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Frequently Asked Questions
Select the product group below to get answers to common product questions. If you need additional help, our Scientific Support group is ready to assist you.
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I work with the Bradford protein determination method. During my latest sample measurement on the BioPhotometer the measured absorbance appeared on the display, but a concentration wasn't displayed. What causes this?
I would like to carry out a colorimetric detection on the BioPhotometer with several standards and using linear regression. Following the measurement, can I query the factor or the ascent of the calibration lines on the BioPhotometer?
In your "Tips & Tricks" list for the photometric determination of nucleic acid concentrations you say under "Cuvette handling" that for very exact measurements a cuvette error should be determined and allowed for in the measured results. How do I determine the cuvette error?
In the course of my DNA concentration determination I measured a relatively low A260 value (between 0.05 and 0.07) and a relatively high A230 value (corresponding app. to the A260 values). The A260/A230 is <2.0. What do these values say about the quality of my DNA?
These tips will grant an advantage especially during pipetting of solutions containing detergents, such as PCR master mixes, enzyme solutions and many buffers. The LoRetention tips are characterized by significantly improved flow performance, thus achieving more economic use of expensive reagents, while valuable sample material is used effectively. Furthermore, reproducibility is improved, since wetting of the tip is dependent on both user and handling (for example: pipetting speed) and can therefore vary considerably.
No, the tips are uncoated. The ultra-hydrophobic surface is achievd through an innovative treatment at the molecular level.
The LoRetention tips are made from polypropylene, as are standard tips, and they are resistant to a number of organic solvents. Further information regarding chemical resistance properties of pipette tips can be found in the User Guide no. 23: Chemical Stability of Consumables.
The lids of the LoRetention racks and reloads have a transparent lid as compared to the blue lid of the standard tips. A reusable LoRetention adhesive sticker is placed on the lid. This sticker also features “LoRetention” writing on the reverse side. This way previously purchased epT.I.P.S. boxes can be labeled. When the box is open, the writing “LoRetention” will thus be easily readable.
Yes. Foam formation is rarely observed with the use of LoRetention tips. This facilitates the handling of these solutions and increases reproducibility.
Yes, the batch-specific purity grades can be downloaded at www.eppendorf.com/certificates.
Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.
All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.
The previous purity grades were developed due to customer requirements and new applications, in the course of the years. To meet the customer requirements for single applications even better, Eppendorf has extended the certification of the purity grades "Sterile" (now, also certified as free of pyrogenes) and "Biopur" (now, also certified as free of DNase and PCR inhibitors). In addition, the previous purity grades have been transferred to a new modular system that provides the same variety and flexibility and yet is readily understandable.
No, they are the same products as before, i.e. all specifications as dimensions remain the same. Only the packing has changed, due to the new seals. In addition, the certification of "Sterile" and "Biopur" has been extended but the entire production process was maintained.
For all purity grades, there will be new packing seals. In addition, there will be a further certification for the purity grades "Sterile" and "Biopur". "Sterile" will certify sterility and also the freedom of pyrogenes. The purity grade "Biopur" also includes the freedom of DNase as well as of PCR inhibitors.
Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.
Plates made from polypropylene may be used across a broad spectrum of applications, as they are more resistant to temperature variations as well as various chemicals than plates made from polystyrene. Furthermore, they are more robust, allowing them to be centrifuged at higher g-forces. In addition, biomolecules, such as nucleic acids and proteins, bind less strongly to polypropylene.
The Eppendorf PP Microplates are made from polypropylene and are thus resistant to DMSO and other organic solvents, unlike plates made from polystyrene. An overview of chemical resistance can be found in Application Note no. 56. In case of doubt please contact Eppendorf Application Support: firstname.lastname@example.org.
All areas of the plate, including the colored rim, are resistant to chemicals. The alpha numeric labeling is equally durable. An overview of chemical resistance can be found in Application Note no. 56. In case of doubt please contact Eppendorf Application Support: email@example.com.
Eppendorf offers a variety of sealing options for Microplates. Self-adhesive sealers, films and foils for heat sealing, as well as re-usable sealing mats, are available. Information regarding the compatibility of the different sealing methods is listed in the Application Note no. 208.
Plates made from black material are especially well suited for fluorescence assays (e.g. PicoGreen®). The black wells prevent “cross talk” between wells and reduce background signals. The black Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in black plates. Due to carefully selected materials, the plates have very low autofluorescence. The superior signal-to-noise ratio contributes to the increased sensitivity of fluorescence assays (see Application Note no. 203).
