The basic unit of the Multiporator is made up of a module designed to electroporate eukaryotic cells.
Two optional function modules extend the application range of the Multiporator to either cell fusion or the transformation of bacteria and yeasts. No additional devices or external parts are necessary.
Together with the buffers, cuvettes and chambers that are precisely suited for the corresponding applications, the Multiporator forms the perfect system.
Electroporation of eukaryotic cells
Soft Pulse technology applies extremely short electric pulses for the highest survival rates. Cell-damaging influences, such as changes in pH values, aluminum release and electrophoresis of the cell content, are minimized. The relevant parameters of voltage and pulse duration are directly set, and the patented electronic pulse discharge ensures that they will be maintained exactly—independent of the sample resistance—for reliable and reproducible results.
The hypoosmolar buffer system enables the cell to swell up, thus enabling easier membrane penetration which leads to high transfection rates. The ion composition is adapted to the inner cell environment and stabilizes the Na+/K+ gradient across the cell membrane.
Applications include the transfection of animal and human cell lines, embryonic stem cells, primary cells, oocytes and plant cells.
Recent experiments have proven that the Multiporator is specially suited for the transfer of siRNA into cell lines and primary cells.*1, 2, 3
*1 Sergio Gonzalez, Daniela Castanotto, Haitang Li, Simon Olivares, Michael C. Jensen, Stephen J.
Forman, John J. Rossi, Laurence J.N. Cooper: Amplification of RNAi-Targeting HLA mRNAs
(Molecular Therapy, Vol. 11, 2005)
*1US Patent 6008038