Why does the vortexer continue to run for approx. 2 seconds after relieving pressure on the vortex mat?
This 2-second overrun makes the vortexing process more ergonomic, because the vortex mat need not be pressed continuously to keep the vortexer running. It also eases the process of performing vortexing in several tubes successively.
My plate does not fit into the Universal Holder, why?
The Universal Holder can only accommodate plate formats that comply with the SBS Standard for Microplates
Is the housing of ThermoMixer C, ThermoMixer F and ThermoStat C stable against UV irradiation?
A disinfection by UV irradiation cannot be recommended, because discoloration of the housing materials can occur.
Which DNA and RNAse deconatmination agents can be recommended for decontamination of the devices and the SmartBlocks?
We tested DNA away and RNase away and we can recommend to use both agents.
Are start and end position of the SmartBlocks the same?
No. Start and end position are not identical.
How can the temperature accuracy be verified?
The best possible temperature accuracy can be guaranteed by individually adjusting a SmartBlock. Optimized temperature control for each block type keeps temperature overshoots and deviations caused by external interferences to a minimum. To guarantee optimal performance over the entire period of use, Eppendorf offers a preventive maintenance program and certification services: your Eppendorf ThermoMixers, ThermoStat C as well as the SmartBlocks are regularly cleaned, checked and adjusted. Additional information is available at: https://www.eppendorf.com/epservices
. Customers can also verify the temperature accuracy by themself. Eppendorf provides for that purpose the Temperature Verification System – Single Well for Eppendorf Mastercyclers (0056 000.003) in combination with the 384 well Sensor (0056 002.006).
Can cell culture plates be used in the SmartBlock plates?
Also cell cultuture plates can be used in the SmartBlock plates in so far the dimensions of the plate comply to the SBS standard.
Is it possible to put the SmartBlocks for a rapid cool down into a -20°C freezer?
The SmartBlocks can be used at ambient temperatures of 5°C – 40°C and can be cooled appropriate. Cooling down of SmartBlocks in a freezer should not be done.
Is it possible to run more than one program in automatic succession on a ThermoMixer or ThermoStat C?
No. Each program has to be chosen and run separately. But the 4 possible steps within a program run in automatic succession.
Which SmartBlocks and vessels can be used with ThermoMixers F?
The ThermoMixers F possess a fix installed SmartBlock and can only be used with the appropriate microtest tubes or plates, such as MTP’s, DWP’s or skirted plates complying the SBS standard. For using PCR plates our SmartBlocks PCR 96 and PCR 384 in combination with ThermoMixer C is the proper choice.
Do the IsoRacks fit upon the SmartBlocks for microreaction tubes (0.5 mL; 1.5 mL; 2.0 mL)?
No. The IsoRacks fit only upon the Thermoblocks for the older devices Thermomixer comfort, Thermomixer compact & ThermoStat plus.
Can PCR plates be used in SmartBlock plates respectively ThermoMixer FP?
SmartBlock plates and ThermoMixer FP (with the fixed SmartBlock plates) are dedicated for using MTP’s, DWP’s and also other skirted plates which meet the SBS standard. For optimal usage of PCR plates the SmartBlock PCR 96 and PCR 384 in combination with ThermoMixer C or ThermoStat C is the proper choice. These SmartBlocks possess conical bores which surround the conical wells of PCR plates properly for a perfect temperature transition.
What does the term 2DMix-Control technology describe/stand for?
2DMix-Control technology describes the ability of the MixMate, ThermoMixer C and ThermoMixers F to mix small sample volumes in various plate and tube formats quickly and in a controlled manner. To achieve this, the interplay of all crucial factors for efficient and controlled mixing, have been optimized. And these are:
- Generation of sufficient mixing energy: due to an orbital mixing movement at a high mixing speed and a relatively large mixing radius (1.5 mm) the devices generate enough mixing energy (analogous to g-force in centrifuges) to mix even the smallest volumes efficiently.
- Minimized loss of mixing energy: special attention was paid to reducing inherent vibration. Smooth operation combined with reliable stability ensures that all the mixing energy is available for mixing and not wasted through transmission of vibrations to the device or its surroundings.
- Controlled transmission of energy from the device to the sample: Minimizing vertical movements enable a plane, 2-dimensional (2D) mixing stroke. This, in conjunction with the reliable plate and tube holders and SmartBlocks, prevents uncontrolled chaotic movements and allows all formats to be held securely, even at high mixing frequencies.
Is it possible to use the Eppendorf Thermomixer for 24 h and at 7 days?
Yes, this is possible. The customer has just to set the time to endless.
What types of applications can be carried out with Eppendorf Tubes® 5.0 mL?
Basically, this tube type can be used for the same applications as the micro centrifugation tubes available in 1.5 and 2 mL formats. They close a gap between this small tube format and conical 15 mL tubes for sample volumes up to 5 mL. The tubes can be used in the field of cell culture. For this purpose, they are available in the purity grades “Sterile” (= sterile and pyrogen-free) or Biopur®. They are suitable for lysis of cells and tissues and purification of nucleic acids and proteins. For this, the PCR clean purity grade and LoBind variants are provided. They are also ideally suited for the preparation of large volumes of master mixes, buffers or dilution series as well as for space-saving storage of samples.
Is it necessary to buy new equipment to be able to use the new tube?
The bottom shape of the Eppendorf Tubes® 5.0 mL is similar to that of conical 15 mL tubes which means that they are compatible with a wide range of standard laboratory equipment. In addition, an extensive range of accessories is available, enabling them to be used in many devices. Both racks and storage boxes are available for lab bench work and storage. Incubation and mixing operations can be performed using suitable blocks on Eppendorf thermomixers and compatibility with Eppendorf centrifuges is ensured by special rotors and a variety of adapters. With a suitable reservoir rack module, the tubes can also be integrated into the epMotion® automated liquid handling workstation. A detailed description of the accessories is provided in Application Note No. 265
Is it possible to store samples in 5.0 mL Eppendorf Tubes® in the freezer?