Plates made from white material may be used for luminescence assays (chemiluminescence, scintillation proximity assays (SPA) etc.), as well as for fluorescence assays (fluorescence intensity, polarization, etc.). The white wells prevent “cross talk” between the wells while amplifying the individual signals. The white Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in white plates. Due to carefully selected materials, the plates offer maximum signal amplification. Due to their very low autofluorescence, the white Eppendorf Microplates are equally well suited for fluorescence assays. Taken together, these features allow for an improvement in the sensitivity of analyses.
No, we recommend storage at a minimum of –80°C.
Yes; however, the exact specifications of the Microplates could be compromised. The plates are available in the qualities sterile or PCR clean. Hence, autoclaving is necessary only under exceptional circumstances.
No, the LoBind quality of the Eppendorf PP Microplates is not derived from siliconization or any other type of coating. The functionality is achieved through a carefully selected material, in combination with a special manufacturing process.
The Eppendorf PP Microplates may be centrifuged up to 6000 x g. Please follow the instructions provided in the manual.
Plates made from polypropylene are not suited for the standard variant, where antigen or antibodies are bound to the well wall. For this application, plates made from polystyrene are used, which have a higher binding capacity for biomolecules. However, for antibody-based fluorescence or luminescence assays (e.g. with beads) Eppendorf PP Microplates with black or white wells are suitable, respectively.
Typically, absorption measurements are not performed in plates made from polypropylene, but in plates made from polystyrene, as this material generally has a very high transparency. Since the Eppendorf PP Microplates show considerably higher transparency compared to other plates made from polypropylene (see Application Note no. 202), the Eppendorf Microplates may be used for absorption measurements in certain cases.
The Eppendorf PP Microplates are a very high quality product. In order to underline the quality, all plates are available in the degrees of purity PCR clean or Sterile. This increases the confidence and comfort of the user, who may employ the plates directly for valuable samples in sophisticated applications.
Yes, the option of Eppendorf Microplates with barcode is available. Further information can be found on the product page www.eppendorf.com/microplates.
At least 5 years at a storage temperature of +5 to +35 °C.
Lead, cadmium, mercury, etc. are well below the prescribed limits of the DIN norm. In the course of the production process it is impossible to avoid the smallest quantities of metal resulting from machine friction.
Yes. We certify our PC plates and sealing options with "PCR-Clean". This certificate stands for guaranteed freedom from human DNA, DNase, RNase and PCR inhibitors.
"The lower part of the Xplorer can be autoclaved. 175 autoclaving cycles (121 °C, 1 bar, 20 min.) were tested. The number of the corresponding tests corresponds to an average 5-year use of the pipette."
"Yes, the Xplorer is resistant to solvents. Only the display cannot come into contact with the solvent."
"Yes, acids can be dispensed with the Xplorer. But their higher density must be taken into consideration and the Xplorer should be adjusted to the changed physical data in the ""Options"" operating mode."
"Yes, there are several options for adjusting the pipette in the Options operating mode, e.g. to ethanol 75 %25 or gylcerol 50 %25. Furthermore, individual adjustments are possible, as 1-point, 2-point or 3-point settings."
In the "Pipetting and mixing" operating mode, the mixing volume can be defined independent from the filling volume.
Yes, the volume of the acustic feedback can be set to the levels 0-8 in the Options operating mode.
"A complete run with volume, speed, etc. can be saved at the program level. After exiting the program level, the run can no longer be changed just by pressing the keys. "
This is independent from the aspiration volume. Please contact the Application Hotline at firstname.lastname@example.org if you have any further questions.
Based on a speed level between min. 2.6 S (Step 1) and max. 12 S (Step 8).
"The Xplorer is UV-stable. Longer periods of irradiation can lead to very minimal material discoloration that does not have any effect on functionality."
"The Xplorer technical data is only valid for pipetting. Conformity must be achieved for this operating mode. For piston-stroke pipettes, there are no requirements for dispensing accuracy according to EN ISO 8655."
Yes, a volume limit can be defined in the "Options" operating mode.
"The Xplorer can be charged using the power supply included in the accessories or the optionally available charging stand or charging carousel. One Xplorer can be charged with the charging stand and four with the charging carousel. A single-channel or multi-channel Xplorer can be attached to a charging shell."
