Purification of genomic DNA from tissue culture cells in a high-throughput method is essential for many downstream applications in molecular biology. In this report, we have developed an automated method to meet the need for high-throughput genomic DNA purification on the Eppendorf® epMotion® 5075 VAC. Genomic DNA is purified from tissue culture cells with no detectable cross-contamination during the automated isolation procedure. Genomic DNA is then analyzed for yield and purity by measuring sample absorbance at 260 nm and 280 nm, amplified to confirm compatibility for downstream PCR amplification (1.2 kb IL-1ß), and compared to genomic DNA extracted from the same tissue culture cells using a manual method.

163