No, the tips are uncoated. The ultra-hydrophobic surface is achievd through an innovative treatment at the molecular level.

The lids of the LoRetention racks and reloads have a transparent lid as compared to the blue lid of the standard tips. A reusable LoRetention adhesive sticker is placed on the lid. This sticker also features “LoRetention” writing on the reverse side. This way previously purchased epT.I.P.S. boxes can be labeled. When the box is open, the writing “LoRetention” will thus be easily readable.

Yes, the batch-specific purity grades can be downloaded at www.eppendorf.com/certificates.

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

No, they are the same products as before, i.e. all specifications as dimensions remain the same. Only the packing has changed, due to the new seals. In addition, the certification of "Sterile" and "Biopur" has been extended but the entire production process was maintained.

Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.

The Eppendorf PP Microplates are made from polypropylene and are thus resistant to DMSO and other organic solvents, unlike plates made from polystyrene. An overview of chemical resistance can be found in Application Note no. 56. In case of doubt please contact Eppendorf Application Support: support@eppendorf.com.

Eppendorf offers a variety of sealing options for Microplates. Self-adhesive sealers, films and foils for heat sealing, as well as re-usable sealing mats, are available. Information regarding the compatibility of the different sealing methods is listed in the Application Note no. 208.

Plates made from white material may be used for luminescence assays (chemiluminescence, scintillation proximity assays (SPA) etc.), as well as for fluorescence assays (fluorescence intensity, polarization, etc.). The white wells prevent “cross talk” between the wells while amplifying the individual signals. The white Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in white plates. Due to carefully selected materials, the plates offer maximum signal amplification. Due to their very low autofluorescence, the white Eppendorf Microplates are equally well suited for fluorescence assays. Taken together, these features allow for an improvement in the sensitivity of analyses.

Yes; however, the exact specifications of the Microplates could be compromised. The plates are available in the qualities sterile or PCR clean. Hence, autoclaving is necessary only under exceptional circumstances.

The Eppendorf PP Microplates may be centrifuged up to 6000 x g. Please follow the instructions provided in the manual.

Typically, absorption measurements are not performed in plates made from polypropylene, but in plates made from polystyrene, as this material generally has a very high transparency. Since the Eppendorf PP Microplates show considerably higher transparency compared to other plates made from polypropylene (see Application Note no. 202), the Eppendorf Microplates may be used for absorption measurements in certain cases.

Yes, the option of Eppendorf Microplates with barcode is available. Further information can be found on the product page www.eppendorf.com/microplates.

Lead, cadmium, mercury, etc. are well below the prescribed limits of the DIN norm. In the course of the production process it is impossible to avoid the smallest quantities of metal resulting from machine friction.

"The lower part of the Xplorer can be autoclaved. 175 autoclaving cycles (121 °C, 1 bar, 20 min.) were tested. The number of the corresponding tests corresponds to an average 5-year use of the pipette."

"Yes, acids can be dispensed with the Xplorer. But their higher density must be taken into consideration and the Xplorer should be adjusted to the changed physical data in the ""Options"" operating mode."

In the "Pipetting and mixing" operating mode, the mixing volume can be defined independent from the filling volume.

"A complete run with volume, speed, etc. can be saved at the program level. After exiting the program level, the run can no longer be changed just by pressing the keys. "

Based on a speed level between min. 2.6 S (Step 1) and max. 12 S (Step 8).

"The Xplorer technical data is only valid for pipetting. Conformity must be achieved for this operating mode. For piston-stroke pipettes, there are no requirements for dispensing accuracy according to EN ISO 8655."

"The Xplorer can be charged using the power supply included in the accessories or the optionally available charging stand or charging carousel. One Xplorer can be charged with the charging stand and four with the charging carousel. A single-channel or multi-channel Xplorer can be attached to a charging shell."

The li-ion battery features high energy density. Its usable service life is several years; but this is strongly dependent on usage and storage conditions.