"No, the Xplorer cannot be charged in the Multipette charging stand. But the charging shells of the Xplorer charging stand can be exchanged. A charging shell for a Multipette stream/Xstream can be used instead of a charging shell for an Xplorer. "
The li-ion battery features high energy density. Its usable service life is several years; but this is strongly dependent on usage and storage conditions.
Yes the battery can be individually ordered by customers as a spare part, or reordered.
Due to the reduction in dispensing speed and additional vacuums of the Blow out, the sample can be accurately dispensed in the gel-slots. The enormous synchronization of the Xplorer motor also achieves very good results. In the operating mode "Pipetting and mixing P/M," the sample is automatically mixed with the preparation after dispensing. The mixing cycles and mixing volume can be individually defined for this.
In the operating mode "Pipetting and mixing P/M," the sample is automatically mixed with the preparation after dispensing. The mixing cycles and mixing volume can be individually defined for this.
In the operating mode "Pipetting and mixing P/M," the sample is automatically mixed with the preparation after dispensing. The mixing cycles and mixing volume can be individually defined for this.
On the Xplorer, the pipette can be adjusted to different liquids or geographic heights using 1, 2 or 3-point adjustment, taking into account the corresponding density and associated scale results. The setting for ethanol 75 %25 or glycerol 50 %25 is completed using an internally determined factor that takes into account the density.
"On the Xplorer, the pipette can be adjusted to different liquids or geographic heights using 1, 2 or 3-point adjustment, taking into account the corresponding density and associated scale results. The setting for ethanol 75 %25 or glycerol 50 %25 is completed using an internally determined factor that takes into account the density. "
Carbohydrates, aromatic groups, phenols and peptides have an absorption maximum of 230 nm.
Because the Biuret method can also be carried out at 562 nm (the absorption behavior of the Biuret reagent is almost identical in the range from 530 to 570 nm), you can measure your Biuret method on the BioPhotometer using the BCA method. Please note that you need only work with a standard for the Biuret method. The measurement of a standard curve is unnecessary.
Please check whether:
- the correct concentration unit was selected (e.g. mg/ml was chosen, but the sample has a concentration in the µg/ml range)
- an entry with decimal places was made for the standard concentrations. With an entry without decimal places, lesser sample concentrations (e.g. 0.024) cannot be recognized.
It is 3 m long.
Yes. For each colorimetric calibration saved on the BioPhotometer, all calculated parameters, such as, for example, the ascent of the calibration lines, are automatically filed in the affiliated calibration report. To call up the calibration report press the Function key, select "Calibration report" with the cursor, press the Enter key, select the desired calibration with the cursor, press the Enter key.
Please check whether the factor 1.000 is found under "Parameter" on the BioPhotometer. It is possible that the factor was accidentally reset to nil when working with the BioPhotometer. In case you continue to have difficulties with measurement, please contact our Application-Hotline: Tel. +49 180 3 66 67 89; email: email@example.com.
In general, the following applies: F = 1: (e x l) where F = factor [(µg/ml)-1 cm-1], e = molar extinction coefficient [M-1 cm-1], l = optical layer thickness of cuvette [cm]. For small molecules like oligonucleotides, for example, the correct extinction coefficient is determined from the base composition. As the concentration of oligonucleotides is commonly reported as mmol/liter, a millimolar extinction coefficient (E) is conventionally used in the Beer-Lambert equation by means of: E = A (15.3) + G (11.9) + C (7.9) + T (9.3) A, G, C and T here stand for the number of corresponding bases in this oligonucleotide, the numbers in brackets for the molar extinction coefficient of any deoxynucleotide at pH 7.0.
Source: Sambrook et. al. Molecular Cloning (2001).Third Edition, A8.21
Yes. Double-stranded ring-shaped DNA can be measured on the BioPhotometer using the "dsDNA" method, and single-stranded ring-shaped DNA with the "ssDNA" method.
You fill a cuvette with water and measure it as blank. Now fill another cuvette with water as well and measure this cuvette as a sample. The difference in absorption between the two cuvettes can now be identified as positive or negative absorption at the individual wavelengths. The most reliable method is to repeat the sample measurement up to three times and then to calculate the cuvette error as the mean value of the absorption differences measured.
