Microbiology

Microorganism

Microbiology is the study of microorganisms, a large and diverse group of organisms that exist as single cells or cell clusters. It is a broad biological discipline which includes basic research as well as applied science. The identification of microorganisms is part of medical microbiology as well as quality testing e.g. in pharmaceutical and food industry. Examples for the usage of microbes in industrial processes include fermentation (e.g. production of food & beverages, drugs, vaccines, biofuels, enzymes) and waste-water treatment.

  1. Transformation
  2. Cultivation
  3. Preparation & Purification
  4. Detection & Amplification
  5. Storage

Transformation

Electroporation can be used for the insertion of DNA into electro competent bacteria and yeasts. Compared to chemical methods, electroporation yields much higher transformation efficiency. It also gives more reproducible results than other methods.

  • Electroporator
    • Faster sample handling - simple one-button operation
    • Maximum safety via integrated cuvette holder
    • Self-explanatory operation guided by display indicators
    • Fast, freely programmable softkeys for saving and accessing
      the 2 most frequently used parameters
    • Compact, space-saving design
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  • Centrifuges
    • Max. rotor capacity: 24 x 1.5/2.0 mL vessels
    • Max. speed: 20,238 × g (14,680 rpm)
    • OptiBowl® design for quiet operation, even without a rotor lid
    • Soft-touch one-finger lid closure for ergonomic operation
    • SOFT brake to protect sensitive samples
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  • Tubes
    • Hinged Eppendorf Safe-Lock lid prevents unintentional lid opening during incubation and storage
    • Polypropylene provides high resistance to chemicals, mechanical stress and temperature extremes
    • Manufactured without the use of slip agents, plasticizers, and biocides – substances that have been shown to leach from plastic consumables into the sample and negatively affect bio-assay results
    • g-Safe®: Exceptional centrifugation stability up to 30,000 x g prevents sample loss due to tube breakage and provides extra safety when working with hazardous samples
    • Precise lid sealing for minimal evaporation rates during long-term storage
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  • Thermo mixing devices
    • Heating, mixing and cooling in all common vessel and plate formats from 5 µL to 50 mL
    • Increased mixing frequency up to 3,000 rpm (depending on the SmartBlock used)
    • Excellent mixing performance due to unique 2DMix-Control technology
    • Anti-spill technology prevents lid wetting and cross-contamination
    • Reliable prevention of condensate formation with the new ThermoTop (condens.protect®)
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Cultivation

The New Brunswick Shakers are robust and flexible shakers that provide very broad temperature capabilities for culturing a wide variety of organisms. With our comprehensive range of bioprocess solutions microorganisms can be cultivated under controlled conditions, e.g. using single-use vessels. 

  • Shakers
    • Stackable – up to three units for maximum space savings
    • Programmable Innova controls automatically changes temperature, speed and optional photosynthetic and UV germicidal lights at timed intervals
    • Versatile accessory platform (sold separately) accommodates flasks
      up to 5 L
    • Slide-out platform mechanism — provides easy and effortless access
      to flasks located in the front and back of the shaker/incubator
    • Built-in water reservoir humidifies chamber to reduce sample evaporation, while also protecting unit from spills, includes a drain for easy cleaning.
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  • Bioreactors
    • Rigid wall stirred-tank single-use vessels covering working volumes
      from 65mL - 40L
    • Wide range of industry standard autoclavable glass bioreactors
    • Stainless steel sterilize-in-place vessels
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  • Pipettes & Tips
    • Repetitive dispensing modes decrease the number of movements by allowing the user to fill the tip once and dispense up to 100 times
      without a refill
    • Automatic Combitip recognition eliminates timeconsuming volume calculations
    • Wide dispensing range: 1 µL to 10 mL
    • Stress-free work via integrated step counter: dispensing procedures can be continued error-free after an interruption or distraction
    • Central Combitip ejector: fully emptied Combitip can be easiliy ejected with one hand
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  • Deepwell Plates
    • OptiTrack® matrix: 30 % faster sample identification and fewer pipetting errors via high-contrast alphanumeric labeling
    • Conical RecoverMax® well design: Optimized well geometry for maximum sample recovery and excellent mixing properties
    • Minimal remaining volume and high uniformity from well to well
    • Raised well rims and smooth surface for reliable closing, even with heat sealing
    • Easy and reliable stacking
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  • Photometers
    • UV/Vis spectral range 200 nm to 830 nm
    • Freely selectable wavelengths across the entire UV/Vis spectral range (increment: 1 nm)
    • Compatible with microliter measuring cells and standard cuvettes
    • Temperature controlled cuvette shaft in the BioSpectrometer kinetic (20°C to 42°C in 0.1 °C increments)
    • Fluorescence intensity measurements across a range of 0.5 nM to 2,000 nM fluorescein with the BioSpectrometer fluorescence
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  • Bioprocess Systems
    • Comprehensive and scalable hardware and software solutions for R&D, process development, pilot, and production
    • Small scale and scale down models, parallel operated bioreactors, bench scale solutions, and sterilize-in-place systems
    • Covering working volumes of 60mL - 2,400L
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Preparation & Purification