Due to the reduction in dispensing speed and additional vacuums of the Blow out, the sample can be accurately dispensed in the gel-slots. The enormous synchronization of the Xplorer motor also achieves very good results. In the operating mode "Pipetting and mixing P/M," the sample is automatically mixed with the preparation after dispensing. The mixing cycles and mixing volume can be individually defined for this.

In the operating mode "Pipetting and mixing P/M," the sample is automatically mixed with the preparation after dispensing. The mixing cycles and mixing volume can be individually defined for this.

"On the Xplorer, the pipette can be adjusted to different liquids or geographic heights using 1, 2 or 3-point adjustment, taking into account the corresponding density and associated scale results. The setting for ethanol 75 %25 or glycerol 50 %25 is completed using an internally determined factor that takes into account the density. "

Because the Biuret method can also be carried out at 562 nm (the absorption behavior of the Biuret reagent is almost identical in the range from 530 to 570 nm), you can measure your Biuret method on the BioPhotometer using the BCA method. Please note that you need only work with a standard for the Biuret method. The measurement of a standard curve is unnecessary.

It is 3 m long.

Please check whether the factor 1.000 is found under "Parameter" on the BioPhotometer. It is possible that the factor was accidentally reset to nil when working with the BioPhotometer. In case you continue to have difficulties with measurement, please contact our Application-Hotline: Tel. +49 180 3 66 67 89; email: support@eppendorf.com.

Yes. Double-stranded ring-shaped DNA can be measured on the BioPhotometer using the "dsDNA" method, and single-stranded ring-shaped DNA with the "ssDNA" method.

Dust in the cuvette shaft can be carefully removed with a cotton tip. If the cuvette shaft is heavily soiled, for example with dried-on buffer residues, the cotton tip should be carefully soaked in 40% methanol beforehand.

The life span of the xenon lamp is influenced by neither repeated switching on and off nor by leaving it on in standby over a period of several hours. You can therefore decide for yourself. The xenon lamp is also only active during the measurement. It then switches over into standby mode.

Yes, if you are aware of a factor for double-strand RNA. You should enter this under the RNA method before the measurement under "Parameters". If you don't have a factor, you can carry out a calibration with a familiar dsRNA quantity and then use this to measure the unknown sample.

Yes, the software is valid for the BioPhotometer plus as well as for the BioPhotometer 6131!

Please check, if all systems settings as stated in the manual in chapter 2.3 are correct.

Yes, this is possible. Therefore you can use in the area "archive" the filter function.

Click top menu bar on "new archive file"!

The BDTS will only be offered in English language.

Windows XP or Windows Vista.

Negative A₂₃₀ values are usually caused by interference components in inadequately concentrated DNA solutions. The negative value should be rectified when you use a lesser sample dilution for the next measurement. Please note that you should measure at 260 nm for a minimum absorbance of 0.1 in order to obtain exact results. This value is independent of the measuring device and is based upon the disturbing influence of impurities and particles on the measurement result, which is especially high at A < 0.1.

Conversion is possible through calibration graphs or counting. With measurements of Escherichia coli, for example, it is possible to estimate roughly that an OD 600 between 0.5 and 1.0 corresponds approximately to a cell number between 1 x 108 and 1 x 109.

Please switch the BioPhotometer off and then on again following the installation of the program. The header line will then appear.

The Bradford reagent is an aggressive substance that attacks the plastic of plastic cuvettes when the exposure time is too long. This reagent should therefore not remain in a plastic cuvette for more than 5 minutes. Fluctuating values could be caused by the fact that the Bradford reagent has already been too long in the cuvette.
Tip: Use glass cuvettes for the Bradford method.

The Thermal Printer is delivered equipped with an appropriate cable. Replacement cables are available from us upon request.

Please send your Secondary UV-VIS-Filter set to your local Eppendorf partner.

We recommend checking the photometric systematic error (photometric trueness) and the wavelength systematic error (wavelength trueness) once a year using the Secondary UV-VIS-Filter set.

