Stem Cells

Research & Scale-Up

Stem cell research is a broad field, dealing with embryonic and adult stem cells (e.g. mesenchymal and neural stem cells) or induced pluripotent cells. Scientists hope to gain valuable insights from the study of stem cells, e.g. in developmental biology. Understanding cell cycle control, cell specialization, and cell-host interactions are some of the challenges. In drug discovery, stem cells are evolving into cell culture models and tools for cell-based assays. Last but not least, cell therapy is on everyone’s lips with stem cells as the product itself to potentially cure a broad range of diseases. For commercialization, companies are looking into establishing the necessary infrastructures. They explore novel cell culture methods and expand cell lines in order to make cultivation processes reliable and scalable.

  1. Cultivation & Storage
  2. Genetic Engineering
  3. Characterization & Analysis
  4. Process Development
  5. Commercialization

Cultivation & Storage

Our cell culture consumables with tissue culture (TC)-treated surface allow optimal attachment and homogeneous growth of stem cells. The New Brunswick CO2 incubators provide the ideal environment for growing stem cells in culture. The speed adjustment of our Easypet helps to handle cells very carefully and precisely.

  • Dishes, Flasks & Plates
    • Unsurpassed safety in handling during transportation and while working due to corrugated handling ring
    • SplashProtect ring inside of the dish lid traps liquid and prevents spills during transportation or incubation
    • Robust stacking performance by pronounced rims on dish lid and exact fitting of lid and bottom when used in stacks
    • Easy differentiation of lid and dish bottom prevents unintended removal of the lid
    • Packaging combines tool-free opening, resealable top and side for tape-free closing and an easy solution to shrink the package for
      space-conscious storage
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  • CO2 Incubators
    • Standard features: Sealed inner glass door, reliable IR CO2 sensor, RS-232 communication port, 25 mm access ports
    • Three O2 control options (0.1 – 19 %, 1 – 19 %, 1 – 95 %) for hyperoxic or hypoxic studies, also available with split inner doors
    • Further Options: High Temperature Disinfection (4 hour at 120 °C), Copper chamber and interior for optimized protection against contamination, humidity alert package
    • Fanless design offers more usable space and eliminates the fan
      as a classic, and often repeated source of contamination
    • Six-sided direct heating system provides a uniform incubated environment to gently bathe cells
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  • Pipette controllers
    • Intuitive and convenient speed adjustment made simple with the tips
      of your fingers
    • Lightweight, well-balanced and ergonomic design that allows
      for fatigue-free pipetting
    • Vibrant backlit LEDs provide optical feedback of the remaining
      battery life
    • Lithium polymer rechargeable battery offers long cordless runtime
    • Smooth setting of pump speed
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  • Serological Pipettes
    • Individually wrapped
    • Easy to open paper/plastic wrap
    • Subunits with dispenser option to keep stored products safe
    • Harmonized and well-fitting operation with Easypet 3, compatibility
      with all existing pipet helper
    • Sharp and clear graduation for facilitated read-out
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  • Freezer
    • Provides 30% more storage capacity than freezers of equal size
    • Inner doors in upright models are gasketed and insulated creating three separate compartments minimizing cold air loss
    • Unique heated air vent with plunger to clear ice buildup and to relieve vacuum formation to gain immediate access to the freezer
    • Built-in voltage stabilizer on U535, U725, C585 and C760 freezers provides extra sample security
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Genetic Engineering

For transfection of cells, e.g. for reprogramming, Eppendorf offers a broad range of manipulation devices. A variety of general lab equipment is available to facilitate the set-up, starting with manual or automated pipetting devices and centrifuges that fit your needs.

