Yes, the batch-specific purity grades can be downloaded at www.eppendorf.com/certificates.

The previous purity grades were developed due to customer requirements and new applications, in the course of the years. To meet the customer requirements for single applications even better, Eppendorf has extended the certification of the purity grades "Sterile" (now, also certified as free of pyrogenes) and "Biopur" (now, also certified as free of DNase and PCR inhibitors). In addition, the previous purity grades have been transferred to a new modular system that provides the same variety and flexibility and yet is readily understandable.

For all purity grades, there will be new packing seals. In addition, there will be a further certification for the purity grades "Sterile" and "Biopur". "Sterile" will certify sterility and also the freedom of pyrogenes. The purity grade "Biopur" also includes the freedom of DNase as well as of PCR inhibitors.

Yes, they can be autoclaved once at 121 °C, 1 bar.

Yes.

The outer diameter is 0.3 mm and the inner diameter is approx. 0.12 mm.

The adapter is made of polypropylene (PP). PP is resistant to sodium hypochlorite at room temperature over very lengthy periods.

The epTIPS Biopur are suitable for use in the food industry according to the Food and Consumer Goods Legislation § 5, paragraph 1 no. 1 (food and consumer goods). Please note: The suitability of a consumer item for the intended purpose, the influence of the filler on taste and smell, and the compliance with limitations (e.g. global migration, specific limitations and other analytical specifications) should be tested by the manufacturer or user for individual cases.

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

All Biopur products are manufactured fully automatically under cleanroom conditions. This eliminates contamination of the disposables through contact with people. Additionally the products are sterilized by radiation or treatment with ethylene oxide. An independent laboratory checks that each individual batch is free of pyrogens.

Contamination and cross-over can only be avoided by the use of single-use plastic items. In addition there is no annoying and time-consuming cleaning of glassware.

Yes, a general quality certificate is available for epTIPS, which is included in the delivery package.

Like all our single-use plastic items, our pipette tips are also made of polypropylene and can therefore be stored at -10°C even for many years. Please note that you should keep the pipette tips for several minutes at room temperature before pipetting.

Organic, heavy-metal-free dyes (PP granulate) are used to tint the pipette tips.

Yes. Use on a sterile bench, for example, is therefore completely safe.

The tips are electrically neutral. There are no charged particles on the surface. It is therefore unlikely that charged particles like ions or ionic compounds will get caught on the walls.

No.

Yes, this is clear from the details about substances contained in the safety/technical data sheets.

The epTIPS of PP are suitable for liquids with a temperature of max. 90°C.

No, we advise against this application.

The box can be autoclaved up to 100 times, the reloads up to 5 times.

The geometry of the epBox housing changes when the box(es) is (are) forced into the autoclave and the door of the autoclave can only be closed with a degree of force. Once the autoclave has cooled down, the deformed shape is retained. Stacking of many boxes should also be avoided. What is more, autoclaving temperature should be checked regularly. Experience suggests that autoclaves are frequently operated at excessive temperatures. The permissible temperature quoted by Eppendorf should never be exceeded.

No, the epTIPS Box cannot be autoclaved at 133 °C. We recommend 121 °C.

The UV stability of the existing epTIPS Box of polycarbonate and of the existing epTIPS Racks of polypropylene were tested during development. These were thereby irradiated with a lamp of 254 nm and energy of approx. 1 J/cm2 for 16.7 hours. After 10 cycles, thus a total of 167 hours, there were still no signs of adverse effects on the box material. Because the energy of the lamp used is considerably higher than that of a lamp used in a sterile bench, it can be assumed that both boxes can be exposed to UV radiation for at least 300-600 hours in a sterile bench without problems. Because it is well known that polypropylene is at least 3 times more stable against UV radiation than polycarbonate, correspondingly longer UV stability under sterile bench conditions can be presumed for the epTIPS Racks.

"It indicates sterilization by radiation. This indicator changes from orange to red when the product has been sterilized with radiation as planned. "

The non-amber colored Eppendorf Safe-Lock Tubes are UV resistant in the sense that DNA samples can be processed in them without danger of DNA chain damage. Samples are generally stored in the refrigerator or in an ice bath, and are therfore usually only very briefly exposed to daylight. We recommend the use of amber colored tubes for particularly light-sensitive samples.

