If I assign my softkeys in the application mask My Application with the 2 functions, e.g. Z off and Y off, I can’t activate both softkeys at the same time. What should I do?
It would be possible to assign in addition a softkey with X only and to activate this function if both Z and Y axes should be deactivated at the same time.
I saved a position, however, when I recall it, the needle does not move as far down as it it was programmed.
You have probably set in the meantime a limit in the vertical plane (Z-axis). Delete the limit and then activate the stored position again.
I would like to change my speed from fine to x-fine, but the function key does not react.
When the speed/radius of Xfine is set to 0 in the menu, changing to Xfine cannot be done with the Fine/Xfine key. Set the speed/radius in the Menu/Speed --> x-fine greater 0.
I would like to use the Step Injection function for TransferMan 4r, but I do not have an electronic Eppendorf injector. How can I trigger the injection movement?
You can use our foot control for this, it can be ordered as a non-standard accessory, the article number is 5181 150.051. Please note, when using an injector which does not belong to the Eppendorf FemtoJet family it is not possible to trigger automatically both injection pressure together with the injection movement. You have to trigger the injection pressure seperately at your injector.
I always use fine mode for cell/sperm collection and subsequent cell injection, but would like to gather the next lot of cells/sperms quickly. The dynamic range of the DualSpeed joystick of the TransferMan 4m/r is much too slow for me. Do I therefore have to switch between fine and coarse constantly?
That would be an option. However, you can also increase in the menu the speed of the outer dynamic range (Dyn-mode) of the joystick. It is then still possible to inject slowly and carefully using the fine speed mode for your joystick, and to gather more cells/sperms, etc. at an x% higher speed without switching to the coarse mode. To do this, set in Menu/Installation/Dyn-factor the Dyn-mode to 1000% max.
When I move the joystick the motors do not move as expected, what should I do?
It is possible that the plug connections for the motors were set incorrectly on the control panel. Please check it against the identification on the cable sleeve and the description of the plug connection on the control panel.
Can I set up the TransferMan 4m/r or InjectMan 4 together with one of the predecessors NI2/ NK2/ NP2 on one microscope workstation?
Yes, it is possible if a microscope adapter for the previous generation has been already installed. You need only to set an adapter bridge (order information 5192 321.001) on the side where the new manipulator is to be installed.
I have a very large/low microscope condenser set-up at my manipulation workstation. How can I prevent my capillary holder or motors hitting the condenser when I trigger the Home function?
This is fairly simple - you can set an ‘Upper limit‘, i.e. a limit in the Z axis, via the Installation menu function. When the capillary then moves out of the working range to the Home function, the Z motor will stop at the defined upper limit.
If I want to move my capillaries upwards out of view, the motor stops although I can clearly see on the scale marking of the Z-motor that it could move up further.
It is possible that an Upper limit has been defined for the Z axis in the installation menu. Please check this on the display: If you move the motor as far as it will go and an upper limit has been defined, a 'U' will appear next to the µm display of the Z axis, e.g. Z: 1237 µm U. You can delete or change the limit at any time by going to Upper limit in the installation submenu.
Can the TwinTip holder be attached to the manipulators TransferMan 4m/r or InjectMan 4?
Unfortunately, this is not possible. However, we will be happy to provide a PiezoXpert for demonstration. Perhaps you could test our Piezo-assisted manipulation system instead of Tyrode’s Acid and see if this alternative is suitable for your application.
When the Home function is activated/triggered there is a softkey function shown in the display called "Back manual". What is it for?
Back manual is a function key that allows you to interrupt the activated Home function which moves your capillary automatically out of the work position into the Home position for capillary exchange and automatically back: when pressing the Back manual key you can move your capillary from the Home position manually back to your work position via the joystick instead of pressing the home key again.
When the Home function is activated/triggered there is a parameter shown in the display called "Offset", what is it for?
The function Offset can be used when the capillary or the Petri dish has been changed with help of the Home function but you are not sure if the original work position of the capillary might be too low (risk of capillary breakage) for the new petri dish/capillary. A programmed Offset of 500 µm for instance will bring the capillary from the Home position back to the original work position but 500 µm above. The capillary will still be in optical view but safely above the bottom of the petri dish. The Offset parameter can be set using the up- and downwards arrow softkeys displayed under the Offset parameter.
Can a PC be used to control the TransferMan 4r or InjectMan 4?
A PC can be used to control the Eppendorf manipulators. Commands in ASCII format can be found in the supplement of the manual, referred to as Cell Technology PC control. Windows HyperTerminal or TuTTY are required, therefore an RS232 connection cable has to be used (order number 5181 150.094).
Can the TransferMan 4r also be used for injecting into adherent cells?
In principle it is possible to use the TransferMan 4r for injection into adherent cells as well. If this manipulator is connected with the Eppendorf microinjector FemtoJet, you can use the Step injection function in combination with axial movement to inject into adherent cells. The injection movement will then be triggered via hand or foot control of the FemtoJet. However, our InjectMan 4 is much better suited for this application. The unique semi-automated injection function with its automated Z movement, triggered by the joystick of the InjectMan 4, makes microinjection into adherent cells very quick and easy.