That depends upon the cuvette chosen. We recommend using the UVette with a minimum volume of 50 µl.

The BioPhotometer should not be used to measure an absorbance of less than 0.05 at 260 nm. This OD value corresponds to a RNA concentration of app. 2 µg/ml (= app. 100 ng RNA in 50 µl preparation). Please note that you should measure at 260 nm for a minimum absorbance of 0.1 in order to obtain exact results. This value is independent of the measuring device and is based upon the disturbing influence of impurities and particles on the measurement result, which is especially high at A < 0.1.

Factor F is calculated from the molar absorbance coefficient of a molecule in solution and the optical path length of the cuvette used (see Beer-Lambert law). The absorption behavior of nucleic acids is influenced by the aromatic rings of the bases. Here the molar absorbance coefficient of a double-stranded nucleic acid molecule with which the bases are in close contact with each other, is smaller than of a single-stranded nucleic acid molecule. It thus follows that single-stranded nucleic acid molecules have a higher rate of absorption than double-stranded molecules (Sambrook et al. 2001. Molecular Cloning 3rd edition, A8.20). With an optical path length of 10 mm and under neutral to slightly alkaline measuring conditions an optical density of 1 at 260 nm thus corresponds to approx. 50 µg/ml of double-stranded DNA, 37 µg/ml of single-stranded DNA, 40 µg/ml of RNA and appr. 30 µg/ml of oligonucleotides.

As of software version 1.20 you can easily and comfortably transfer measurement data into a table calculation program such as Excel using our BioPhotometer software package. The PC online program also enables the use of the familiar printout design from the thermoprinter with a PC printer.

It is 2 m long.

Yes.

This is caused by the measurement outside of the upper measuring range (> 3.0 A).
Please check
1. Whether your cuvette is pressed firmly onto the base of the cuvette shaft. The error message "++++" indicates that the cuvette base is blocking the light beam.
2. Whether your cuvette has an optical window at 8.5 mm.
3. Whether you have sufficient blank medium in the cuvette.

All cuvettes with standard dimensions can be used (12.5 * 12.5 mm). It is important that at height of 8.5 mm the optical window is transparent for the light beam)? Please check therefore also the specification in the manual (chapter 5.2).

Yes, this is easily possible. However, for absorbance measurement below 220 nm you have to consider the self absorbance of the UVette. For this reason we recommend to carry out measurements in the UVette only above 220 nm.

Because of the shape of UVette the temperature transfer is here limited we recommend conventional cells made of glass or quartz glass!

No, this is due to patent reasons not possible. But you can export the graph data as an Excel file and then reprocess here accordingly.

The files will be exported as an Excel file.

A re-import of methods is unfortunately not possible.

Maybe not. This is because the design of each piezo device is different e.g. distance of piezo element and the capillary, installation of capillary holder and size of piezo element. Usually, parameters with lower strength of PiezoXpert are used compared to other piezo devices due to its optimized design.

As speed we define the number of pulses per second.

"The use of liquid with high density is to give extra “weight” on the capillary that gives higher impact of piezo impulses for a successful perforation."

Yes, it is normal. The design of the actuator element is optimized (directly adjacent to the capillary and rigid installation) in order to give efficient impulses directly to the target cell. This way, vibrations that could cause cell lysis are reduced. However, you can hear the impulses and also get a visual feedback via the illuminated rings around the buttons directly on the device.

"Improve the control of the micromanipulator. The use of an electronic micromanipulator where speed can be reduced helps. Reduce the strength of the piezo impulses by decreasing the settings for int. and speed."

Random samples are removed from each batch of Combitips and tested with a calibrated Repeater plus.

We are happy to send you a quality certificate for Combitip standard goods. Since Combitips are also available in Eppendorf PCR-clean and Eppendorf Biopur quality, a batch-specific Eppendorf certificate is also available

No. The Combitip advanced is made of two materials which behave differently during autoclaving. This could lead to leakage which effects the performance of the Combitip

This Combitip advanced requires the red adapter, Ord. No. 0030 089.715, Biopur quality Ord. No. 0030 089.731.