To prepare and purify your samples you can choose between automated or manual systems. The Thermomixers guarantee optimal mixing performance and temperature control for your set-up, and a broad range of consumables, pipettes and tips complete these options.

  • Centrifuges
    • Max. speed: 3,000 x g (4,400 rpm)
    • Very compact footprint to fit on every lab bench
    • Very quiet operation to benefit your environment
    • Low access height for simple loading and unloading of samples
    • SOFT brake function for slow acceleration and braking.
      Optimized for cell separation by gradient centrifugation
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  • Pipettes & Tips
    • The ultralight Research was developed according to the strict criteria
      of the Eppendorf PhysioCare Concept.
    • Eppendorf PerfectPiston®: Ultralight and highly resistant piston system
    • The positions and shapes of the control buttons/displays have been optimized for stress-free work procedures and intuitive operation
    • The spring-loaded tip cone provides you with an optimal pipette fit
      with minimal insertion force (not available for 5 mL and 10 mL pipettes)
    • The adjustment option allows you to perfectly adapt the pipette for
      specific liquids or other external conditions.
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  • Conical Tubes
    • Premium USP class 6 raw material and no use of slip agents, plasticizers, and biocides during manufacturing ensure highest sample integrity, high...
    • Cap with flattened and grooved sides supports a user-friendly ergonomic handling, enables a stable upright positioning, and minimizes contamination risk.
    • Reliable and safe labelling due to a large writing area on the tube and due to a flat and light-colored cap.
    • Precise dimensions enable maximum compatibility with centrifuge rotors, mixers and shakers.
    • Sterile, pyrogen-, DNase-, RNase-, and DNA-free. Each individual lot is checked and certified for quality and purity
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  • Thermo mixing devices
    • Heating, mixing and cooling in all common vessel and plate formats from 5 µL to 50 mL
    • Increased mixing frequency up to 3,000 rpm (depending on the SmartBlock used)
    • Excellent mixing performance due to unique 2DMix-Control technology
    • Anti-spill technology prevents lid wetting and cross-contamination
    • Reliable prevention of condensate formation with the new ThermoTop (condens.protect®)
    more... less...
  • Automation
    • 6 ANSI/SBS microplate positions
    • Compact control tablet with touchscreen or mouse operation, USB data transfer
    • Software assistants for simple, step-by-step application programming
    • Optical sensor1 for detecting liquids, labware, tips
    • Automatic replacement of 2 pipetting tools
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Detection & Amplification

With the Eppendorf Detection equipment a broad range of applications can be performed. Complementary cuvettes and plates are available. To run a PCR, our Mastercyclers and PCR consumables enable you to choose the perfect combination for your needs.