What should the working distance be for an upright microscope?
The minimum working distance is approx. 5 mm. In this case, the capillary must be mounted at a very flat angle, almost parallel to the table. In general, inverted microscopes are a better choice for micromanipulation experiments because of their large working distance of more than 20 mm.
How many adapters do you need to mount two manipulators on one microscope?
Only one adapter is required.
Can the injection angle be varied to suit different applications (e.g. axial injection of DNA into the gonads of worms)?
Yes. The angle can be adjusted for different applications. The adjustable angle head allows angles from 0°-90°. The angle settings for axial injections must be also changed in the menu of the manipulator (go to: Menu/Installation/Angle). No additional adapters are necessary.
What are the scale markings on the motor modules for?
The scales are for easy adujustment of the modules when installing. A guide will be prepared with information on the installation of modules on different microscopes.
What is the "Dyn-factor" in the menu (Menu/Installation)?
This parameter allows to set or to increase the speed of the dynamic movement range (outer ring of the DualSpeed joystick) in relation to the direct (=proportional) movement control modus of the joystick.
What are the major improvements of the new manipulator TransferMan 4m/r compared to the predecessor TransferMan NK 2?
Major improvements are: i) Unique DualSpeed Joystick: proportional (direct) movement control of the capillary PLUS dynamic speed modus when touching with the joystick the outer ring of the joystick base; ii) improved precision and stability, exceptional dynamic and static stability; iii) ultra shallow angle of capillary holder (<5°) (no need of additional 10° adapter); iv) new operation concept with preprogrammed application-specific masks.
After changing the softkeys of each application option, can they be reset to default setting?
Yes. Select Function in the menu. Execute the User Default option to activate the default setting.
Is it possible to switch the DualSpeed joystick of the TransferMan 4m/r to complete dynamic movement range?
No. Only the outer ring of the joystick base is designed for the dynamic movement option of the motors. All joystick movements that are operated within the inner range of the joystick base are translated in direct and precise 1:1 motor/capillary movements.
How can I benefit from the Step injection function?
The Step injectionfunction can be used when TransferMan 4r or InjectMan 4 is connected to the electronic microinjectors of the Eppendorf FemtoJet family: With this electronic connection a defined forward microinjection movement togehter with a defined injection pressure can be carried out automatically. You can adjust the speed (µm/s) and the travel distance of the injection movement (µm), the direction of the injection (straight forward or axial) and the time when the injection pressure should be given. Any other injection pressure parameters are to be set at the connected injector.
Can the application names on the display be changed?
No. It is not possible to change the application names. However one can customize his/her own softkeys using the „My Application“ option.
What are the differences between TransferMan 4m/r and InjectMan 4?
The TransferMan 4m/r is a device designed for direct, intuitive handling of sensitive samples, as required in ART or similar techniques. The InjectMan 4 focuses on applications where speed, throughput and dynamic forces are required. Examples would be injection into adherent cells or serial injection in larger specimen like fish embryos or C. elegans. Though the motor modules are the same, there are a number of differences between both manipulators. The most important difference is the control of the motor movement. Whereas the InjectMan 4 motors are controlled via a dynamic joystick, thus enabling fast, dynamic capillary movements, the TransferMan 4m/r operates with a unique DualSpeed joystick. The DualSpeed joystick allows direct, intuitive movements of the motors/capillaries for handling of sensitive objects plus a fast, dynamic movements when using the outer dynamic zone of this special joystick. The InjectMan 4 features the well-known semi-automated injection function. Electronic coupling with the Eppendorf FemtoJet injectors allows performing the unique axial-injection movement plus the injection pressure given by the FemtoJet just by triggering once the joystick key of the InjectMan 4. Furthermore the two manipulators display different preprogrammed application masks to choose from: Since both principles of movement target different applications, the application masks are adjusted accordingly.
Does the micromanipulator remains the last setting even after switching off?
Yes, stored parameters (like positions, limits as well as the deactivation of single motor modules) do not get lost after switch off, in case the application mask has been stored before as the default application mask to start with (either by pressing the respective softkey longer than 3 secs on the Start display or by selecting the application using the menu) and the Standby function is used before using the mains power switch at the back of the control board.
Do the coordinate values on the display of the joystick control unit refer to the position of the capillary?
No. It refers to the position of the motor modules.
Can the angle head be used for both, right and left side mounting of the motors?
Yes. It can be used on both sides. For optimal mounting the blue angle screw has to be positioned at the top-side of the angle head, please refer to the instruction manual.
Can other non-Eppendorf devices be electronically connected to TransferMan 4r or InjectMan 4?
No. Only the electronic injectors of the FemtoJet family and the PiezoXpert can be connected to the manipulators.
What do I need to consider when I connect the Eppendorf PiezoXpert to the TransferMan 4r or InjectMan 4?
For the connection a special cable is required (order number 5181 150.094). Eppendorf PiezoXpert devices with a software version below v.1.2.1 will require a software update.