ThermoMixer C; ThermoStat C: Is it possible to run more than one program in automatic succession?
No. Each program has to be chosen and run separately. But the 4 possible steps within a program run in automatic succession.
Which SmartBlocks and vessels can be used with ThermoMixers F?
The ThermoMixers F possess a fix installed SmartBlock and can only be used with the appropriate microtest tubes or plates, such as MTP’s, DWP’s or skirted plates complying the SBS standard. For using PCR plates our SmartBlocks PCR 96 and PCR 384 in combination with ThermoMixer C is the proper choice.
The ThermoMixer / ThermoStat shows the error message “ThermoTop not recognized”.
Please check if a compatible SmartBlock has been attached and the ThermoTop has been vertically placed from above. The centering pins behind the heating/cooling plate have to fit perfectly into the recesses of the ThermoTop. Place the ThermoTop once again upon the device. In case the device shows still the error message, please contact the technical service.
Do the IsoRacks fit upon the SmartBlocks for microreaction tubes (0.5 mL; 1.5 mL; 2.0 mL)?
No. The IsoRacks fit only upon the Thermoblocks for the older devices Thermomixer comfort, Thermomixer compact & ThermoStat plus.
Can PCR plates be used in SmartBlock plates respectively ThermoMixer FP?
SmartBlock plates and ThermoMixer FP (with the fixed SmartBlock plates) are dedicated for using MTP’s, DWP’s and also other skirted plates which meet the SBS standard. For optimal usage of PCR plates the SmartBlock PCR 96 and PCR 384 in combination with ThermoMixer C or ThermoStat C is the proper choice. These SmartBlocks possess conical bores which surround the conical wells of PCR plates properly for a perfect temperature transition.
Can the ThermoStat C be used in a cold room?
Yes, one can use the devices in a cold room but some safety hints should be attended: Condensate can form in the device after it has been moved from a cool environment to a warmer environment. Wait at least 3 hours after positioning the device. Plug the device only after this time has elapsed.
What does the term 2DMix-Control technology describe/stand for?
2DMix-Control technology describes the ability of the MixMate, ThermoMixer C and ThermoMixers F to mix small sample volumes in various plate and tube formats quickly and in a controlled manner. To achieve this, the interplay of all crucial factors for efficient and controlled mixing, have been optimized. And these are:
- Generation of sufficient mixing energy: due to an orbital mixing movement at a high mixing speed and a relatively large mixing radius (1.5 mm) the devices generate enough mixing energy (analogous to g-force in centrifuges) to mix even the smallest volumes efficiently.
- Minimized loss of mixing energy: special attention was paid to reducing inherent vibration. Smooth operation combined with reliable stability ensures that all the mixing energy is available for mixing and not wasted through transmission of vibrations to the device or its surroundings.
- Controlled transmission of energy from the device to the sample: Minimizing vertical movements enable a plane, 2-dimensional (2D) mixing stroke. This, in conjunction with the reliable plate and tube holders and SmartBlocks, prevents uncontrolled chaotic movements and allows all formats to be held securely, even at high mixing frequencies.
What types of applications can be carried out with Eppendorf Tubes® 5.0 mL?
Basically, this tube type can be used for the same applications as the micro centrifugation tubes available in 1.5 and 2 mL formats. They close a gap between this small tube format and conical 15 mL tubes for sample volumes up to 5 mL. The tubes can be used in the field of cell culture. For this purpose, they are available in the purity grades “Sterile” (= sterile and pyrogen-free) or Biopur®. They are suitable for lysis of cells and tissues and purification of nucleic acids and proteins. For this, the PCR clean purity grade and LoBind variants are provided. They are also ideally suited for the preparation of large volumes of master mixes, buffers or dilution series as well as for space-saving storage of samples.
Is it necessary to buy new equipment to be able to use the new tube?
The bottom shape of the Eppendorf Tubes® 5.0 mL is similar to that of conical 15 mL tubes which means that they are compatible with a wide range of standard laboratory equipment. In addition, an extensive range of accessories is available, enabling them to be used in many devices. Both racks and storage boxes are available for lab bench work and storage. Incubation and mixing operations can be performed using suitable blocks on Eppendorf thermomixers and compatibility with Eppendorf centrifuges is ensured by special rotors and a variety of adapters. With a suitable reservoir rack module, the tubes can also be integrated into the epMotion® automated liquid handling workstation. A detailed description of the accessories is provided in Application Note No. 265
Is it possible to store samples in 5.0 mL Eppendorf Tubes® in the freezer?
Yes, the tubes can be stored down to -86 °C.
How resistant are the Eppendorf Tubes® 5.0 mL to chemicals?
The tubes are made of high quality highly transparent polypropylene and therefore resistant to a variety of commonly used chemical substances in the laboratory. An overview is provided in Application Note No. 56.