Dust in the cuvette shaft can be carefully removed with a cotton tip. If the cuvette shaft is heavily soiled, for example with dried-on buffer residues, the cotton tip should be carefully soaked in 40% methanol beforehand.
Silicium photo diodes are used as detectors in the BioPhotometer.
The life span of the xenon lamp is influenced by neither repeated switching on and off nor by leaving it on in standby over a period of several hours. You can therefore decide for yourself. The xenon lamp is also only active during the measurement. It then switches over into standby mode.
Please note that you should measure at 260 nm for a minimum absorbance of 0.1 in order to obtain exact results. This value is independent of the measuring device and is based upon the disturbing influence of impurities and particles on the measurement result, which is especially high at A < 0.1. An A260/A230 < 2.0 can indicate impurity of the DNA sample. This is because peptides, aromatic groupes, phenols and carbohydrates absorb at 230 nm. With contaminated DNA samples you can try to clean the sample through centrifugation (at least. 5 min). Otherwise the sample must be purified again according to the protocol specifications.
Yes, if you are aware of a factor for double-strand RNA. You should enter this under the RNA method before the measurement under "Parameters". If you don't have a factor, you can carry out a calibration with a familiar dsRNA quantity and then use this to measure the unknown sample.
For purposes of data documentation, either a printer or a PC can be connected to each BioPhotometer. It is also possible to have the photometric systematic (trueness) and photometric random error (precision) of your device inspected by one of our service partners. For purposes of testing directly, we offer the user a Secondary UV-VIS-Filter.
Yes, the software is valid for the BioPhotometer plus as well as for the BioPhotometer 6131!
Yes, this is possible. You need an RS232/USB connection cable. This cable is worldwide available by the company VScom.
Please check, if all systems settings as stated in the manual in chapter 2.3 are correct.
Yes, this is possible. Click in the "work" area on "ID". Here you can connect you results to a specific series of measurement!
Yes, this is possible. Therefore you can use in the area "archive" the filter function.
No, this data will be exported automatically.
Yes, this is possible! Just click on top menu bar on "file" and "settings". In the right window you can apply a new user profile with a new password. Please note that only a new profile can apply if you are login as an administrator.
The BDTS will only be offered in English language.
No, the BDTS will only be available for Windows.
Negative A230 values are usually caused by interference components in inadequately concentrated DNA solutions. The negative value should be rectified when you use a lesser sample dilution for the next measurement. Please note that you should measure at 260 nm for a minimum absorbance of 0.1 in order to obtain exact results. This value is independent of the measuring device and is based upon the disturbing influence of impurities and particles on the measurement result, which is especially high at A < 0.1.
This measuring method is principally non-linear, when one observes the absorption curve over wide OD ranges and dilutions. The values are dependent on both the organism (size, shape) and the device (light path geometry, ratio of measured stray light radiation). A correct procedure includes the generation of a calibration graph for each organism and device by determining the actual number of cells under a microscope. When the curve shape is known it is then possible to measure in ranges that are almost linear.
Conversion is possible through calibration graphs or counting. With measurements of Escherichia coli, for example, it is possible to estimate roughly that an OD 600 between 0.5 and 1.0 corresponds approximately to a cell number between 1 x 108 and 1 x 109.
Please check whether
1.The absorbance of your DNA sample is greater than/equal to 0.1 (please note that regardless of the device used, absorption measurements of nucleic acids only deliver reliably reproducible results at absorbances of greater than 0.1. This is based upon the disturbing influence of impurities and particles on the measurement result, which is especially high at A < 0.1.)
2. Your sample was sufficiently mixed (only careful mixing of the sample can guarantee consistent measurement values)
3. There is an impurity in your sample. An indicator of a possible impurity is the relative ratio of A260/A280 or A260/A230, which with pure DNA should amount to app. 2.0 or 2.5 at pH 7-8.5. Carbohydrates, aromatic groups, phenols and peptides have an absorption maximum of 230 nm, proteins of 280 nm.
Additional literature: "Tipps and Tricks for photometric quantification of nucleic acids".
Please switch the BioPhotometer off and then on again following the installation of the program. The header line will then appear.
Wilfinger et. al* generally recommend using 1 - 3 mM Na2HPO4, pH 8.0 - 8.5 as the measuring medium for nucleic acids. For DNA measurements, our experience shows that 10 mM Tris-HCl, pH 8.0 or a TE buffer, pH 8.0 are also suitable. *Wilfinger W.W., Mackey K. and Chomczynski P. 1997. Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity. BioTechniques 22: 474-481.