  • Photometers
    • UV/Vis spectral range 200 nm to 830 nm
    • Freely selectable wavelengths across the entire UV/Vis spectral range (increment: 1 nm)
    • Compatible with microliter measuring cells and standard cuvettes
    • Temperature controlled cuvette shaft in the BioSpectrometer kinetc (20 °C to 42 °C in 0.1 °C increments)
    • Fluorescence intensity measuruements across a range of 0.5 nM to 2,000 nM fluorescein with the BioSpectrometer fluorescence
    more... less...
  • Cuvettes
    • Suitable for measuring small volumes (≥50 µL)
    • UV and Vis-transparent between 220 nm and 1,600 nm
    • Two built-in optical path lengths in a single cuvette-just turn
      the UVette 90° to change from 10 mm and 2 mm
    • Volume markings for 500 µL and 1,000 µL
    • Available in individually packaged PCR clean and protein-free quality
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  • Thermocycler
    • Intuitive graphic programming
    • Small footprint
    • Universal block for ultimate flexibility
    • Connect up to 2 Mastercycler nexus eco to your existing unit
    • Available with gradient
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  • PCR Consumables
    • One-piece design: combining a polycarbonate frame and polypropylene wells for optimum performance
    • Extremely thin-walled polypropylene wells guarantee optimum heat transfer to the sample
    • Exceptionally solid and torque-resistant polycarbonate frame
    • Improved well-to-well tolerance
    • Cut-away corner and alphanumeric grid-referencing
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Storage

The New Brunswick freezers meet the highest standards for quality in regard to performance, sample security, and convenience. To concentrate your samples prior to the storage, the Concentrator plus is available as well as different Deepwell plates including sealing options.

  • Freezer
    • Provides 30% more storage capacity than freezers of equal size
    • Inner doors in upright models are gasketed and insulated creating three separate compartments minimizing cold air loss
    • Unique heated air vent with plunger to clear ice buildup and to relieve vacuum formation to gain immediate access to the freezer
    • Built-in voltage stabilizer on U535, U725, C585 and C760 freezers provides extra sample security
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  • Deepwell Plates
    • OptiTrack® matrix: 30 % faster sample identification and
      fewer pipetting errors via high-contrast alphanumeric labeling
    • Conical RecoverMax® well design: Optimized well geometry for maximum sample recovery and excellent mixing properties
    • Minimal remaining volume and high uniformity from well to well
    • Raised well rims and smooth surface for reliable closing, even with heat sealing
    • Easy and reliable stacking
    more... less...
  • Conical Tubes
    • Premium USP class 6 raw material and no use of slip agents, plasticizers, and biocides during manufacturing ensure highest sample integrity, high...
    • Cap with flattened and grooved sides supports a user-friendly ergonomic handling, enables a stable upright positioning, and minimizes contamination risk.
    • Reliable and safe labelling due to a large writing area on the tube and due to a flat and light-colored cap.
    • Precise dimensions enable maximum compatibility with centrifuge rotors, mixers and shakers.
    • Sterile, pyrogen-, DNase-, RNase-, and DNA-free. Each individual lot is checked and certified for quality and purity
    more... less...
  • Sealing options
    • Storage Film and Foil glued seals for simple and dependable sealing during sample storage
    • Heat Sealing Film and foil for continuous locking with the best evaporation protection (see heat sealing materials)
    • Foil products are made of aluminum, can be pierced, and protect light-sensitive samples
    • Film products are transparent and provide protection against unwanted punctures
    • Eppendorf Plate Lid: stable, flexible protection of samples during short-term storage
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  • Concentrator
    • Comes in 3 system configurations
    • Extremely quite operation <50 dB(A)
    • Chemical-resistant, maintenance-free PTFE diaphragm pump eliminates the need for changing up pump oil
    • Small footprint saves valuable bench space
    • Wide variety of 15 different rotor options to accommodate tubes
      from 0.2 mL to 50 mL, microplates and PCR plates
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