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793 Dokumenty
Do you know the order number for the solenoid valve with special plug for connecting a…
Do you know the order number for the solenoid valve with special plug for connecting a vacuum pump to Concentrator plus basic device?
The Order numbers for solenoid valve (incl. special plug) are:
for 230 V connection: 5301 030.004
for 115 V connection: 5301 036.002
When is it possible to open the Lid?
When is it possible to open the Lid?
When the device is switched on, the lid can be opened as long as the control lamp Lid lights up.
How do I open the lid in case of a power failure?
How do I open the lid in case of a power failure?
In the event of power failure, the ventilation valve will automatically open and the chamber will aerate to atmospheric pressure before the rotor stops spinning. Locate the pinhole on the right side of the housing, near the top rear corner. Insert a paper clip and the lid will open.
Is the solenoid valve for the control of an external pump with the basic device chemical…
Is the solenoid valve for the control of an external pump with the basic device chemical resistant?
The solenoid valve for control of an external pump is resistant against water and alcohols.
Can the new coated lid be used with the existing Concentrator Plus?
Can the new coated lid be used with the existing Concentrator Plus?
Yes, the new lid is fully backward compatible
How is the Centrifuge 59xx protected during transport? Is there a transport protection…
How is the Centrifuge 59xx protected during transport? Is there a transport protection device?
No, the Centrifuge 59xx does not come with a transport protection device. It is packaged in polystyrene for protection. Because the rotor is not in the centrifuge during transport, there are no movable parts to make a transport protection device necessary.
What is the UVette made of?
What is the UVette made of?
The UVette is made of a UV-transparent plastic.
Can the UVette be used in spectrophotometers of other manufacturers?
Can the UVette be used in spectrophotometers of other manufacturers?
In general this is possible by using an adapter which we offer for light beam heights of 8.5, 10, 15 and 20 mm. Choose the adapter according to the height of the measuring beam in your spectrometer. Please contact therefore the manufacturer of your spectrometer directly or please ask your local Eppendorf office for assistance.
Can I reuse the UVette?
Can I reuse the UVette?
We recommend that the UVette be discarded following the sample measurement in order to guarantee contamination-free analysis. Cleaning processes and/or multiple use of an UVette may damage the quality of the optical surfaces, particularly in the sensitive UV-range. However, the UVette may be used up to five times for UV measurements if the Uvette material is not damaged during the cleaning procedures.
How to measure in the right way with the UVette?
How to measure in the right way with the UVette?
As with quartz glass cuvettes, it is important to make certain that the UVette is placed in the same direction in the cuvette shaft for both the blank and the sample measurement. Because the self-absorbance values of the UVettes vary somewhat from one another, ideally, the same UVette should be used for both measurements. If changing the UVette when measuring a series of samples (recommendation: up to five measurements with one UVette) also the blank should be repeated using the new UVette.
Is it possible with the Eppendorf BioSpectrometer that within one method several…
Is it possible with the Eppendorf BioSpectrometer that within one method several calibration curves can be stored?
Only the last used standard curve of one method can be used. This one can be selected by the function "Last cal"
In what file-format are the files from the BioSpectrometer and BioPhotometer D30 exported? …
In what file-format are the files from the BioSpectrometer and BioPhotometer D30 exported?
Data can be exported as Excel-files on a USB-Stick or directly to a PC. There is no recommendation for specific USB-Stick. The only requirement is a FAT32 format. It is not required to export the complete data set but there is the possibility to select results and the results output. Additionally, it is planned in a device update for 2016 that data alternatively can be exported as pdf. Also direct printing of data via a network printer as well as data transfer via email will be enable if the device is connected via LAN cable to the home network.
Can the reagents for the Qubit 2.0 be used in the BioSpectrometer fluorescence?
Can the reagents for the Qubit 2.0 be used in the BioSpectrometer fluorescence?
The following Qubit reagents for the detection of dsDNA can be used: Qubit dsDNA HS and Qubit dsDNA BR. Moreover we offer a preprogrammed method for the detection of ssDNA (Qubit ssDNA). Please note, that for all Qubit methods 4 standards have to be measured. All standard concentrations that have to be measured are preprogrammed.
What kind of molecules can be detected via fluorimetry in the BioSpectrometer fluorescence …
What kind of molecules can be detected via fluorimetry in the BioSpectrometer fluorescence
The BioSpectrometer fluorescence has preprogrammed methods for fluorimetric detection of dsDNA, ssDNA, oligos, RNA and proteins.
What do I have to consider for cleaning the Eppendorf µCuvette?
What do I have to consider for cleaning the Eppendorf µCuvette?