The Bradford reagent is an aggressive substance that attacks the plastic of plastic cuvettes when the exposure time is too long. This reagent should therefore not remain in a plastic cuvette for more than 5 minutes. Fluctuating values could be caused by the fact that the Bradford reagent has already been too long in the cuvette.
Tip: Use glass cuvettes for the Bradford method.
Dimensions (WxHxD) = 160 mm x 70 mm x 170 mm; Weight app. 400 g.
The Thermal Printer is delivered equipped with an appropriate cable. Replacement cables are available from us upon request.
From software version 1.20 on you can also connect other serial and parallel printers (a converter cable is required) besides the Thermal Printer DPU 414 to the BioPhotometer. If you use the BioPhotometer with software version 1.0, you can only connect the Thermal Printer DPU 414.
Please send your Secondary UV-VIS-Filter set to your local Eppendorf partner.
From software version 1.20 on you can transfer measuring values from the BioPhotometer to a PC (respectively, in an Excel table) with the help of the BioPhotometer software package and then process them further or print them out.
We recommend checking the photometric systematic error (photometric trueness) and the wavelength systematic error (wavelength trueness) once a year using the Secondary UV-VIS-Filter set.
You can save one standard and one micro method for each protein determination method. This means that you can store two calibration curves for each protein determination method.
That depends upon the cuvette chosen. We recommend using the UVette with a minimum volume of 50 µl.
The BioPhotometer should not be used to measure an absorbance of less than 0.05 at 260 nm. This OD value corresponds to a dsDNA concentration of app. 2.5 µg/ml (= app. 125 ng dsDNA in a 50 µl preparation). Please note that you should measure at 260 nm for a minimum absorbance of 0.1 in order to obtain exact results. This value is independent of the measuring device and is based upon the disturbing influence of impurities and particles on the measurement result, which is especially high at A < 0.1.
The BioPhotometer should not be used to measure an absorbance of less than 0.05 at 260 nm. This OD value corresponds to a RNA concentration of app. 2 µg/ml (= app. 100 ng RNA in 50 µl preparation). Please note that you should measure at 260 nm for a minimum absorbance of 0.1 in order to obtain exact results. This value is independent of the measuring device and is based upon the disturbing influence of impurities and particles on the measurement result, which is especially high at A < 0.1.
Yes. This is possible with the Bradford/Bradford micro, Lowry/Lowry micro and BCA/BCA micro methods.
Factor F is calculated from the molar absorbance coefficient of a molecule in solution and the optical path length of the cuvette used (see Beer-Lambert law). The absorption behavior of nucleic acids is influenced by the aromatic rings of the bases. Here the molar absorbance coefficient of a double-stranded nucleic acid molecule with which the bases are in close contact with each other, is smaller than of a single-stranded nucleic acid molecule. It thus follows that single-stranded nucleic acid molecules have a higher rate of absorption than double-stranded molecules (Sambrook et al. 2001. Molecular Cloning 3rd edition, A8.20). With an optical path length of 10 mm and under neutral to slightly alkaline measuring conditions an optical density of 1 at 260 nm thus corresponds to approx. 50 µg/ml of double-stranded DNA, 37 µg/ml of single-stranded DNA, 40 µg/ml of RNA and appr. 30 µg/ml of oligonucleotides.
The 260 nm and 280 nm filter samples are for the testing of the wavelength accuracy, the A1, A2 and A3 filter samples for testing of the photometric accuracy (systematic and random error) of all wavelengths. The values measured with these filters must lie within the given limits. The limiting values are contained in a table found on the inside of the lid of the filter box.
As of software version 1.20 you can easily and comfortably transfer measurement data into a table calculation program such as Excel using our BioPhotometer software package. The PC online program also enables the use of the familiar printout design from the thermoprinter with a PC printer.
Precision measurement should be carried out using a semimicro-quartz glass cuvette (> 350 µl). Clear and clean solutions are suitable as the measuring medium, for example potassium dichromate (zero adjustment to water) or water (zero adjustment to air). The coefficient of variation (CV) should be smaller/equal to 1% at 1 A in line with the technical data for the BioPhotometer. Please bear in mind that the Secondary UV-VIS filter should be used for exact precision measurement and determination of both the photometric and wavelength accuracy.
It is 2 m long.
