Frequently Asked Questions
Select the product group below to get answers to common product questions. If you need additional help, our Scientific Support group is ready to assist you.
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Yes, this is possible without any restrictions
The smallest volume should be at least 1.5 µL.
This depends also to some extend to on the proteins. We recommend a volume of at least 3 µL.
No, this is unfortunately not possible. The BioPhotometer D30 is a fix wavelength photometer for specific methods.
The wavelengths 230, 260, 280, 320, 340, 405, 490, 562, 595 und 600 nm could be applied.
Preprogrammed are all nucleic acid and protein detection methods and the OD 600 method for the determination of the optical density of bacterial or yeast cultures. Protein can be detected at 280 nm and via colorimetric assays like the BCA, the Bradford or the Lowry method.
No, this is unfortunately not possible. But this is also not necessary since the results are exported directly as Excel-files. An additional program for data management is therefore not necessary.
With the "impurity scan" the absorbance of a certain wavelength range is shown for nucleic acid and protein measurements. With this function impurities in the sample can be easily detected via deviations from the known curve.
This function is available for nucleic acid and protein detections.
For protein measurements it is the wavelength range from 245-350 nm and for nucleic acids the range from 225-350nm.
No, unfortunately both options are not possible. This can be done only in the BioSpectrometer
Yes, this is of course possible! Please check, that the cuvette with the required light beam hight of 8.5 mm is applied. Furthermore it is necessary for the precise calculation of the sample concentration that the correct light path length is set in the parameter section (stated on the lid)
Like for the BioSpectrometer the cuvette path lengths of 10, 5, 2, 1, 0.2, 0.1 mm could be programmed.
Yes, this is possible. We offer a calibration filter set especially for the BioPhotometer D30.
The data will be exported as Excel-files
Yes, this is possible! In the method you can select in the section "print&export" specific data packages and specific results for the export.
This depends on the method. The free programmable methods offer 18 different kind of SI units. But it is possible at any time to edit new SI-Units?
Yes, this is possible. Select in the "parameter" section of the method "protein". With "edit" a calculation tools for protein factor starts. Here, you may set the factor for the new protein directly. It is also possible to calculate the factor via the absorbance coefficient and the molecular mass of the protein or via its value "A0.1%"
The meaning of A0,1% is the absorbance of the Protein with a concentration of 1mg/ml.
About 100 methods can be stored in the BioPhotometer D30 or the BioSpectrometer.