Cell viability was evaluated after treatment with a staurosporine dose-response curve. Profiles generated were comparable for each instrument used for cell seeding and reagent adding. Nevertheless, with a manual pipetting system, the assay reproducibility was affected as indicated by higher standard deviations.
Cell-based assay performance was analyzed in a number of different ways. The signal-to-background ratio (S/B) is often used as distinction of assay performance, even if it does not take assay variability into consideration. For this reason, the Z’-factor, a statistical value, is the industrial standard used as an indicator of assay robustness.
With all assay performance parameters studied (signal-to-background ratio, Z’-factor, intra-plate and inter-plate CV), it was demonstrated that the assay robustness and reproducibility were significantly better when an electronic pipette (epMotion 96 or electronic pipette) was used.
Among all instruments tested, the epMotion 96 appears as the fastest option since a complete cell-based assay requires only 4 minutes, that means 5 times less hands-on time than what is needed with the manual pipette.
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