You can of course vortex and centrifuge your sample and then transfer it to a cuvette. Please make sure that the sample is transferred to the cuvette air bubble-free .
This is caused by the measurement outside of the upper measuring range (> 3.0 A).
1. Whether your cuvette is pressed firmly onto the base of the cuvette shaft. The error message "++++" indicates that the cuvette base is blocking the light beam.
2. Whether your cuvette has an optical window at 8.5 mm.
3. Whether you have sufficient blank medium in the cuvette.
Yes, in the method group “Routine” are all methods that also can be found in the BioPhotometer plus. These methods are the detection of nucleic acids, proteins, fluorescent dye labeled biomolecules plus OD600 measurements.
All cuvettes with standard dimensions can be used (12.5 * 12.5 mm). It is important that at height of 8.5 mm the optical window is transparent for the light beam)? Please check therefore also the specification in the manual (chapter 5.2).
"Yes, this is possible. To consider the light path for the calculation of the sample concentration, this value has to be entered in the area “check parameters”. Please note, that for the use of the TrayCell a sufficient sample concentration has to be applied: For the available sample concentration we recommend the absorbance ranges (e.g. dsDNA): 0.1 mm = 500 – 5000 ng/µL 0:2 mm = 250 – 2500 ng/µL 1 mm = 50 – 500 ng/µL 2 mm = 10 – 100 ng/µL These concentration ranges for dsDNA correspond to an absorbance of 0.1 to 1 A. "
Yes, this is easily possible. However, for absorbance measurement below 220 nm you have to consider the self absorbance of the UVette. For this reason we recommend to carry out measurements in the UVette only above 220 nm.
Basically every cuvette with standard dimensions can be used for the BioSpectrometer kinetic (s. chapter 5.2 in the manual). For optimal temperature control it is important that the cuvette has direct contact with the integrated peltier-element. Most adequate cuvettes are made of glass or quartz glass. In chapter 5.3.4 of the manual an overview is shown regarding pre-incubation times for different cuvette shapes.
Because of the shape of UVette the temperature transfer is here limited we recommend conventional cells made of glass or quartz glass!
In the method, "Advanced kinetics”, the measurement of standards can be programmed. This can be used for the measurement of unknown substrate concentrations via enzyme kinetics. Moreover it is possible if a drift of the reagent solution is expected, a “reagent blank” can be measurement. The result of this measurement is than subtracted from the sample measurement. These measurements are in the method "Simple Kinetics" not possible.
No, this is due to patent reasons not possible. But you can export the graph data as an Excel file and then reprocess here accordingly.
No, also this function is due to patent reasons not possible.
The files will be exported as an Excel file.
No, this is unfortunately not possible. But it is possible to export the data to an USB-Stick. The data can then be printed from the PC over a local or network printer.
A re-import of methods is unfortunately not possible.
For the BioSpectrometer an additional filter set will be available.
Maybe not. This is because the design of each piezo device is different e.g. distance of piezo element and the capillary, installation of capillary holder and size of piezo element. Usually, parameters with lower strength of PiezoXpert are used compared to other piezo devices due to its optimized design.
"Intensity stands for the level of pulse strength or impact. It can be set on a scale from 1-50 for program setting and 1-86 for the Clean function. The max. intensity of 86 equals 732 V."
The spacer plate functions as a “spacer” so that the actuator is not in direct contact with the micromanipulator. If it was, the piezo impulses could not be transferred directly and reproducibly onto the attached microcapillary.
"The use of liquid with high density is to give extra “weight” on the capillary that gives higher impact of piezo impulses for a successful perforation."
"Filling of mercury into the capillary can be done by backfilling using some special syringe e.g. Hamilton syringe. Usually 3-4 μL of mercury is sufficient. Note that mercury is toxic, please backfill the capillary under a fume hood."
Yes, it is normal. The design of the actuator element is optimized (directly adjacent to the capillary and rigid installation) in order to give efficient impulses directly to the target cell. This way, vibrations that could cause cell lysis are reduced. However, you can hear the impulses and also get a visual feedback via the illuminated rings around the buttons directly on the device.
"Make sure the capillary is inserted deep enough into the grip head. The capillary should be inserted until it touches the stopper inside the holder in order to allow direct transmission of the impulses onto the capillary."
"Improve the control of the micromanipulator. The use of an electronic micromanipulator where speed can be reduced helps. Reduce the strength of the piezo impulses by decreasing the settings for int. and speed."