  • Centrifuges
    • Max. rotor capacity: 48 x 1.5/2.0 mL, 6 x 50 mL, 2 x MTP
    • Max. speed: 30,130 x g (17,500 rpm)
    • Remarkable versatility with 12 different rotors
    • Soft-touch one-finger lid closure for ergonomic operation
    • Menu-driven, multi lingual operation menu (English, German,
      French, Spanish) with large backlit display
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  • Automation
    • 4 ANSI/SBS worktable and 3 virtual positions
    • Maximum pipetting accuracy from 1 to 1,000 μL
    • Calibrated 1-channel and 8-channel pipetting tools
    • Optical sensor1 for detecting liquids, labware, tips
    • Compatible with tubes (0.2 mL to 50 mL) and microplates with
      up to 384 wells
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  • Micromanipulators
    • Maximum stability ensures vibration-free work
    • One-hand operation for capillary and angle settings
    • Programmable Z-axis limit for preventing capillary breakage
    • Connection with Eppendorf PiezoXpert® and electronic microinjectors
    • Can be adapted to all conventional microscopes
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  • Tubes and Plates
    • Hinged Eppendorf Safe-Lock lid prevents unintentional lid opening during incubation and storage
    • Polypropylene provides high resistance to chemicals, mechanical stress and temperature extremes
    • Manufactured without the use of slip agents, plasticizers, and biocides – substances that have been shown to leach from plastic consumables into the sample and negatively affect bio-assay results
    • g-Safe®: Exceptional centrifugation stability up to 30,000 x g prevents sample loss due to tube breakage and provides extra safety when working with hazardous samples
    • Precise lid sealing for minimal evaporation rates during long-term storage
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  • Pipettes and Tips
    • The unique one-button operation offers a fast and ergonomic handling with reduced operating effort and active aerosol reduction. To avoid
      an accidential tip ejection a clear haptic feedback is provided.
    • The spring loaded tip cone improves user to user reproducibility
      and improved ergonomics by lowering tip attachment force
    • The Reference 2 multi channel offers a high flexibility incl a spring-loaded tip cone which can be switched on/off optionally
    • The secondary adjustment supports the easy adjustment of your Reference 2 for the most accurate pipetting of different liquids
      or other external conditions without the need for a full calibration.
    • The quick lock enables an easy cleaning and maintenance
      of your pipette
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Characterization & Analysis

Our Mastercyclers guarantee convenient and extremely fast PCR runs. In addition the gradient function can be used to optimize the PCR condition very easily and establish reliable protocols. With the Detection equipment further analysis such as UV/Vis absorption or fluorescence detection can be conducted.

  • Dishes, Flasks & Plates
    • Unsurpassed safety in handling during transportation and while working due to corrugated handling ring
    • SplashProtect ring inside of the dish lid traps liquid and prevents spills during transportation or incubation
    • Robust stacking performance by pronounced rims on dish lid and exact fitting of lid and bottom when used in stacks
    • Easy differentiation of lid and dish bottom prevents unintended removal of the lid
    • Packaging combines tool-free opening, resealable top and side for tape-free closing and an easy solution to shrink the package for
      space-conscious storage
    more... less...
  • PCR Consumables
    • One-piece design: combining a polycarbonate frame and polypropylene wells for optimum performance
    • Extremely thin-walled polypropylene wells guarantee optimum heat transfer to the sample
    • Exceptionally solid and torque-resistant polycarbonate frame
    • Improved well-to-well tolerance
    • Cut-away corner and alphanumeric grid-referencing
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  • Reader Plates
    • UV-VIS microplates feature an ultrathin film bottom for excellent light transmission in the UV range
    • Black Microplates offer an excellent signal-to-noise ratio - for clear signals even with low-concentration samples
    • White Eppendorf Microplates are optimized for highest sensitivity
      in the detection of luminescence signals by maximizing reflection
    • Solid black and white assay plates are made of polypropylene resulting
      in high resistance to chemicals, mechanical stress and temperature extremes
    • All plates are optimized for minimal autofluorescence and autoluminescence
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  • Cuvettes
    • Microvolume measuring cell for measuring 1.5 - 10 µL sample volumes
    • Low self-absorption (≤0.05 A @ 260 nm)
    • Hydrophobic surface coating on quartz glass for precise formation and positioning of the sample volume
    • Simple cleaning to minimize the risk of sample carry-over
    • A defined angle for folding the sides of the cuvette quarantees reproducible handling
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  • Thermocycler
    • Extremely fast heating and cooling rates
    • Ultimate reduction of evaporation
    • Gradient blocks with SteadySlope® technology
    • Intuitive graphic programming
    • Display to indicate cycler number in a network
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Process Development

Controlled bioreactors can be the key to establish and optimize reproducible cultivation processes and to scale up from T-flasks and plates. The Eppendorf DASbox and DASGIP Parallel Bioreactor Systems offer precisely controlled environments for stem cell cultivation.