Always use for cleaning lint-free tissue papers. Do not use therefore any chemicals that are not recommended in the operation manual. Particularly substances that tend to corrosion such as acids or bases should be avoided. Nucleic acids could be removed easily with water. But it is also possible to clean the cuvette with a 6% sodium hypochlorite solution to remove persistent nucleic acid contaminations. A cleaning in a ultrasonic bath is not recommended
After adding the sample to the Eppendorf µCuvette no liquid column could be formed. What…
After adding the sample to the Eppendorf µCuvette no liquid column could be formed. What could be the reason for this?
One reason can be, that the volume of the used sample was simply too low.
Another possibility is that after purification of DNA samples it may appear that there is still high amount of protein inside the sample. This may lead to a reduced surfaced tension of the liquid. This problem could be solved by slightly increasing the measuring volume. If solutions are containing detergent, it may appear that liquid column cannot be formed at all. In this case the sample should be measured in a cuvette (e.g. Eppendorf UVette).
In which spectrophotometer can the Eppendorf µCuvette be applied?
In which spectrophotometer can the Eppendorf µCuvette be applied?
The Eppendorf µCuvette can be used for all Eppendorf BioSpectrometer and the BioPhotometer D30, 6131 und 6132. It can not be applied spectrophotometer of other manufacturers.
What wavelength could be applied in the BioPhotometer D30?
What wavelength could be applied in the BioPhotometer D30?
The wavelengths 230, 260, 280, 320, 340, 405, 490, 562, 595 und 600 nm could be applied.
What can I do with the so called "Purity scan"?
What can I do with the so called "Purity scan"?
With the "purity scan" the absorbance of a certain wavelength range is shown for nucleic acid and protein measurements. With this function impurities in the sample can be easily detected via deviations from the known curve. For protein measurements it is the wavelength range from 245-350 nm and for nucleic acids the range from 225-350nm. Only the curve shape will be indicated whereas single measurement values cannot be shown.
In what file-format are the files from the BioSpectrometer and BioPhotometer D30 exported? …
In what file-format are the files from the BioSpectrometer and BioPhotometer D30 exported?
Data can be exported as Excel-files on a USB-Stick or directly to a PC. There is no recommendation for specific USB-Stick. The only requirement is a FAT32 format. It is not required to export the complete data set. There is the possibility to select results and the results output.
How many methods and results can be stored in the BioSpectrometer and BioPhotometer D30? …
How many methods and results can be stored in the BioSpectrometer and BioPhotometer D30?
About 100 methods and 1000 results can be stored in the BioPhotometer D30 or the BioSpectrometer. One result can be consist of 99 measurements.
What kind of application could be carried out with the Eppendorf Vis Cuvettes?
What kind of application could be carried out with the Eppendorf Vis Cuvettes?
All photometric detection methods with measuring wavelengths in the visible range of light could be applied, e.g. colorimetric protein detection, measuring of optical densities of bacterial cultures, enzyme kinetics
What material are Eppendorf Vis Cuvettes are made of?
What material are Eppendorf Vis Cuvettes are made of?
The Eppendorf Vis Cuvettes are made of PMMA
In what wavelength range of light are the Eppendorf cuvettes are transparent?
In what wavelength range of light are the Eppendorf cuvettes are transparent?
The Eppendorf Vis Cuvettes are transparent for light in the visible range from 300 to 900 nm
Are the Eppendorf Vis Cuvettes suitable for kinetic measurements?
Are the Eppendorf Vis Cuvettes suitable for kinetic measurements?
If anexact temperature should be maintained during the measurement, we recommend to use the Eppendorf macro Vis cuvettes.
Are there any chemical resistance lists for the use of Eppendorf VIS cuvettes?
Are there any chemical resistance lists for the use of Eppendorf VIS cuvettes?
Please consider therefore our description in the operation manual in chapter 5.3. Moreover you could check any chemical resistance table for PMMA!
What material is the cuvette stand made of and can it be autoclaved?
What material is the cuvette stand made of and can it be autoclaved?
The cuvette stand for electroporation cuvettes is made of polypropylene and can be autoclaved at 121 °C.
Are there protocols for the electroporation of filamentous fungi using the Eporator or the…
Are there protocols for the electroporation of filamentous fungi using the Eporator or the Multiporator?
Yes, there is a protocol available for Neurospora crassa (Filamentous fungus, conidia) for the Eporator or the Multiporator (incl. prokaryotic module).
Is a special buffer required for the electroporation of bacteria?
Is a special buffer required for the electroporation of bacteria?
No special buffer is required. Distilled water is frequently used, otherwise a standard buffer depending on the protocol can be used. Care has to be taken that it has a low conductivity.
Do you have protocols for the electroporation of plants using the Eppendorf Eporator or…
Do you have protocols for the electroporation of plants using the Eppendorf Eporator or Multiporator?
Yes, there is a protocol available for Arabidopsis thaliana (Protocol No. 4308 915 548-05-2012) and for Citrullus colocynthis (protoplasts, Protocol no. 4308 915 549-05-2012)