How can I calculate the correct rotor speed (RPM) to use for pelleting my cells by centrifugation?
Most common protocols refer to 300 x g for 5 min to pellet eukaryotic cells. For information related to Eppendorf centrifuges and rotors, please refer to: Eppendorf centrifuges
Can I store my cell culture products in the biosafety cabinet during UV-Sterilization?
Eppendorf Cell Culture products are not resistant to longer UV-radiation with an intensity that is usually used for UV-sterilization of biosafety cabinets.
What is the temperature range the Eppendorf Cell Culture Consumables can be used?
Eppendorf Cell Culture Consumables are stable in the range of -86°C to 60°C.
Do the Eppendorf Cell Culture Consumables promote the attachment and proliferation of adherent cells?
We offer tissue culture (TC) treated or non-treated cell culture consumables. The TC treated products promote adhesion of adherent cells on the surface. The TC treatment procedure generates charged groups on the usually hydrophobic polystyrene surface. This facilitates cell attachment and spreading of most anchorage dependent cells.
What can be the cause for poor cell attachment?
Multiple factors can influence cell attachment or growth. For instance serum-reduced or serum-free conditions or transfection of cells can result in poor cell attachment. Some cell types require a surface with biological coatings like fibronectin, poly-D/L-lysine, collagen etc.
What is the chemical resistance of Eppendorf Cell Culture Products?
Please contact your local Eppendorf Application Support for information on chemical resistance.
Are the Eppendorf Cell Culture Flasks certified leakage proofed?
Yes, a 100% in-line pressure test for each flask guarantees leakage-free flasks for maximum workflow safety.
What filter technology does Eppendorf use for the filter cap and what benefits does this technology offer?
The Eppendorf filter cap is equipped with a high efficiency hydrophobic air filter. This new filter technology combines improved protection with reliable gas exchange. Due to its hydrophobicity the filter repels liquid splashes effectively.
How do I use the black bend-seal on the dish package?
All Eppendorf Cell Culture Consumable multipack packages are resealable to provide maximum product safety. The packaging of the dishes has – in addition to the sticky sides for resealing - a bend seal for extra safety and space saving storage.
What is the purpose of the ring in the lid of the dish?
The SplashProtect™ ring inside the dish lid traps liquid drops which may occur from spillages during transportation. This reduces the risk of cross-contaminations and gives an extra security during all handling and transport of dishes.
Why is the 96-well plate equipped with an outer moat?
The outer moat of the 96-well plate provides the option to reduce the edge effect: It can be easily filled with liquid (5 mL) to reduce evaporation. Find more information about reducing the edge effect in Application Note No.326.
Can I use Eppendorf Cell Culture Plates for automated systems?
The Eppendorf Cell Culture Plates conform to the SBS standard. Therefore they fit to most automated systems.
Does the SplashProtect™ ring inside the dish lid impairs the gas exchange?
Gas exchange is facilitated by the vented lid design. The SplashProtect™ ring inside the lid has no influence on the gas exchange.
Where do I find the order number and the lot number?
You can find the order number as well as the lot number on the primary sleeve packaging as well as on the outer box packaging. In addition, you will find the lot number on each Eppendorf Cell Culture Flask.
Can any of the Eppendorf Cell Culture Consumables be reused?
We do not recommend
reusing the Eppendorf Cell Culture Consumables regardless of the format. All products are
intended for single use only.
Are your products certified sterile, free from Endotoxins and DNA, DNase and RNase?
Yes, all Eppendorf Cell Culture Consumables are certified non-cytotoxic (absence of
pyrogens) and free of human and bacterial DNA, DNase and RNase. Sterilization is performed
by irradiation according to DIN EN ISO 11137-2:2007 or ethylene oxide sterilization with a SAL
(sterility assurance level) of 10-6. For detailed information please refer to the respective
certificate of quality and the Lot-specific certificates.
What are the recommended working volumes for the different formats?
For information regarding working volume, maximum volume, and growth area of the different formats
please refer to the Eppendorf Cell Culture Consumables user manual chapter 4/technical data.
