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1197 Dokumenty
Where do I find the order number and the lot number?
Where do I find the order number and the lot number?
You can find the order number as well as the lot number on the label of the aluminum-based packaging as well as on the outer box packaging.
Are your products certified sterile, free from Endotoxins and DNA, DNase and RNase?
Are your products certified sterile, free from Endotoxins and DNA, DNase and RNase?
Yes, all Eppendorf Cell Culture Consumables are certified non-cytotoxic (absence of pyrogens) and free of human and bacterial DNA, DNase and RNase. For detailed information please refer to the respective certificate of quality and the lot-specific certificates.
Are your products free of BSE/TSE?
Are your products free of BSE/TSE?
Eppendorf only works with granulate suppliers who guarantee that their used animal components come exclusively from countries without BSE occurrences. Risky materials are not used. The granulate production includes process steps with conditions above 235°C/ 3,000EkPa for several hours that destroy any virus, bacteria or substance that cause immunological diseases (TSE, BSE, CJD). For detailed information please refer to the respective certificate of quality and the lot-specific certificates.
What are the recommended working volumes for the different formats?
What are the recommended working volumes for the different formats?
For information regarding working volume, maximum volume, and growth area of the different formats please refer to the Eppendorf CCCadvanced FN1 motifs user manual chapter 4/technical data.
For which applications may I use Eppendorf Conical Tubes® 25 mL?
For which applications may I use Eppendorf Conical Tubes® 25 mL?
In general, all applications where standard conical tubes 15mL and 50 mL are routinely used. The Eppendorf Conical Tubes® 25 mL close a gap between 15 mL and 50 mL conical tubes and additionally offer in this format a unique snap cap – the so called SnapTec™ cap - which markedly improves handling. This makes the Eppendorf Conical Tubes® 25 mL ideally suited for all cell/tissue culture applications including splitting cells, media change, transfections, etc. Also assay preparation and biomolecule purification protocols (gDNA, plasmid DNA, RNA, proteins), which require organic solvent extractions and multiple centrifugation steps may be successfully performed. They are also ideally suited for general reagent / sample handling and space-saving storage.
Is it necessary to buy new equipment to enable the use of Eppendorf Conical Tubes 25 mL…
Is it necessary to buy new equipment to enable the use of Eppendorf Conical Tubes 25 mL within existing lab periphery?
No. The bottom shape / diameter of the Eppendorf Conical Tubes 25 mL is virtually the same with standard conical 50 mL tubes. With the respective height adapter (provided as Eppendorf accessory), the Eppendorf Conical Tubes 25 mL will be compatible with a wide range of standard laboratory equipment: centrifuges, mixers, etc., additionally tube racks, storage boxes and single-tube stands (skirts) are available for lab bench work, weighing and storage. For detailed accessories description please refer to www.Eppendorf.com .
Is it possible to store samples in 25 mL Eppendorf Conical Tubes in the freezer?
Is it possible to store samples in 25 mL Eppendorf Conical Tubes in the freezer?
Yes, the tubes can be stored down to -86 °C.
How resistant are the Eppendorf Conical Tubes® 25 mL to chemicals?
How resistant are the Eppendorf Conical Tubes® 25 mL to chemicals?
The tubes are made of high quality, transparent polypropylene and resistant to a majority of commonly used chemical substances in the laboratory. An overview is provided in Application Note No. 56. In case a substance/chemical is not listed there, please contact our local contact.
Which purity grades and LoBind / Amber variants are available for Eppendorf Conical Tubes…
Which purity grades and LoBind / Amber variants are available for Eppendorf Conical Tubes 25 mL?
The Eppendorf Conical Tubes 25 mL are available in the purity grades: Eppendorf Quality, PCR clean and Sterile/Pyrogen-free. For general and lot-specific certificates please refer to Eppendorf website eppendorf.com/certificates. The DNA and Protein LoBind as well as Amber variants of Eppendorf Conical Tubes® 25 mL will be available within short term as well.
Correct opening and closing of the snap Lid (SnapTec®).
Correct opening and closing of the snap Lid (SnapTec®).
The snap cap of the Eppendorf Conical Tubes® 25 mL is much larger than in other tube formats and may require some short learning of correct closing/opening at the beginning. Opening: press the safety tab from below and steady press upwards. In case of pressure buildup (organic solvents, higher temp incubations) make sure you slightly hold the lid with your thumb and don’t let the lid to pop out rapidly. Closing: press down the lid in the front area and make sure the safety tab clicks and is locked at right angle to the tube. Improperly locked tab may cause leaking.
How do Cell Culture Consumables enable gas exchange?
How do Cell Culture Consumables enable gas exchange?
In Cell Culture Consumables gas exchange occurs via the vented lid design for plates or dishes or the integrated filter in filter cap flask. The Eppendorf plug-seal cap is equipped with a defined arrested and venting position that prevents unintended full closing.
How can I calculate the correct rotor speed (RPM) to use for pelleting my cells by…
How can I calculate the correct rotor speed (RPM) to use for pelleting my cells by centrifugation?
Most common protocols refer to 300 x g for 5 min to pellet eukaryotic cells. For information related to Eppendorf centrifuges and rotors, please refer to: Eppendorf centrifuges.
Can I store my cell culture products in the biosafety cabinet during UV-Sterilization? …
Can I store my cell culture products in the biosafety cabinet during UV-Sterilization?
Eppendorf Cell Culture products are not resistant to longer UV-radiation with an intensity that is usually used for UV-sterilization of biosafety cabinets.
