Temperature verification of selected well positions and adjustment of the cycler block temperature if necessary is highly recommended to assure instrument consistency and accuracy. The temperature verification is a very accurate and precise method and meets all documentation and detection requirements in the laboratory according to all national and international standards, e.g.: NIST (USA), DKD/PTB (Germany), UKAS/NPL (UK), COFRAC/LNE (France), NMIA (Australia), MSL (New Zealand). The applicable standards for all other countries apply as well. A full reference list can be found at www.bipm.org/en/cipm-mra/.
Depending on frequency of usage of your Mastercycler ep realplex or environmental conditions a color calibration should be performed on your Mastercycler ep realplex once a year.
Due to the high level of complexity of real-time PCR techniques a PCR validation assay to validate the overall performance of the Mastercycler ep realplex system is recommended or even required in GLP-compliant laboratories.