Can contamination of micro test tubes by bacterial endotoxin be inactivated by autoclaving?
No. Bacterial endotoxins (lipopolysaccharide, LPS) are very stable and withstand normal autoclaving at 121 °C. To inactivate LPS, the objects have to be baked at 250 °C, which, of course, is not possible with plastics.
Are the Eppendorf micro test tubes anti-static?
Our tubes are not produced from antistatic material, i.e. they are basically not antistatic. But it should be noted that the use of antistatic agents during production will destroy or reduce the wettability of the tubes.
Which Eppendorf micro test tubes are free of DNA?
All micro test tubes designated with "PCR clean" are free of human DNA, DNase, RNase and PCR inhibitors. Our Safe-Lock micro test tubes are also available in the "Eppendorf Biopur" purity standard. Due to the special manufacturing processes and the individual blister-packaging, Biopur products are certified "DNA free".
What level of protein binding affinity do your micro test tubes demonstrate?
Our micro test tubes are used both in the proteomics area and for storage and transport of many different protein solutions. If you work with low protein concentrations, we recommend using the Eppendorf Protein LoBind Tubes. These tubes are made of a special type of polypropylene and are manufactured under optimized process conditions. In comparison to standard polypropylene tubes, these tubes therefore demonstrate a considerably lower affinity to proteins.
How do you define the temperature entry "RT", for example, as a storage condition for your micro test tubes?
Definition of RT (room temperature): +15 to +25 °C.
What is the best way to label Eppendorf micro test tubes?
The micro test tube should be clean and free from grease and only labeled on the space provided. Water-resistant felt pens from Steadtler S and F, in black, are the most suitable for this. The writing should be left to dry for at least 2 min.
Do your micro test tubes contain heavy metals?
Lead, cadmium, mercury, etc. are well below the prescribed limits of the DIN norm. In the course of the production process it is impossible to avoid the smallest quantities of metal resulting from machine friction.
Overview regarding the centrifugation resistance of Eppendorf tubes
Safe-Lock 0.5 ml: 30,000 x g / Biopur 22,000 x g / DNA LoBind 30,000 x g / Protein LoBind 18,000 x g
Safe-Lock 1.5 ml: 30,000 x g / Biopur 22,000 x g / DNA LoBind 30,000 x g / Protein LoBind 18,000 x g
Safe-Lock 2.0 ml: 25,000 x g / Biopur 22,000 x g / DNA LoBind 25,000 x g / Protein LoBind 18,000 x g
3810X: 30,000 x g
3810: 25,000 x g
When using form-fitting tubes and buckets and filling the tubes with their nominal volume, centrifugation of the Safe-Lock Tubes at 25 °C for 30 min at up to 70,000 x g (RT) is possible. Tubes can be centrifuged in a 45°-fixed-angle rotor at 40 °C sample temperature and 90 min with aqueous saline solution (density 1.0 g/ml) up to the above g-forces. The centrifugation resistance depends on the temperature and will be lower at sample temperatures of > 40 °C. Liquids with a higher density or affect the stability of the tubes (organic solvents, acids) may reduce the mechanical resilience of the tubes.
Are the Eppendorf tubes autoclavable?
Yes, all Eppendorf tubes are autoclavable when open (20 min, 121 °C, 1 bar).
Do the Eppendorf LoBind Tubes have the same material durability as all other micro test tubes from Eppendorf?
The Eppendorf LoBind Tubes completely satisfy the specifications of the Eppendorf Standard Safe Lock tubes with reference to centrifugation stability (exception: Protein LoBind Tube), lid tightness, pop up safety and chemical resistance. (See Application No. 56: The best material for original Eppis.)
Are the Eppendorf LoBind Tubes coated?
Not the Eppendorf LoBind Tubes are neither siliconized nor coated. The low binding affinity results from of a special type of polypropylene and the optimized manucturing process.
Can I use the Protein LoBind Tubes regardless of whether I am working with basic or acidic proteins?
The Protein LoBind tubes show protein low binding properties for both low and high pH values.
What distinguishes the Protein LoBind Tube from a "Standard" Safe-Lock Eppendorf tube?
The Protein LoBind Tube shows, in comparison to a Safe-Lock Tube, significantly less protein binding affinity. The Protein LoBind Tube is therefore especially suitable for applications with the lowest quantities of protein.
Are 1.5 ml Safe-Lock micro test tubes safe for use in the food industry in USA?
