Microinjection techniques are widely applied in developmental biology for the analysis of early developmental processes such as gastrulation, neural induction and patterning or organogenesis. Microinjection experiments into vertebrate embryos (e.g. mouse, frog, fish) allow to generate transgenic animals by injection of DNA, to interfere with specific developmental processes (DNA, RNA, morpholino-injection), or to follow the fate of individual cells by the injection of fluorescent lineage tracer dyes.