  • Mini Bioreactor System
    • Parallel set-up of 4, 8, 12 or more bioreactors
    • Excellent scalability and reproducibility in both microbial and cell culture applications
    • Supports industry standard glass bioreactors (DASbox Mini Bioreactor) as well as BioBLU 0.3 Single-use Vessels
    • Small working volumes save on the amount of cell material, media and supplements required
    • Extremely compact system with a footprint of only 7.5 cm (3 in) benchspace per vessel
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  • Single Use Bioreactors
    • Market leading portfolio of single-use, stirred-tank, rigid-walled vessels available in sizes ranging from 65 mL to 40 L working volumes
    • All BioBLU c vessel sizes available with pitched blade impellers for cell culture applications
    • BioBLU 5p vessels with packed-bed impeller, pre-loaded with Fibra-Cel disks
    • BioBLU 0.3f and 1f vessels with Rushton-type impellers for microbial applications
    • Unique non-invasive pH and DO sensor technology drastically reduces contamination risks (industry standard autoclavable pH sensors available for pH measurement)
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  • Parallel Bioreactor Systems
    • A wide range of industry standard glass and single-use vessels enable reproducible results and scale-up
    • Modular set up allows for individual solutions and flexible upgrading
      of the system
    • Advanced process control and comprehensive DASware software solutions support you in accelerating your process development
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  • Analyzer integration
    • Integration of 3rd party lab devices into bioreactor control units
    • Enables bidirectional OPC interconnectivity, process-triggered feedback control loop and sampling on demand
    • Allows for online calculations and event- or data-driven decisions
    • Users benefit from integration of existing benchtop equipment, better process understanding and optimal process control
    • Integration includes nutrient analyzers & cell counters, biomass monitors, mass spectrometers, automation platforms and autosamplers, HPLC and Raman spectroscopy
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  • Bench-Scale Controller
    • Universal control for both microbial and cell culture applications
    • Interchangeable autoclavable and BioBLU<reg/> single-use vessels
    • Compact design with left- and right-handed orientation for industry leading minimum footprint
    • Control up to eight (8) systems from a single-user interface
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Commercialization

Our single-use bioreactors with working volumes of up to 40 L enable clinical-scale production of stem cells and facilitate validation. New Brunswick BioCommand SCADA Software can be chosen with security, event logs, and audit-trail capabilities to be compatible with the FDA 21 CFR Part 11 requirements.

  • Single-Use Bioreactors
    • Market leading portfolio of single-use, stirred-tank, rigid-walled vessels available in sizes ranging from 65 mL to 40 L working volumes
    • All BioBLU c vessel sizes available with pitched blade impellers for cell culture applications
    • BioBLU 5p vessels with packed-bed impeller, pre-loaded with Fibra-Cel disks
    • BioBLU 0.3f and 1f vessels with Rushton-type impellers for microbial applications
    • Unique non-invasive pH and DO sensor technology drastically reduces contamination risks (industry standard autoclavable pH sensors available for pH measurement)
    more... less...
  • SCADA Software
    • Automatic data logging
    • Remote monitoring and control
    • OPC-compatible
    • Connectivity with current and past New Brunswick bioprocess equipment
    • Advanced packages feature intuitive, user-friendly programming capabilities
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  • Thermocycler
    • Intuitive graphic programming
    • Small footprint
    • Universal block for ultimate flexibility
    • Connect up to 2 Mastercycler nexus eco to your existing unit
    • Available with gradient
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  • Microvolume cuvette
    • Microvolume measuring cell for measuring 1.5 - 10 µL sample volumes
    • Low self-absorption (≤0.05 A @ 260 nm)
    • Hydrophobic surface coating on quartz glass for precise formation and positioning of the sample volume
    • Simple cleaning to minimize the risk of sample carry-over
    • A defined angle for folding the sides of the cuvette quarantees reproducible handling
    more... less...
  • Bench-Scale Controller
    • Universal control for both microbial and cell culture applications
    • Interchangeable autoclavable and BioBLU<reg/> single-use vessels
    • Compact design with left- and right-handed orientation for industry leading minimum footprint
    • Control up to eight (8) systems from a single-user interface
    more... less...