NGS methods for epMotion® 5073m NGS and 5075t NGS

The epMotion can be equipped with an on-deck thermal mixer and a thermal module and is proven to be the most efficient configuration at enabling automated NGS library preparation. With such configuration, a list of NGS library preparation workflows have been automated with the latest epBlue software, then checked and approved by reagent manufacturers. All methods are programmed according to manufacturer’s user guides.

Automated epMotion methods for the following kits are qualified by reagent manufacturers:

epMotion® 5073m NGS solution

Illumina® 16S Metagenomic

Illumina Nextera DNA Flex

Illumina TruSeq® FFPE DNA QC Library Prep

Illumina Nextera XT

Illumina TruSeq DNA Nano

KAPA® HyperPlus

KAPA Library Quant Kit (LQK)

AmpliSeq® for Illumina Myeloid

AmpliSeq for Illumina BRCA Panel

AmpliSeq for Illumina HotSpot

AmpliSeq for Illumina Comprehensive Cancer Panel

AmpliSeq for Illumina Focus Panel

Agilent® Technologies Haloplex®

Agilent Genomics – qPCR NGS Library Quantification Kit

Multiplicom/Agilent Technologies – BRCA Hereditary Cancer

MASTR Plus

NEBNext® Library Quant Kit for Illumina

QuantaBio® – PerfeCTa NGS Quantification Kit

Qiagen® Generead targeted DNA/RNA panel

QIASeq® FX

Takara – NGS library quantification kit

 

epMotion® 5057t NGS solution

Ampliseq for Illumina®: Cancer HotSpot Panel v2

Ampliseq for Illumina®: Focus Panel

Ampliseq for Illumina®: Comprehensive Panel Panel v3

Illumina® TruSeq® Stranded Total RNA (RiboZero)

Illumina® TruSeq® Stranded mRNA

Illumina® TruSeq® Nano DNA

Illumina® TruSeq® DNA PCR-Free

Illumina® TruSeq® Rapid Exome

Illumina® TruSeq® RNA Exome

Illumina® TruSeq® Custom Amplicon Low Input

Illumina® TruSight® HLA v2

Illumina® TruSight® Tumor 15

Illumina® TruSight® RNA Pan Cancer

Illumina® TruSight® RNA Fusion

Illumina® TruSight® Cancer/TruSight® Enrichment

Illumina® TruSight® Rapid Capture

Illumina® TruSight® One

Illumina® Nextera® XT DNA

Illumina® Nextera® Rapid Capture Custom Enrichment

Illumina® Nextera® DNA Flex

Illumina® Nextera® Flex for Enrichment

KAPA® HyperPrep

KAPA® mRNA HyperPrep

KAPA® HyperPlus

KAPA® HTP LPK

KAPA® Stranded RNA Seq

KAPA® Library Quant

 

The following kits have been integrated successfully in customers' workflows with the assistance of our automation experts. Many were modified and optimized to work with the individual situation, such as the epMotion configuration, available accessories and consumable choices:

Illumina® Nextera® Rapid Capture Exome

Illumina® ForenSeq™

KAPA® RNA HyperPrep

KAPA® mRNA Seq

KAPA® Ribo Erase

Agilent® HaloPlex®

Agilent® SureSelect® XT

New England Biolabs® NEBNext® Ultra

New England Biolabs® NEBNext® Ultra II

Qiagen® QIAseq FX

Qiagen® GeneRead™ Panel

 

Further NGS methods are in preparation.

 

Disclaimer: Methods are intended for molecular research applications. They are not intended, verified or validated for use in the diagnosis of disease or other human health conditions.

Contact Eppendorf: www.eppendorf.com/contact

 

Illumina® is a leading provider of next-generation sequencing technology used for the analysis of genetic variation and function. Our portfolio of sequencing systems offers a range of options for diverse study designs—from large-scale analyses involving numerous whole genomes, to highly targeted analyses with smaller sample volumes. Illumina® systems are built on proven sequencing by synthesis (SBS) technology, which delivers incredibly accurate data and offers the most streamlined user workflow. To enable a seamless user experience, Illumina® also develops sample preparation kits and bioinformatic solutions that are optimized for the sequencing workflow. Our Nextera and TruSeq sample preparation technologies allow researchers to quickly and easily generate sequencing-ready libraries for virtually any genomic, transcriptomic, or epigenomic analysis. Contact Illumina®