BioPhotometer® D30
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Is it possible to use the Eppendorf µCuvette also in the BioPhotometer D30?

What is the minimum volume for nucleic acid solution that I can use in the Eppendorf µCuvette that I can use in the BioPhotometer D30?

What is the minimum volume for protein solution that I can use in the Eppendorf µCuvette that I can use in the BioPhotometer D30?

Is it possible to carry out a wavelength scan with the BioPhotometer D30?

What wavelength could be applied in the BioPhotometer D30?

What methods are preprogrammed in the BioPhotometer D30?

Is it possible to use the BioPhotometer Data Transfer Software for the BioPhotometer D30?

What can I do with the so called "impurity scan"?

For what methods the "impurity scan" could be applied?

What wavelength range is used for the "impurity scan"?

Is it possible to change the range for the "impurity scan" and can I display the single absorbance values of the "impurity scan"?

Can I use the Hellma® TrayCell with the BioPhotometer D30?

What kind of cuvette path lengths could be applied for the BioPhotometer D30?

Is there any possibility to check the BioPhotometer D30?

In what file-format are the files from the BioPhotometer D30 exported?

Is it possible to select my data for the export?

What kind of SI-units can be applied with the BioPhotometer D30?

In the BioPhotometer D30 certain factors are preprogrammed for the evaluation of specific proteins. Is it possible to apply factors also for other proteins?

What is the meaning of the value "A0,1%"?

How many methods can be stored in the BioPhotometer D30?

How many results can be stored in the BioPhotometer D30?

Is it possible to use the Eppendorf µCuvette also in the BioPhotometer D30?Back to top
Yes, this is possible without any restrictions
What is the minimum volume for nucleic acid solution that I can use in the Eppendorf µCuvette that I can use in the BioPhotometer D30?Back to top
The smallest volume should be at least 1.5 µL.
What is the minimum volume for protein solution that I can use in the Eppendorf µCuvette that I can use in the BioPhotometer D30?Back to top
This depends also to some extend to on the proteins. We recommend a volume of at least 3 µL.
Is it possible to carry out a wavelength scan with the BioPhotometer D30?Back to top
No, this is unfortunately not possible. The BioPhotometer D30 is a fix wavelength photometer for specific methods.
What wavelength could be applied in the BioPhotometer D30?Back to top
The wavelengths 230, 260, 280, 320, 340, 405, 490, 562, 595 und 600 nm could be applied.
What methods are preprogrammed in the BioPhotometer D30?Back to top
Preprogrammed are all nucleic acid and protein detection methods and the OD 600 method for the determination of the optical density of bacterial or yeast cultures. Protein can be detected at 280 nm and via colorimetric assays like the BCA, the Bradford or the Lowry method.
Is it possible to use the BioPhotometer Data Transfer Software for the BioPhotometer D30?Back to top
No, this is unfortunately not possible. But this is also not necessary since the results are exported directly as Excel-files. An additional program for data management is therefore not necessary.
What can I do with the so called "impurity scan"?Back to top
With the "impurity scan" the absorbance of a certain wavelength range is shown for nucleic acid and protein measurements. With this function impurities in the sample can be easily detected via deviations from the known curve.
For what methods the "impurity scan" could be applied?Back to top
This function is available for nucleic acid and protein detections.
What wavelength range is used for the "impurity scan"?Back to top
For protein measurements it is the wavelength range from 245-350 nm and for nucleic acids the range from 225-350nm.
Is it possible to change the range for the "impurity scan" and can I display the single absorbance values of the "impurity scan"?Back to top
No, unfortunately both options are not possible. This can be done only in the BioSpectrometer
Can I use the Hellma® TrayCell with the BioPhotometer D30?Back to top
Yes, this is of course possible! Please check, that the cuvette with the required light beam hight of 8.5 mm is applied. Furthermore it is necessary for the precise calculation of the sample concentration that the correct light path length is set in the parameter section (stated on the lid)
What kind of cuvette path lengths could be applied for the BioPhotometer D30?Back to top
Like for the BioSpectrometer the cuvette path lengths of 10, 5, 2, 1, 0.2, 0.1 mm could be programmed.
Is there any possibility to check the BioPhotometer D30?Back to top
Yes, this is possible. We offer a calibration filter set especially for the BioPhotometer D30.
In what file-format are the files from the BioPhotometer D30 exported?Back to top
The data will be exported as Excel-files
Is it possible to select my data for the export?Back to top
Yes, this is possible! In the method you can select in the section "print&export" specific data packages and specific results for the export.
What kind of SI-units can be applied with the BioPhotometer D30?Back to top
This depends on the method. The free programmable methods offer 18 different kind of SI units. But it is possible at any time to edit new SI-Units?
In the BioPhotometer D30 certain factors are preprogrammed for the evaluation of specific proteins. Is it possible to apply factors also for other proteins?Back to top
Yes, this is possible. Select in the "parameter" section of the method "protein". With "edit" a calculation tools for protein factor starts. Here, you may set the factor for the new protein directly. It is also possible to calculate the factor via the absorbance coefficient and the molecular mass of the protein or via its value "A0.1%"
What is the meaning of the value "A0,1%"?Back to top
The meaning of A0,1% is the absorbance of the Protein with a concentration of 1mg/ml.
How many methods can be stored in the BioPhotometer D30?Back to top
About 100 methods can be stored in the BioPhotometer D30 or the BioSpectrometer.
How many results can be stored in the BioPhotometer D30?Back to top
About 1000 results can be stored in the BioPhotometer D30 or the BioSpectrometer.
 
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