Seminar Details

Basics of Molecular Biology: Introduction to DNA Cloning


Technical or scientific personnel with minimum knowledge of molecular biology. Participants must have basic experience of working in a laboratory.


This course will cover understanding of the structure and function of cells and nucleic acids, including mechanism of genetic information transfer from DNA to RNA and protein. Participants will become familiar with the principles behind molecular biology experiments and develop practical skills to implement these methods in research. Participants will also learn the principles of PCR and apply this knowledge to clone PCR products.


Theoretical part:

> Basic Molecular Biology
> PCR Cloning
> Transformation
> Plasmid Extraction
> RNA and preparation for RNA experiment
> Experimental design   

Practical part:

> Genomic DNA extraction & DNA quantification
> Gel electrophoresis
> PCR product purification
> Ligation
> Bacteria cell transformation
> Inoculation of blue & white colonies for plasmid extraction, Plating
> Calculation of transformation efficiency
> Plasmid extraction & quantification
> Restriction digestion    



350 USD税込み VAT

最小 6参加者数

最大 8参加者数

Eppendorf Training Center, Kuala Lumpur, Malaysia

Booking Information for Eppendorf Asia Regional Center courses

Important Information

The scheduled courses will run with a minimum of 4 persons. For groups with minimum 6 persons from a single organization you may also request a date of your choice (subject to availability).

Tea and lunch are included in the cost, however travel and hotel costs are to be borne by the participant. A participant who registers for two courses or more is entitled to a 15% discount, likewise a single organisation who sends two or more participants is also entitled to the same discount.

Registration is only valid with payment to your local dealer or Eppendorf Organisation and a refund will be given if cancellation is made more than 2 weeks prior to a course. If less, then a 50% discount is given.

All courses are conducted in English. If you would like a customised course other than the ones offered above, please contact us using the contact information on the registration form.

Upon successful completion of the course, you will receive a certificate of attendance.

The training does not stop there with the courses we provide, you can expect continuous support from our in-house network of application specialists and technical engineers , who can always be contacted by phone or email.

Your contact person is:

  Hajar Fadin Binti Baharudin
  Tel.: +603 7610 5202