Are the Eppendorf Tubes® 5.0 mL available with the same purities and LoBind variants as the small micro centrifugation tubes from Eppendorf?
The Eppendorf Tubes® 5.0 mL are available in the purity grades Eppendorf Quality, PCR clean and Biopur® and also in the purity grade Sterile. In addition to irradiation for sterilization, this also includes certified testing for the absence of pyrogens. The tubes are also available as DNA LoBind and Protein LoBind variants.
Can the universal adapter for conical 15 mL screw-cap tubes be used in each rotor/adapter that is designed for this tube type?
It can be used in the respective Eppendorf rotors/adapters, but we assume no liability for use in rotors/adapters from other manufacturers.
What is the Tube Clip used for?
The Eppendorf Tubes® 5.0 mL offer secure closure at incubation temperatures up to 80 °C. For higher temperatures, the Tube Clip should be used to secure the lid.
Up to which g-force can the Eppendorf Tubes® 5.0 mL be centrifuged?
All tube types are resistant up to 25,000 x g when used in combination with a suitable rotor or adapter. (Test conditions: 45° fixed-angle rotor at 40 °C, with aqueous salt solution (density 1.0 g/mL) and 90 mins). The mechanical loading capacity of the tubes is reduced by the use of organic solvents.
How do Cell Culture Consumables enable gas exchange?
In Cell Culture Consumables gas exchange occurs via the vented lid design for plates or dishes or the integrated filter in filter cap flask. The Eppendorf plug-seal cap is equipped with a defined arrested and venting position that prevents unintended full closing.
How can I calculate the correct rotor speed (RPM) to use for pelleting my cells by centrifugation?
Most common protocols refer to 300 x g for 5 min to pellet eukaryotic cells. For information related to Eppendorf centrifuges and rotors, please refer to: Eppendorf centrifuges
Can I store my cell culture products in the biosafety cabinet during UV-Sterilization?
Eppendorf Cell Culture products are not resistant to longer UV-radiation with an intensity that is usually used for UV-sterilization of biosafety cabinets.
What is the temperature range the Eppendorf Cell Culture Consumables can be used?
Eppendorf Cell Culture Consumables are stable in the range of -86°C to 60°C.
Do the Eppendorf Cell Culture Consumables promote the attachment and proliferation of adherent cells?
We offer tissue culture (TC) treated or non-treated cell culture consumables. The TC treated products promote adhesion of adherent cells on the surface. The TC treatment procedure generates charged groups on the usually hydrophobic polystyrene surface. This facilitates cell attachment and spreading of most anchorage dependent cells.
What can be the cause for poor cell attachment?
Multiple factors can influence cell attachment or growth. For instance serum-reduced or serum-free conditions or transfection of cells can result in poor cell attachment. Some cell types require a surface with biological coatings like fibronectin, poly-D/L-lysine, collagen etc.
What is the chemical resistance of Eppendorf Cell Culture Products?
Please contact your local Eppendorf Application Support for information on chemical resistance.
Are the Eppendorf Cell Culture Flasks certified leakage proofed?
Yes, a 100% in-line pressure test for each flask guarantees leakage-free flasks for maximum workflow safety.
What filter technology does Eppendorf use for the filter cap and what benefits does this technology offer?
The Eppendorf filter cap is equipped with a high efficiency hydrophobic air filter. This new filter technology combines improved protection with reliable gas exchange. Due to its hydrophobicity the filter repels liquid splashes effectively.
How do I use the black bend-seal on the dish package?
All Eppendorf Cell Culture Consumable multipack packages are resealable to provide maximum product safety. The packaging of the dishes has – in addition to the sticky sides for resealing - a bend seal for extra safety and space saving storage.
What is the purpose of the ring in the lid of the dish?
The SplashProtect™ ring inside the dish lid traps liquid drops which may occur from spillages during transportation. This reduces the risk of cross-contaminations and gives an extra security during all handling and transport of dishes.
Why is the 96-well plate equipped with an outer moat?
The outer moat of the 96-well plate provides the option to reduce the edge effect: It can be easily filled with liquid (5 mL) to reduce evaporation. Find more information about reducing the edge effect in Application Note No.326.
Can I use Eppendorf Cell Culture Plates for automated systems?
The Eppendorf Cell Culture Plates conform to the SBS standard. Therefore they fit to most automated systems.
Does the SplashProtect™ ring inside the dish lid impairs the gas exchange?
Gas exchange is facilitated by the vented lid design. The SplashProtect™ ring inside the lid has no influence on the gas exchange.
Where do I find the order number and the lot number?
You can find the order number as well as the lot number on the primary sleeve packaging as well as on the outer box packaging. In addition, you will find the lot number on each Eppendorf Cell Culture Flask.