A new, automated method for high-throughput RNA isolation from tissue culture cells has been developed on the Eppendorf® epMotion™ 5075 VAC using the Promega Wizard® SV 96 Total RNA Isolation System. Total RNA is purified from cultured Jurkat cells with no detectable cross-contamination during the automated procedure. Yield and purity of RNA obtained using this method are determined to be of excellent quality as accessed quantitatively using the Quant-iT™ RiboGreen® RNA Assay and spectrophotometrically by relative absorbance measurements (i.e., A260/A280). Furthermore, successful RT-PCR amplification confirms compatibility of RNA isolated using this methodology for downstream applications.

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