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CelliGen® BLU — Single-Use, Stirred-Tank Bioreactor

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Application note 250: Introduction to CHO Culture in a Stirred-Tank Bioreactor	250	466 KB	Download
Application note 252: Introduction to Sf-9 Insect Culture in a Stirred-Tank Bioreactor	252	537 KB	Download
Application note 257: Single-Use Scalability: CHO Cell Culture using 5 to 50 L New Brunswick™ CelliGen® BLU Benchtop, Stirred-Tank Bioreactors	257	769 KB	Download
Application Note 254: CHO Cell Culture with Single-Use New Brunswick™ CelliGen® BLU Packed-Bed Fibra-Cel® Basket	254	793 KB	Download

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CelliGen® BLU — Single-Use, Stirred-Tank Bioreactor

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The objective of this study was to compare New Brunswick CelliGen BLU single-use packed-bed bioreactor and the traditional glass vessel counterpart used in New Brunswick CelliGen 310. Alkaline phosphatase (ALKP)- secreting Chinese Hamster ovary (CHO) cells were used to measure ALKP production in each bioreactor. Overall, the results from these comparisons suggest that there is no significant difference between the reusable and singleuse FibraCel basket systems for bench-scale production of recombinant proteins. Productivity of cells and collection of secreted proteins will not be hindered by the implementation of single-use bioreactor systems.