The cylinder is made of polypropylene (PP) and the piston is made of polyethylene (PE).
Random samples are removed from each batch of Combitips and tested with a calibrated Repeater plus.
The Combitips advanced are available with the purity grades "guaranteed quality" "pcr clean"(not available in North America) and "biopur"
We are happy to send you a quality certificate for Combitip standard goods. Since Combitips are also available in Eppendorf PCR-clean and Eppendorf Biopur quality, a batch-specific Eppendorf certificate is also available
No. Although they are packed straight from production, they are not specially sterilized. If you want sterile Combitips, we recommend our Biopur Combitips.
No. The Combitip advanced is made of two materials which behave differently during autoclaving. This could lead to leakage which effects the performance of the Combitip
Yes they are. This information is also given on the corresponding certificate.
This Combitip advanced requires the red adapter, Ord. No. 0030 089.715, Biopur quality Ord. No. 0030 089.731.
For this Combitip advanced the gray Adapter is needed. Order-Nr. 0030 089.723, Biopur Quality, Order-Nr. 0030 089.740
The adapter is made of polypropylene (PP). PP is resistant to sodium hypochlorite for a very long time at room temperature.
No, you can also work with Combitips that are not completely filled. Please note that the 1st dispensing must also be discarded in this case.
The materials used to produce Combitips (50 ml, 25 ml, 10 ml, 5 ml, 2.5 ml, 1 ml and 0.5 ml) are compliant with the requirements on materials used for articles or components of articles intended to come into contact with food as described in:
France: Repression des Fraudes (1997), No 1227, et Supplément N° 1
EU: Commission Directive 90/128/EEC, 92/39/EEC, 93/9/EEC, 95/3/EC and 96/11/EC, Section A
USA: FDA, CFR, Title 21 (1998), 177.1520(a)(3)(i)(c)(1), (b) and (3)3.1a Olefin polymers.
Detection limits for monomers and additives used are not included in the directives mentioned. As Eppendorf does not use any additives and/or monomers in the production of Combitips, there should not be any problems.
These liquids have not been tested separately. The test result for petrol, which contains both these components, shows that use at room temperature is possible for 10 min to several hours. It should therefore be possible to pipette these substances.
Yes, however bleaching of the scale could occur in the long term.
Combitips advanced are resistant to most moderate inorganic acids. For example, 10% nitric acid, 60% sulphuric acid, 40% hydrofluoric acid and 35% hydrochloric acid can be taken up without damaging the Combitip and can be dispensed over a period of several weeks. We are not, however, able to give any information about dispensing accuracy or functionality of liquid dispensing. Also, we are not able to make any generally applicable statements for organic acids since the resistance varies considerably, depending on the acid. For example, the Combitip is resistant to 50% acetic acid at room temperature, but not to butyric acid.
This substance was tested separately. The test result for trifluoroacetic acid with a concentration below of 50% shows that Combitips can be used. Therefore higher concentrated trifluoroacetic acid (> 50%) the resistant is dependent on the exposure time.
This substance was not tested separately. The test result for ethylene glycol on PE and PP shows that Combitips can be used. Pipetting of PEG should therefore also be possible.
The primary components of petroleum spirit are pentane, hexane and octane. Because the secondary components are unknown, we cannot guarantee that the Combitips are suitable for your application. However, we would be pleased to send you a sample of the Combitips to try out.
Yes. For this we recommend the "Combilong". It consists of a long suction tube that can be cut to the corresponding bottle depth. A reservoir is filled through this tube from which liquid can be drawn easily with the Combitip.
Ordering-Nr. 0030 059.506
The Combitip rack is made of polypropylene (PP) and is autoclavable.
The Combitips adapter are wear parts. They should be changed regularly. Worn-out adapters might get caught when ejecting the combitip.
Yes, Combitips advanced are produced without using slip agents.There is a batch independant certificat available.
Yes, there is a lot unspecific certificat available.
Yes, there is a lot unspecific certificat available.
Due to the different rotors and adapters, a wide variety of tubes can be centrifuged with the
Centrifuge 5427 R:
• Micro test tubes (0.5 - 2.0 mL)
• Micro test tubes (5 mL)
• PCR strips and 0.2 mL PCR tubes
• Spin columns (1.5/2.0 mL)
• Microtainer (0.6 mL)
Spin columns or small filtration columns, for example for centrifugation of DNA or RNA, can be used in all rotors for 1.5/2.0 mL micro test tubes.