The adapter is made of polypropylene (PP). PP is resistant to sodium hypochlorite for a very long time at room temperature.

The materials used to produce Combitips (50 ml, 25 ml, 10 ml, 5 ml, 2.5 ml, 1 ml and 0.5 ml) are compliant with the requirements on materials used for articles or components of articles intended to come into contact with food as described in:
France: Repression des Fraudes (1997), No 1227, et Supplément N° 1
EU: Commission Directive 90/128/EEC, 92/39/EEC, 93/9/EEC, 95/3/EC and 96/11/EC, Section A
USA: FDA, CFR, Title 21 (1998), 177.1520(a)(3)(i)(c)(1), (b) and (3)3.1a Olefin polymers.
Detection limits for monomers and additives used are not included in the directives mentioned. As Eppendorf does not use any additives and/or monomers in the production of Combitips, there should not be any problems.

Yes, however bleaching of the scale could occur in the long term.

This substance was tested separately. The test result for trifluoroacetic acid with a concentration below of 50% shows that Combitips can be used. Therefore higher concentrated trifluoroacetic acid (> 50%) the resistant is dependent on the exposure time.

The primary components of petroleum spirit are pentane, hexane and octane. Because the secondary components are unknown, we cannot guarantee that the Combitips are suitable for your application. However, we would be pleased to send you a sample of the Combitips to try out.

Yes. At 121 degrees Celsius.

Yes, up to 50 times.

Yes, Combitips advanced are produced without using slip agents.There is a batch independant certificat available.

Yes, there is a lot unspecific certificat available.

Spin columns or small filtration columns, for example for centrifugation of DNA or RNA, can be used in all rotors for 1.5/2.0 mL micro test tubes.
In particular, however, the kit rotor F-45-24-11-Kit is specially suitable for such spin columns, as open tube lids are supported by the raised edge of this rotor type and are no longer torn off (e.g. during elution of the sample from the column into a micro test tube).

0.5 mL micro test tubes can be centrifuged in all rotors for 1.5/2.0 mL micro test tubes using an adapter (Order No. International 5425 716.001; North America 022636227).

One advantage of centrifugation with the swing bucket rotor is the possibility of sedimenting molecules that cannot be pelletized with a fixed-angle rotor (Microgradient, e.g. blood / Ficoll).

Aerosol-tight rotors are always recommended or even prescribed when working with hazardous or potentially hazardous substances. These can, for example, be viruses, pathogenic bacteria, potentially infectious blood samples or radioactive sample material.

The Centrifuge 5427 R has an automatic rotor recognition system. A newly installed rotor is recognized at the beginning of the run. The maximum speed of each rotor is automatically limited, if necessary, to prevent over-speeding of the rotors.

Yes. It is possible to display the centrifugation speed in both rpm and rcf on all Eppendorf centrifuges. In line with the new operating concept of the centrifuges 5418, 5424, 5424 R, 5427R, 5430 and 5430 R, the units now have a separate rpm/rcf conversion button.

Yes, in the event of a power failure, the unit can be opened by means of the rotor key. Before the centrifuge is opened, always remove the mains plug from the plug socket and wait for the rotor to come to a standstill. For the emergency release of the centrifuge lid, the small plastic cap on the right front corner of the 5427 R has to be removed. Then insert the rotor key into the nut underneath the cap and open the lid latches by turning in anticlockwise direction.

The continuous cooling starts with each rotor standstill of the Centrifuge 5427 R in order to maintain the preselected nominal temperature of the rotor chamber not only during centrifugation. To this purpose the following prerequisites must be met:
- The centrifuge is switched on.
- The centrifuge lid is closed.
- The nominal temperature is lower than the ambient temperature.
- The centrifuge is not in standby mode.

Continuous cooling is ended by opening the centrifuge lid or by pressing any key.

With the Centrifuge 5427 R it is possible to set the temperature within a range from -11 °C to +40 °.