What is the temperature range the Eppendorf Cell Culture Consumables can be used?
What is the temperature range the Eppendorf Cell Culture Consumables can be used?
Eppendorf Cell Culture Consumables are stable in the range of -86°C to 60°C.
Do the Eppendorf Cell Culture Consumables promote the attachment and proliferation of…
Do the Eppendorf Cell Culture Consumables promote the attachment and proliferation of adherent cells?
We offer tissue culture (TC) treated or non-treated cell culture consumables. The TC treated products promote adhesion of adherent cells on the surface. The TC treatment procedure generates charged groups on the usually hydrophobic polystyrene surface. This facilitates cell attachment and spreading of most anchorage dependent cells.
What can be the cause for poor cell attachment?
What can be the cause for poor cell attachment?
Multiple factors can influence cell attachment or growth. For instance serum-reduced or serum-free conditions or transfection of cells can result in poor cell attachment. Some cell types require a surface with biological coatings like fibronectin, poly-D/L-lysine, collagen etc.
What is the chemical resistance of Eppendorf Cell Culture Products?
What is the chemical resistance of Eppendorf Cell Culture Products?
Please contact your local Eppendorf Application Support for information on chemical resistance.
Are the Eppendorf Cell Culture Flasks certified leakage proofed?
Are the Eppendorf Cell Culture Flasks certified leakage proofed?
Yes, a 100% in-line pressure test for each flask guarantees leakage-free flasks for maximum workflow safety.
What filter technology does Eppendorf use for the filter cap and what benefits does this…
What filter technology does Eppendorf use for the filter cap and what benefits does this technology offer?
The Eppendorf filter cap is equipped with a high efficiency hydrophobic air filter. This new filter technology combines improved protection with reliable gas exchange. Due to its hydrophobicity the filter repels liquid splashes effectively.
How do I use the black bend-seal on the dish package?
How do I use the black bend-seal on the dish package?
All Eppendorf Cell Culture Consumable multipack packages are resealable to provide maximum product safety. The packaging of the dishes has – in addition to the sticky sides for resealing - a bend seal for extra safety and space saving storage.
What is the purpose of the ring in the lid of the dish?
What is the purpose of the ring in the lid of the dish?
The SplashProtect™ ring inside the dish lid traps liquid drops which may occur from spillages during transportation. This reduces the risk of cross-contaminations and gives an extra security during all handling and transport of dishes.
Why is the 96-well plate equipped with an outer moat?
Why is the 96-well plate equipped with an outer moat?
The outer moat of the 96-well plate provides the option to reduce the edge effect: It can be easily filled with liquid (5 mL) to reduce evaporation. Find more information about reducing the edge effect in Application Note No.326.
Can I use Eppendorf Cell Culture Plates for automated systems?
Can I use Eppendorf Cell Culture Plates for automated systems?
The Eppendorf Cell Culture Plates conform to the SBS standard. Therefore they fit to most automated systems.
Does the SplashProtect™ ring inside the dish lid impairs the gas exchange?
Does the SplashProtect™ ring inside the dish lid impairs the gas exchange?
Gas exchange is facilitated by the vented lid design. The SplashProtect™ ring inside the lid has no influence on the gas exchange.
Where do I find the order number and the lot number?
Where do I find the order number and the lot number?
You can find the order number as well as the lot number on the primary sleeve packaging as well as on the outer box packaging. In addition, you will find the lot number on each Eppendorf Cell Culture Flask.
Can any of the Eppendorf Cell Culture Consumables be reused?
Can any of the Eppendorf Cell Culture Consumables be reused?
We do not recommend
reusing the Eppendorf Cell Culture Consumables regardless of the format. All products are
intended for single use only.
Are your products certified sterile, free from Endotoxins and DNA, DNase and RNase?
Are your products certified sterile, free from Endotoxins and DNA, DNase and RNase?
Yes, all Eppendorf Cell Culture Consumables are certified non-cytotoxic (absence of
pyrogens) and free of human and bacterial DNA, DNase and RNase. Sterilization is performed
by irradiation according to DIN EN ISO 11137-2:2007 or ethylene oxide sterilization with a SAL
(sterility assurance level) of 10-6. For detailed information please refer to the respective
certificate of quality and the Lot-specific certificates.
What are the recommended working volumes for the different formats?
What are the recommended working volumes for the different formats?
For information regarding working volume, maximum volume, and growth area of the different formats
please refer to the Eppendorf Cell Culture Consumables user manual chapter 4/technical data.
Are your products free of BSE/TSE?
Are your products free of BSE/TSE?
Eppendorf only works with granulate suppliers who
guarantee that their used animal components come exclusively from countries without BSE
occurrences. Risky materials are not used. The granulate production includes process steps
with conditions above 235°C/ 3,000EkPa for several hours that destroy any virus, bacteria or
substance that cause immunological diseases (TSE, BSE, CJD). For detailed information
please refer to the respective certificate of quality and the Lot-specific certificates.
What is the temperature range the Eppendorf Cell Imaging Consumables can be used?
What is the temperature range the Eppendorf Cell Imaging Consumables can be used?
Eppendorf Cell Imaging Consumables are resistant over a wide range of temperatures (see table here), temperature shifts will not distort or harm the material. Chamber and lid of Eppendorf Cell Imaging Slides and Coverglasses are made of a highly temperature resistant Polymer and are therefore also best suited for applications requiring high temperatures, e.g. in-situ hybridization.