In particular, however, the kit rotor F-45-24-11-Kit is specially suitable for such spin columns, as open tube lids are supported by the raised edge of this rotor type and are no longer torn off (e.g. during elution of the sample from the column into a micro test tube).
Up to 48 0.2 mL PCR tubes (or four 8-tube PCR strips) can be centrifuged in the PCR rotor F-45-48-5-PCR. Furthermore, 0.2 mL PCR tubes can be used in each rotor for 1.5/2.0 mL micro test tubes using an adapter. (Order No. International 5425 715.005; North America 022636260).
0.5 mL micro test tubes can be centrifuged in all rotors for 1.5/2.0 mL micro test tubes using an adapter (Order No. International 5425 716.001; North America 022636227).
Up to 16 5 mL micro test tubes can be centrifuged in the aerosol-tight rotor for 5mL micro test tubes FA-45-11-17 (Order No. 5409 700.006).
One advantage of centrifugation with the swing bucket rotor is the possibility of sedimenting molecules that cannot be pelletized with a fixed-angle rotor (Microgradient, e.g. blood / Ficoll).
Firstly, the aerosol-tight rotors can be recognized by their designation abbreviation “FA” or “AT”; secondly, we have color-coded the aerosol-tight rotors.
All aerosol-tight rotors have a red rotor lid screw, and a red ring is printed on to the rotor shaft.
Aerosol-tight rotors are always recommended or even prescribed when working with hazardous or potentially hazardous substances. These can, for example, be viruses, pathogenic bacteria, potentially infectious blood samples or radioactive sample material.
At Eppendorf, the safety of the user always has the highest priority. That is why we now fit our centrifuges with aerosol-tight rotors as standard – at no extra charge.
The Centrifuge 5427 R has an automatic rotor recognition system. A newly installed rotor is recognized at the beginning of the run. The maximum speed of each rotor is automatically limited, if necessary, to prevent over-speeding of the rotors.
The SOFT function of the Centrifuge 5427 R allows slower and gentler acceleration and braking compared to the normal acceleration and braking times. As a result, delicate samples are not stressed unnecessarily. In addition, the risk of the pellets being shaken up again during braking is minimized.
Yes. It is possible to display the centrifugation speed in both rpm and rcf on all Eppendorf centrifuges. In line with the new operating concept of the centrifuges 5418, 5424, 5424 R, 5427R, 5430 and 5430 R, the units now have a separate rpm/rcf conversion button.
The Centrifuge 5427 R can be cleaned with neutral detergents. For disinfection, we recommend alcohol or alcoholic disinfectants. Make sure that the relevant legal requirements and directives are observed when choosing the disinfection method.
Yes, in the event of a power failure, the unit can be opened by means of the rotor key. Before the centrifuge is opened, always remove the mains plug from the plug socket and wait for the rotor to come to a standstill. For the emergency release of the centrifuge lid, the small plastic cap on the right front corner of the 5427 R has to be removed. Then insert the rotor key into the nut underneath the cap and open the lid latches by turning in anticlockwise direction.
The "Fast Temp" function is a temperature control run of the Centrifuge 5427 R without samples and with a rotor and temperature-specific rotational speed in order to quickly bring the rotor chamber to the set nominal temperature.
In order that the samples can be centrifuged from the beginning on at the desired temperature, one starts this temperature control run during sample preparation by pressing the "Fast Temp" key. The display shows the current temperature and g force (rcf) or speed (rpm) as well as “FT” as symbol for a “Fast Temp” temperature run. You can find a more detailed description in the operating instructions of the Centrifuge 5427 R.
The continuous cooling starts with each rotor standstill of the Centrifuge 5427 R in order to maintain the preselected nominal temperature of the rotor chamber not only during centrifugation. To this purpose the following prerequisites must be met:
- The centrifuge is switched on.
- The centrifuge lid is closed.
- The nominal temperature is lower than the ambient temperature.
- The centrifuge is not in standby mode.
Continuous cooling is ended by opening the centrifuge lid or by pressing any key.
The lowest temperature of the Centrifuge 5427 R is -11 °C. The device is thereby optimized with all rotors in such a way that at least 4 °C is maintained with all rotors at the appropriate maximum speeds.