Eppendorf KoreaApplications제약Centrifuge Solutions for Monoclonal Antibody DiscoveryVersatility Solution

Versatility Solution

Centrifuge Solution for antigen concentration, hybridoma technology, PBMC isolation and FACS/Flow cytometry

Purifying peripheral blood mononuclear cells (PBMC), FACS/Flow cytometry and hybridoma technology include multiple centrifugation steps of temperature-sensitive samples in many different consumables. This requires great versatility and an advanced temperature management. In addition, PBMC isolation by Ficoll® gradient centrifugation must be carried out with a minimum of vibration during deceleration (breaking) within reasonable time. Optimized acceleration and deceleration ramps speed up high-yield PBMC isolation up to 88% (see Application Note 372). Refrigerated benchtop Centrifuge 5910 Ri in combination with swing-bucket Rotor S-4xUniversal offer great versatile and an advanced cooling performance for optimal sample protection. The new user management including run records optimally supports GxP documentation.

Increased Efficiency

  • Faster PBMC isolation due to optimized acceleration and deceleration ramps for Ficoll-Paque® density gradient centrifugation.
  • Rotor S-4xUniversal with universal buckets and universal adapters that can spin a selection of tubes, plates and bottles, no need to change rotor, buckets or adapters.
  • Change parameters such as time, temperature and speed in just seconds with the favorites function and quickly access frequently used protocols with the program function. Both functions are directly accessible from the home screen.

Improved Sample Safety

  • End of run counter (time after rotor stopped) to provide information whether PBMCs are still well separated by Ficoll-gradient and thus a maximum yield of viable cells is obtained.
  • Accurate cooling with intelligent Dynamic Compressor Control (DCC) for optimal sample integrity. Unlike other compressors which switch back and forth between 0% and 100% performance output, DCC allows for a constant and smooth temperature regulation. This results in a precise cooling performance and the set temperature is kept stable at all time.
  • In the case of a leakage, you can carry the closed bucket securely to the biosafety cabinet with optional aerosol-tight caps.

 

Better Ergonomics

  • New optional Eppendorf mobile tables lower centrifuge to an ergonomic height for easy loading and unloading of samples and rotors for a more ergonomic work environment.
  • Particularly low access height for easy operation. This not only facilitates easy loading and unloading, it also enables everyone in the lab to easily reach the open centrifuge lid.
  • For ergonomic lid operation Eppendorf designed the soft-touch lid closure system. There is no need to exert unnecessary force when closing the centrifuge lid. In combination with a low opening height you can close the centrifuge with ease.

 

Components

Centrifuge 5910 Ri
  • Super versatile multipurpose centrifuge with high capacity for broad range of consumables, refrigerated for temperature sensitive samples.
  • New swing-bucket standard-rotor S-4xUniversal and its unique universal adapters accommodate bottles, plates and conical tubes.
  • Max. capacity: 4 × 1,000 mL/4 × 5 MTP
Universal Adapter 1
  • For 5.0 mL and 15 mL conical tubes, MTP/DWP
  • Max. capacity: 17 x 5/15 mL tubes or 1 x MTP/DWP
  • Max. speed: 4,324 × g (tubes), 3,237 × g (plates)
Rotor S-4x Universal with buckets
  • Swing-bucket rotor for broad range of high capacity applications in tubes, bottles and plates
  • Aerosol-tight caps optional available.
  • Max. speed: 4,347 × g (4,500 rpm).

 


Universal Adapter 3
  • For 25 mL and 50 mL conical tubes, 250 mL bottles, MTP
  • Max. capacity: 7 x 25/50 mL tubes, 1 x bottles or MTP
  • Max. speed: 4,324 × g (50 mL tubes), 2,604 × g (plates)

Recommended Consumables for Key Methods

Key Method Consumables Advantages

Antigen concentration

  
  • Protein LoBind® Tubes 15 mL, PCR clean
  • Protein LoBind® Tubes 50 mL, PCR clean
  • Conical Tubes 25 mL, screw cap, Starter Pack, PCR clean
  • DWP 96x2000µL White, Protein LoBind®, PCR clean
 		  
  • Minimization of unspecific sample loss with LoBind® consumables
  • Safe sample handling  due to lid tightness
  • Safe centrifugation with high g-force stability & screw Cap
 

Hybridoma technology

  
  • Conical Tubes 50 mL rack, sterile
  • Conical Tubes 15 mL rack, sterile
  • Protein LoBind® Tubes 15 mL, PCR clean
  • Protein LoBind® Tubes 50 mL, PCR clean
  • Conical Tubes 25 mL, SnapTec®, sterile
 		  
  • Reduced risk of culture contamination with high grade sterile purity grade
  • Minimization of unspecific sample loss with LoBind® consumables
 

FACS/Flow cytometry

  
  • Protein LoBind® Tubes 15 mL, PCR clean
  • Protein LoBind® Tubes 50 mL, PCR clean
  • Optional: Conical Tubes 50 mL, amber, sterile
  • Optional: Conical Tubes 25 mL, screw cap, PCR clean
 		  
  • Minimization of sample photodegradation with LoBind® consumables
  • Less degradation of photo-sensitive samples due to amber material
 

PBMC Isolation

  
  • Conical Tubes 50 mL rack, sterile
  • Conical Tubes 15 mL rack, sterile
  • Conical Tubes 25 mL, screw cap, sterile
 		  
  • Reduced risk of culture contamination with high grade sterile purity grade
  • Safe sample handling  due to lid tightness
  • Safe centrifugation with high g-force stability & screw Cap
 
Click to enlarge

Request your individual support

Your own dedicated expert assists you in finding the perfect match for your specific mAb workflow. Get your individual consultation!
 

Contact me