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Centrifuge Solution for Plasmid construction, Sequencing preparation and Humanization
Target antigen production, sequencing sample preparation, and antibody humanization involve a great deal of repetitive molecular biology handling steps. This includes spin column based MiniPrep kits for nucleic acid purification, gel extraction, and PCR clean-up. Each protocol has multiple centrifugation steps interrupted by pipetting and vessel changes. Microcentrifuges have entered a new generation, with the completely newly developed Centrifuge 5425 in combination with kit rotor specifically designed for 1.5/2.0 mL spin column kits.
Key benefits
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INCREASED EFFICIENCY
- Rotary knobs control for faster changes of speed and time.
- Fast access to your most frequently used settings with three custom program buttons.
- One push short-spin key for quick centrifugation – no need to hold key all the while.
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IMPROVED SAMPLE SAFETY
- Reduced risk of cross-contamination in spin column applications due to extended rim that serves as reinforcement for open tube caps during centrifugation and protects caps from shearing off.
- Automatic lid opening at the end of the run prevents sample warming and allows easy access to samples.
- Integration of incubation steps via timer function for increased reproducibility.
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BETTER ERGONOMICS
- Short runs up to 12 minutes are whisper quiet at an average of 45 dB(A). This is sufficient for centrifugation steps of most routinely used DNA/RNA purification kits. During longer runs the cooling fan works at full capacity.
- QuickLock rotors close with only 1/4 of a turn. This not only saves you time during every day use, it also takes repetitive stress off your wrist.
- Soft-touch one-finger lid closure for ergonomic lid locking.
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Components
Centrifuge 5425
- Whisper quiet 24-place standard microcentrifuge with outstanding feature set for broad range of applications, non-refrigerated.
- Extended rotor program (e.g. 5.0 mL rotor, swing-bucket rotor for PCR strips).
- Max. capacity: 10 × 5.0 mL vessels.
Rotor FA-18x2-Kit
- Fixed-angle rotor for molecular biology applications in 18 × 1.5/2.0 mL tubes or spin columns.
- Aerosol-tight with Eppendorf QuickLock lid.
- Max. speed: 18,565 × g (15,060 rpm).
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Recommended Consumables
Key Methods | Consumables | Advantages |
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Learn about all Centrifuge Solutions for mAb
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