PCR Basic Training
Laboratory personnels, principal investigators, technicians, researchers or postgraduate students who wish to understand PCR theory and improve their PCR skills and techniques. For individuals who are looking to perform molecular work and or currently performing molecular work/PCR.
Objective of seminar:
This course will cover basics of molecular biology, including DNA extraction and PCR setup. Participants will become familiar with the principles behind PCR mechanims, optimisation and develop practical skills to peform and troubleshoot PCR-based experiments in research.
Content of seminar:
> Basics of molecular biology
> DNA extraction
> PCR basics
> Setting up a PCR assay
> DNA Extraction and Quantification
> Routine PCR and Gel electrophoresis
> Gradient PCR - Annealing and Denaturation