Microinjection into adherent cells - theory and practical exercises
Technicians and scientists interested in microinjection into adherent cells. Basic experience with cell culture and microscopy are required.
Objective of seminar:
By the end of the course, participants should be able to prepare and perform and analyse microinjection experiments into cultured cells using specific manipulators.
Content of seminar:
A – Microinjection techniques into adherent cells:
- Sample preparation
- Microinjection of marker dyes
- Microinjection of GFP-constructs
B – Theoretical and practical microscopy training
This part of the course will be supported by Carl Zeiss Microscopy
C – Analysis of the experiments with fluorescence microscopy techniques