photography competition