Yes, the tubes can be stored down to -86 °C.
How resistant are the Eppendorf Tubes® 5.0 mL to chemicals?
The tubes are made of high quality highly transparent polypropylene and therefore resistant to a variety of commonly used chemical substances in the laboratory. An overview is provided in Application Note No. 56.
Are the Eppendorf Tubes® 5.0 mL available with the same purities and LoBind variants as the small micro centrifugation tubes from Eppendorf?
The Eppendorf Tubes® 5.0 mL are available in the purity grades Eppendorf Quality, PCR clean and Biopur® and also in the purity grade Sterile. In addition to irradiation for sterilization, this also includes certified testing for the absence of pyrogens. The tubes are also available as DNA LoBind and Protein LoBind variants.
Can the universal adapter for conical 15 mL screw-cap tubes be used in each rotor/adapter that is designed for this tube type?
It can be used in the respective Eppendorf rotors/adapters, but we assume no liability for use in rotors/adapters from other manufacturers.
What is the Tube Clip used for?
The Eppendorf Tubes® 5.0 mL offer secure closure at incubation temperatures up to 80 °C. For higher temperatures, the Tube Clip should be used to secure the lid.
Up to which g-force can the Eppendorf Tubes® 5.0 mL be centrifuged?
All tube types are resistant up to 25,000 x g when used in combination with a suitable rotor or adapter. (Test conditions: 45° fixed-angle rotor at 40 °C, with aqueous salt solution (density 1.0 g/mL) and 90 mins). The mechanical loading capacity of the tubes is reduced by the use of organic solvents.
For which applications may I use Eppendorf Conical Tubes® 25 mL?
In general, all applications where standard conical tubes 15mL and 50 mL are routinely used. The Eppendorf Conical Tubes® 25 mL close a gap between 15 mL and 50 mL conical tubes and additionally offer in this format a unique snap cap – the so called SnapTec™ cap - which markedly improves handling. This makes the Eppendorf Conical Tubes® 25 mL ideally suited for all cell/tissue culture applications including splitting cells, media change, transfections, etc. Also assay preparation and biomolecule purification protocols (gDNA, plasmid DNA, RNA, proteins), which require organic solvent extractions and multiple centrifugation steps may be successfully performed. They are also ideally suited for general reagent / sample handling and space-saving storage.
Is it necessary to buy new equipment to enable the use of Eppendorf Conical Tubes 25 mL within existing lab periphery?
No. The bottom shape / diameter of the Eppendorf Conical Tubes 25 mL is virtually the same with standard conical 50 mL tubes. With the respective height adapter (provided as Eppendorf accessory), the Eppendorf Conical Tubes 25 mL will be compatible with a wide range of standard laboratory equipment: centrifuges, mixers, etc., additionally tube racks, storage boxes and single-tube stands (skirts) are available for lab bench work, weighing and storage. For detailed accessories description please refer to www.Eppendorf.com .
Is it possible to store samples in 25 mL Eppendorf Conical Tubes in the freezer?
Yes, the tubes can be stored down to -86 °C.
How resistant are the Eppendorf Conical Tubes® 25 mL to chemicals?
The tubes are made of high quality, transparent polypropylene and resistant to a majority of commonly used chemical substances in the laboratory. An overview is provided in Application Note No. 56. In case a substance/chemical is not listed there, please contact our local contact.
Which purity grades and LoBind / Amber variants are available for Eppendorf Conical Tubes 25 mL?
The Eppendorf Conical Tubes 25 mL are available in the purity grades: Eppendorf Quality, PCR clean and Sterile/Pyrogen-free. For general and lot-specific certificates please refer to Eppendorf website eppendorf.com/certificates. The DNA and Protein LoBind as well as Amber variants of Eppendorf Conical Tubes® 25 mL will be available within short term as well.
Correct opening and closing of the snap Lid (SnapTec®).
The snap cap of the Eppendorf Conical Tubes® 25 mL is much larger than in other tube formats and may require some short learning of correct closing/opening at the beginning. Opening: press the safety tab from below and steady press upwards. In case of pressure buildup (organic solvents, higher temp incubations) make sure you slightly hold the lid with your thumb and don’t let the lid to pop out rapidly. Closing: press down the lid in the front area and make sure the safety tab clicks and is locked at right angle to the tube. Improperly locked tab may cause leaking.
Smart Block cryo Thaw on ThermoMixer C - What is the ‘Thawing cells’ program?
The 'Thawing cells' program is an optimized program to thaw mammalian cells in 1.8-2.0 mL cryovials:
It is a ready-to use program, in most cases no changes are required. The pre-set time is 3 minutes (can be extended up to 5 minutes), the pre-set mixing frequency is 500 rpm (can be adapted between 300 – 800 rpm), the temperature profile is fixed.
The settings are based on tests with standard mammalian cell lines (CHO, HEK, S16) up to highly sensitive stem cells (hiPSCs) at tested conditions with 2.0 mL cryovials / 1.0 mL fill (1 – 5 vials in parallel).
Other volumes or more vials may require an adaption of thawing time.
Further information can be found in the Application Note 437
What is the difference between the SmartBlock cryo thaw and the SmartBlock cryo?
The 'SmartBlock cryo thaw' replaces the 'SmartBlock cryo'. Newly implemented is an automatic function for thawing cells (program 'Thawing cells') which is available in combination with the ThermoMixer C.