Basics of Molecular Biology: Introduction to DNA Cloning
Technical or scientific personnel with minimum knowledge of molecular biology. Participants must have basic experience of working in a laboratory.
This course will cover understanding of the structure and function of cells and nucleic acids, including mechanism of genetic information transfer from DNA to RNA and protein. Participants will become familiar with the principles behind molecular biology experiments and develop practical skills to implement these methods in research. Participants will also learn the principles of PCR and apply this knowledge to clone PCR products.
> Basic Molecular Biology
> PCR Cloning
> Plasmid Extraction
> RNA and preparation for RNA experiment
> Experimental design
> Genomic DNA extraction & DNA quantification
> Gel electrophoresis
> PCR product purification
> Bacteria cell transformation
> Inoculation of blue & white colonies for plasmid extraction, Plating
> Calculation of transformation efficiency
> Plasmid extraction & quantification
> Restriction digestion