Seminar Details

Microinjection in Zebrafish and Medaka: From Transgenesis to CRISPR

Target group:

Research technicians and scientists interested in the generation of genetic modified fish models. Basic experience with molecular biology techniques is required.

Objective of seminar:

The aim of the course is to provide participants with hands-on tutorials and the theory behind microinjection into medaka and zebrafish. In addition, participants will learn to design CRISPR constructs.

Content of seminar:
  • Microinjection of lineage tracers into one-cell stage embryos
  • Co-injection of DNA and mRNA into one-cell stage embryos
  • Microinjection of CRISPR/Cas constructs
  • Targeted inactivation of pigment loci or genes of interest provided by applicants (medaka and zebrafish)
  • Homology directed repair in fish
  • Screening by imaging
  • High throughput, high content screening for successful events
  • Molecular validation of Crispr/Cas9 mediated indel formation

450 Euro (Academia and PhD Student), 800 Euro Industry

Starting date:
2019-07-02 - 9:00 a.m.

Finish date:
2019-07-04 - 04:00 p.m.

Net price:
€ 450,00 (Academia and PhD Sutdent), € 800 (Industry) excl. VAT

S. Stobrawa, J. Wittbrodt, E. Zielonka, T. Thumberger

Max attendees:
max. 8 participants


Heidelberg, Germany (In cooperation with EMBL)

This course will be held at the ATC in close cooperation with the EMBL in Heidelberg. It is possible to bring your own sample material; please contact the trainer in advance.

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