All of our tubes are manufactured from polypropylene. The polypropylene we use fulfils the specifications of the FDA for the use of plastics for foodstuffs. No metal-based dyes are used.

"Yes, this is possible. Please note that the micro test tubes have to be taken out of the packaging for sterilization. In addition, please note that some ethylene oxide will remain in the material after sterilization. "

"Yes, the tubes can withstand UV polymerization. However, we cannot give you any instructions for the procedure."

"All micro test tubes designated with "PCR clean" are free of human DNA, DNase, RNase and PCR inhibitors. Our Safe-Lock micro test tubes are also available in the "Eppendorf Biopur" purity standard. Due to the special manufacturing processes and the individual blister-packaging, Biopur products are certified "DNA free"."

"Definition of RT (room temperature): +5 to +35 °C."

Lead, cadmium, mercury, etc. are well below the prescribed limits of the DIN norm. In the course of the production process it is impossible to avoid the smallest quantities of metal resulting from machine friction.

No. All our test tubes are made from pure polypropylene. The material does not contain PEG.

The amber colored Eppendorf Safe-Lock Tubes protect from light in the spectrum from 700 nm to the UV spectrum of 190 nm. In the spectrum of visible light (350 - 600 nm) the protection is optimal with lessening intensity. At app. 550 nm (excitation wavelength for Cy3) the measured deletion is app. 1, meaning that 90 % of the incident light is blocked by the walls of the tube. At 650 nm (excitation wavelength for Cy5) the measured deletion is app. 0.35, meaning that app. 65 % of the incident light is blocked by the walls of the tube. If that is not sufficient, we recommend wrapping the tube in aluminum foil.

"Yes. The tube material could shrink somewhat, but this should have no negative effect on the tube seal. "

"The Micropestle is manufactured from polypropylene."

The Rack 3821 is manufactured from noryl, a polyphenyl ether modified with polystyrene.

No. The propylenes which we use are materials of foodstuff quality. It is tested according to the directives for materials which come into contact with foodstuffs of the German Federal Office of Health and has no nitrogen-containing components.

Yes. They can be autoclaved (1 bar, 20 minutes, 121 °C).

All plates listed in the Eppendorf catalog can be sealed with the heat sealer.

The heat sealer from Eppendorf in combination with heat sealing foils or films offers the best sealing. Another effective method is to use Eppendorf cap strips. For sealing with cap strips, we recommend using the capping aid, an inexpensive tool for producing a very effective seal as cannot be obtained by setting on the caps with the fingers alone.

Both tips have the same measurements. The length is 242 mm, the upper base has a diameter of app. 10.5 mm, the diameter of the Maxitip is 10 mm. The diameter of the somewhat elongated tips (25 mm long) is 4.5 mm.

In comparison to a Eppendorf Safe-Lock Tube, the DNA LoBind Tube shows significantly less DNA bonding affinity. This effect is most apparent with high salt concentrations and when working with short DNA fragments. The DNA LoBind Tube is therefore especially suitable for applications that should be carried out with the minimum loss of nucleic acid material.

Not the Eppendorf LoBind Tubes are neither siliconized nor coated. The low binding affinity results from of a special type of polypropylene and the optimized manucturing process.

Yes, but the dimensional precision of the plates can be affected. If the use of sterile plates is needed we recommend Eppendorf Deepwell Plate Sterile.

Eppendorf Deepwell Plate Standard and LoBind Quality can be centrifuged up to 6000 x g. Eppendorf Deepwell Plate Sterile withstand up to 5000 x g. For details refer to the instruction manual.

No – Eppendorf Deepwell Plate are made of polypropylene which does not contain plasticizers.

Eppendorf Deepwell Plate are of polypropylene. All parts of the plate (colorless/colored parts and coding) have the same high resistance against chemicals and are resistant against many organic solvents. For details please see our Application Note no. 56: The best material for original Eppis. In case of doubt please contact Eppendorf Application Support: support@eppendorf.com.

No - we recommend storage at –80°C.

These tips will grant an advantage especially during pipetting of solutions containing detergents, such as PCR master mixes, enzyme solutions and many buffers. The LoRetention tips are characterized by significantly improved flow performance, thus achieving more economic use of expensive reagents, while valuable sample material is used effectively. Furthermore, reproducibility is improved, since wetting of the tip is dependent on both user and handling (for example: pipetting speed) and can therefore vary considerably.

