Transgenic Animals - Micromanipulation Techniques
Technicians and scientists interested in the generation of transgenic animals. Basic experience with eukaryotic cells and microscopy required.
Objective of seminar:
By the end of the course, participants should be able to perform microinjection into mouse zygotes as well as transfer embryonic stem cells into mouse 8-cell embryos and blastocysts using a specific micromanipulation setup.
Content of seminar:
A – Microinjection techniques for transgenic mice:
- DNA/RNA Microinjection into zygotes
- Embryonic stem cell transfer into blastocysts
- Piezo-assisted stem cell transfer into 8-cell stage embryos
B – Theoretical and practical microscopy training:
- This part of the training will be supported by Leica Microsystems