The composition of the 1.5 ml Safe-Lock micro test tubes conforms to the specifications of the FDA regulation 21 CFR § 177,1520 "Olefin polymers". Please note: The suitability of a consumer item for the intended purpose, the influence of the taste and smell of the filled goods and the conformation to limits (e.g. global migration, specific limits and further analytical requirements) must be checked with the manufacturer or user in individual cases.
Are 1.5 ml Safe-Lock micro test tubes safe for use in the food industry in Europe?
Our 1.5 ml Safe-Lock micro test tubes are suitable for use in the food industry according to the Food and Consumer Goods Legislation § 5, paragraph 1 no. 1 (food and consumer goods). Please note: The suitability of a consumer item for the intended purpose, the influence of the taste and smell of the filled goods and the conformation to limits (e.g. global migration, specific limits and further analytical requirements) must be checked with the manufacturer or user in individual cases.
Do your Safe-Lock micro test tubes contain polyethylene glycol (PEG)?
No. All our test tubes are made from pure polypropylene. The material does not contain PEG.
In which temperature range can the Safe-Lock micro test tubes be used?
They can be used from -80 °C to +100 °C. However at temperatures of > 95 °C the tube lids are more likely to pop open. For purposes of autoclaving, the tubes can be heated up to 121 °C.
To what extent are the amber colored Safe-Lock micro test tubes permeable to fluorescent dyes?
The amber colored Safe-Lock micro test tubes protect from light in the spectrum from 700 nm to the UV spectrum of 190 nm. In the spectrum of visible light (350 - 600 nm) the protection is optimal with lessening intensity. At approx. 550 nm (excitation wavelength for Cy3) the measured deletion is app. 1, meaning that 90 % of the incident light is blocked by the walls of the tube. At 650 nm (excitation wavelength for Cy5) the measured deletion is app. 0.35, meaning that app. 65 % of the incident light is blocked by the walls of the tube. If that is not sufficient, we recommend wrapping the tube in aluminum foil.
How are the sterile Eppendorf Plate Deepwell packed?
The plates are packed stacked in a resealable ZipLock bag.
Are Eppendorf Plate® Deepwell together with the colored border resistant against organic solvents?
Eppendorf Plate® Deepwell are of polypropylene. All parts of the plate (colorless/colored parts and coding) have the same high resistance against chemicals and are resistant against many organic solvents. For details please see our Application Note no. 56: The best material for original Eppis. In case of doubt please contact Eppendorf Application Support: firstname.lastname@example.org.
Is it possible to store Eppendorf Plate® Deepwell at –196°C in liquid nitrogen?
No - we recommend storage at –80°C.
Are Eppendorf Plate® Deepwell autoclavable?
Yes, but the dimensional precision of the plates can be affected. If the use of sterile plates is needed we recommend Eppendorf Plate® Deepwell Sterile.
Are Eppendorf Plate® Deepwell LoBind coated or siliconized?
No - the plates are neither coated nor siliconized.
Does the polypropylene of the plates contain plasticizers?
No – Eppendorf Plate® Deepwell are made of polypropylene which does not contain plasticizers.
Eppendorf Plate® Deepwell are withstand high centrifugation forces – what are the limits?
Eppendorf Plate® Deepwell Standard and LoBind Quality can be centrifuged up to 6000 x g. Eppendorf Plate® Deepwell Sterile withstand up to 5000 x g. For details refer to the instruction manual.
What are the precise dimensions of Eppendorf Plate® Deepwell?
Please see for that Plate Dimensions included in the Technical Information on our homepage
Are Eppendorf Plate® Deepwell available with Barcode?
Yes - Eppendorf Plate®
Deepwell are optional available with Barcode. Detailed ordering information can be found under our homepage
Which advantages do Eppendorf Polypropylene Microplates have over plates made from polystyrene?
Plates made from polypropylene may be used across a broad spectrum of applications, as they are more resistant to temperature variations as well as various chemicals than plates made from polystyrene. Furthermore, they are more robust, allowing them to be centrifuged at higher g-forces. In addition, biomolecules, such as nucleic acids and proteins, bind less strongly to polypropylene.
Are Eppendorf PP Microplates resistant to organic solvents, e.g. DMSO?
The Eppendorf PP Microplates are made from polypropylene and are thus resistant to DMSO and other organic solvents, unlike plates made from polystyrene. An overview of chemical resistance can be found in Application Note no. 56. In case of doubt please contact Eppendorf Application Support: email@example.com.
Are the colored rim and labeling resistant to organic solvents?
All areas of the plate, including the colored rim, are resistant to chemicals. The alpha numeric labeling is equally durable. An overview of chemical resistance can be found in Application Note no. 56. In case of doubt please contact Eppendorf Application Support: firstname.lastname@example.org.