Are your products free of BSE/TSE?
Eppendorf only works with granulate suppliers who
guarantee that their used animal components come exclusively from countries without BSE
occurrences. Risky materials are not used. The granulate production includes process steps
with conditions above 235°C/ 3,000EkPa for several hours that destroy any virus, bacteria or
substance that cause immunological diseases (TSE, BSE, CJD). For detailed information
please refer to the respective certificate of quality and the Lot-specific certificates.
What is the temperature range the Eppendorf Cell Imaging Consumables can be used?
Eppendorf Cell Imaging Consumables are resistant over a wide range of temperatures (see table here
), temperature shifts will not distort or harm the material. Chamber and lid of Eppendorf Cell Imaging Slides and Coverglasses are made of a highly temperature resistant Polymer and are therefore also best suited for applications requiring high temperatures, e.g. in-situ hybridization.
Do the Eppendorf Cell Imaging Consumables promote attachment and proliferation of adherent cells?
All Eppendorf Cell Imaging Consumables are tissue-culture (TC) treated for improved adhesion of cells on the glass (Eppendorf Cell Imaging Dish, - Slide, - Coverglass, - Plate with glass bottom) or film bottom surface (Eppendorf Cell Imaging Plate with clear film bottom).
Is there a difference regarding surface treatment between Eppendorf Imaging Consumables (cover glass, glass, film bottom) and Eppendorf Cell Culture Consumables (polystyrene)?
Both - Eppendorf Cell Culture Consumables and Eppendorf Cell Imaging Consumables are tissue-culture (TC) treated. Although the methods applied are slightly different due to different material properties of polystyrene, film and glass all TC-treatments are chemical modifications (no coatings) generating functional groups covalently bound to the base material. This surface modification results in an improved adhesion of anchorage dependent cells.
Do I have to use biological coatings like Fibronectin, Poly-D/L-Lysine or Collagen to make the cells adhere to the surface?
The surface of all Eppendorf Cell Imaging Consumables is tissue-culture (TC) treated meaning that it promotes the attachment of most adherent cell types. However, some sensitive primary cells or cell lines might make it necessary to coat the surface prior to their cultivation.
What is the shelf life of Eppendorf Cell Imaging Consumables?
All Eppendorf Cell Imaging Consumables have a shelf life of 5 years after date of production.
How long do Eppendorf Cell Imaging products stay sterile in the closed packaging?
As long as you keep the packaging closed the products are sterile. Eppendorf guarantees a sterility of 5 years for unopened packages.
Do the adhesives that are used for the Eppendorf Cell Imaging Consumables have any influence on the cells?
The adhesives used for Eppendorf Cell Imaging Consumables are non-cytotoxic and biocompatible according to DIN EN ISO 10993-5:2009.
Which Eppendorf Cell Imaging Consumables are suitable for live cell imaging?
All Eppendorf Imaging Consumables with cover glass and film bottom are best suited for studying living cells: Cell Imaging Dishes, Cell Imaging Plates and Cell Imaging Coverglasses.
Which Eppendorf Imaging Consumable would you recommend for hypoxia-studies?
Eppendorf Imaging Plates with clear film bottom are best suited for cultivating cells under hypoxic conditions or oxygen-controlled conditions, respectively. The 25 µm fluorocarbon film bottom allows optimal gas transfer between cell culture medium and the surrounding Incubator atmosphere.
Can any of the Eppendorf Cell Imaging Consumables be reused?
We do not recommend reusing any of the Eppendorf Cell Imaging Consumables regardless of the format. All Eppendorf Cell Imaging Consumables are intended for single use only.
Which immersion oils are compatible with Eppendorf Cell Imaging products when using oil immersion objectives?
Eppendorf Cell Imaging Consumables are compatible with all standard immersion oils. The use of immersion oil does not lead to damage of the glass or film bottom material.
Is the plastic surface of the dish also tissue-culture (TC)-treated?
Yes, the glass area as well as the whole surface of the Cell Imaging Dish is TC-treated to support attachment of adherent cells.