The LoRetention tips are made from polypropylene, as are standard tips, and they are resistant to a number of organic solvents. Further information regarding chemical resistance properties of pipette tips can be found in the User Guide no. 23: Chemical Stability of Consumables.

Yes. Foam formation is rarely observed with the use of LoRetention tips. This facilitates the handling of these solutions and increases reproducibility.

The Eppendorf PP Microplates are made from polypropylene and are thus resistant to DMSO and other organic solvents, unlike plates made from polystyrene. An overview of chemical resistance can be found in Application Note no. 56. In case of doubt please contact Eppendorf Application Support: support@eppendorf.com.

Eppendorf offers a variety of sealing options for Microplates. Self-adhesive sealers, films and foils for heat sealing, as well as re-usable sealing mats, are available. Information regarding the compatibility of the different sealing methods is listed in the Application Note no. 208.

Plates made from white material may be used for luminescence assays (chemiluminescence, scintillation proximity assays (SPA) etc.), as well as for fluorescence assays (fluorescence intensity, polarization, etc.). The white wells prevent “cross talk” between the wells while amplifying the individual signals. The white Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in white plates. Due to carefully selected materials, the plates offer maximum signal amplification. Due to their very low autofluorescence, the white Eppendorf Microplates are equally well suited for fluorescence assays. Taken together, these features allow for an improvement in the sensitivity of analyses.

Yes; however, the exact specifications of the Microplates could be compromised. The plates are available in the qualities sterile or PCR clean. Hence, autoclaving is necessary only under exceptional circumstances.

The Eppendorf PP Microplates may be centrifuged up to 6000 x g. Please follow the instructions provided in the manual.

Typically, absorption measurements are not performed in plates made from polypropylene, but in plates made from polystyrene, as this material generally has a very high transparency. Since the Eppendorf PP Microplates show considerably higher transparency compared to other plates made from polypropylene (see Application Note no. 202), the Eppendorf Microplates may be used for absorption measurements in certain cases.

Yes, the option of Eppendorf Microplates with barcode is available. Further information can be found on the product page www.eppendorf.com/microplates.

Random samples are removed from each batch of Combitips and tested with a calibrated Repeater plus.

We are happy to send you a quality certificate for Combitip standard goods. Since Combitips are also available in Eppendorf PCR-clean and Eppendorf Biopur quality, a batch-specific Eppendorf certificate is also available

No. The Combitip advanced is made of two materials which behave differently during autoclaving. This could lead to leakage which effects the performance of the Combitip

This Combitip advanced requires the red adapter, Ord. No. 0030 089.715, Biopur quality Ord. No. 0030 089.731.

The adapter is made of polypropylene (PP). PP is resistant to sodium hypochlorite for a very long time at room temperature.

The materials used to produce Combitips (50 ml, 25 ml, 10 ml, 5 ml, 2.5 ml, 1 ml and 0.5 ml) are compliant with the requirements on materials used for articles or components of articles intended to come into contact with food as described in:
France: Repression des Fraudes (1997), No 1227, et Supplément N° 1
EU: Commission Directive 90/128/EEC, 92/39/EEC, 93/9/EEC, 95/3/EC and 96/11/EC, Section A
USA: FDA, CFR, Title 21 (1998), 177.1520(a)(3)(i)(c)(1), (b) and (3)3.1a Olefin polymers.
Detection limits for monomers and additives used are not included in the directives mentioned. As Eppendorf does not use any additives and/or monomers in the production of Combitips, there should not be any problems.

Yes, however bleaching of the scale could occur in the long term.

This substance was tested separately. The test result for trifluoroacetic acid with a concentration below of 50% shows that Combitips can be used. Therefore higher concentrated trifluoroacetic acid (> 50%) the resistant is dependent on the exposure time.

The primary components of petroleum spirit are pentane, hexane and octane. Because the secondary components are unknown, we cannot guarantee that the Combitips are suitable for your application. However, we would be pleased to send you a sample of the Combitips to try out.

Yes. At 121 degrees Celsius.

Yes, up to 50 times.

Yes, Combitips advanced are produced without using slip agents.There is a batch independant certificat available.

Yes, there is a lot unspecific certificat available.