Is it also possible to adjust the parameters during centrifugation?
Adjustments to the time, rotational speed and temperature (5702 R / 5702 RH only) can be made while the centrifuge is running. To do this, press the SHORT key until the display begins to flash. You can now set the new centrifugation parameters using the TIME and SPEED dials and the TEMP keys. The new centrifugation parameters are taken over after 5 s.
Can the values set for routine applications be secured against unintentional adjustment?
Yes, with the Centrifuges 5702 / 5702 R / 5702 RH it is possible to fix the desired settings. To do this, press the SHORT and the OPEN keys simultaneously for at least 5 s while the centrifuge lid is open. In this way, the dial adjustment is deactivated and "lo on" will appear on the display of the Centrifuge 5702. On the display of the Centrifuges 5702 R / 5702 RH, the fixed setting of the centrifugation parameters will be indicated by an additional pictogram. The set values can now no longer be adjusted, irrespective of whether the centrifuge is running or not. In order to reactivate the dial adjustment, press the SHORT and OPEN keys again for 5 s. "lo of" will appear on the display of the Centrifuge 5702, and the pictogram on the display of the Centrifuges 5702 R / 5702 RH will change. The parameters can now be adjusted again.
How can the set parameters be saved using the program keys?
This is only possible when the centrifuge is not running. First, set the desired program parameters using the TIME and SPEED dials and the TEMP arrow keys. The "at set rpm" function and the slower acceleration and braking ramps can also be saved in the fixed programs, if required. Keep the PROG 1 or PROG 2 key pressed for 2 s until the program key stops flashing and glows continuously. A short signal tone will sound. The program has been saved.
Can the ShortSpin key be programmed?
No, the ShortSpin key cannot be programmed.
What are the maximum values for the acceleration and deceleration times?
The acceleration time to the maximum rotational speed is less than 25 s, the deceleration time from the maximum rotational speed is approx. 25 s.
Is it possible to change the acceleration and braking ramps for specific applications?
Yes, when working with Ficoll density gradients or other sensitive applications (e.g. certain blood and urea centrifugations), slower acceleration and braking ramps can be set for the Centrifuge 5702 / 5702 R / 5702 RH. This allows the centrifuge to accelerate and decelerate smoothly and ensures optimum centrifugation results. Press the SHORT key for more than 5 s while the centrifuge lid is open. The writing on the display changes from "br on" (brake on) to "br OF" (brake off), or "soft" is displayed (5702 R, 5702 RH). The slower acceleration and braking ramps are now activated. If the SHORT key is pressed for less than 5 s while the centrifuge lid is open, the current state is displayed.
Are any rotors for 1.5/ 2.0 mL micro test tubes or microplates available for Centrifuge 5702 / 5702 R / 5702 RH?
The centrifuge has a fixed-angle rotor for 1.5/ 2.0 mL micro test tubes. With the help of adapters, tubes with a volume of 0.2 - 0.6 mL can also be used. A rotor for microplates, however, is not available.
What is the maximum number of 15 mL conical tubes that can be centrifuged in the fixed-angle rotor F-35-30-17 or in the swing-bucket rotor A-4-38?
A maximum of 20 x 15 mL conical tubes can be centrifuged in the fixed-angle rotor F-35-30-17. The maximum capacity of the swing-bucket rotor A-4-38 is 8 x 15 mL conical tubes. Aerosol-tight centrifugation is only possible with 4 x 15 mL conical tubes in the swing-bucket rotor.
Is aerosol-tight centrifugation possible in the Centrifuge 5702 / 5702 R / 5702 RH?
Yes, there are aerosol-tight caps available for the round buckets of the rotor A-4-38. These caps are certified according to Porton Down. All round bucket adapters, except for the adapter 5702 723.002, can be used for aerosol-tight centrifugation. Instead of the adapter 5702 723.002 for two 15 ml Falcon tubes each, the adapter 5702 732.001 for one 15 ml Falcon tube is available for aerosol-tight centrifugation.
How can the rotors and adapters be cleaned?
Rotors, buckets, sleeves and adapters should be cleaned on a monthly basis with a neutral detergent in order to prevent material changes due to residues of the material being centrifuged. Please do not use sodium hypochlorite or chlorous or oxidizing disinfectants and detergents under any circumstances. Disinfection with glutaraldehyde solution is possible. We recommend Cidex® activated glutaraldehyde solution (®: Johnson & Johnson). Plastic adapters and rubber inlays are dishwasher safe.
Are the rotors and adapters autoclavable?
All rotors, buckets, adapters, sleeves and caps are autoclavable for 20 min. at 121°C. The rotors A-4-38 and F-35-30-17 as well as the affiliated buckets and sleeves can even be autoclaved for 2 hrs. at 140°C and 3 bar.
How can the relative centrifugal force (rcf) for the various adapters be exactly determined?
The rcf (g-force) shown on the display is standardized for 15 ml Falcon tubes (rotor: A-4-38). The rcf to be attained at maximum speed for the various adapters are listed in the operating manual. In order to determine a specific rcf for a specific adapter, use the following formula according to DIN 58970:
rcf = 1.118 · 10-5 · n2 · rmax
n: rotational speed in rpm
rmax: max. centrifugation radius in cm
Does the Centrifuge 5702 R/ RH has an energy saving function?
Yes, the ECO shut-off automatically switchs off the compressor after 8 hours of non-use. The device has a stand-by button.
How are the Centrifuges 5804/ 5804 R and 5810/ 5810 R protected during transport? Is there a transport protection device?
No, the Centrifuges 5804/ 5804 R and 5810/ 5810 R do not come with a transport protection device. They are packaged in polystyrene for protection. Because the rotor is not in the centrifuge during transport, there are no movable parts to make a transport protection device necessary.
Can I still change the settings when I have started the Centrifuges 5804/ 5804 R or 5810/ 5810 R?
Yes, the speed, the time and the temperature can be changed even when the centrifuge is running. To do this, please press the SPEED, TIME or TEMP key and change the values by pressing the arrow keys.
How can I recognize in the display of the Centrifuges 5804/ 5804 R or 5810/ 5810 R whether I have selected "rpm" or "rcf"?
For rcf, a "*" is displayed in front of the value. rpm is not indicated by a symbol.
What is the minimum rotational speed (rpm) of the Centrifuge 5804/ 5804 R or 5810/ 5810 R, and in which increments can it be increased?
The minimum rotational speed is 200 rpm, irrespective of the inserted rotor. The minimum rotational speed can be increased in increments of 10 rpm up to 5,000 rpm and then in increments of 100 rpm.
Is it possible to adjust the Centrifuge 5804/ 5804 R or 5810/ 5810 R so that it stops on its own, meaning without active braking?
Yes. Free deceleration of your centrifuge can be achieved by setting the braking level to 0.
"Clean rotor" appears on the display of my centrifuge. Do I now need to remove the rotor from the centrifuge, and, if yes, how?
This display appears every 200 centrifugation runs. For cleaning the rotor and the rotor chamber, the rotor must first be removed from the centrifuge. To do this, loosen the rotor nut by turning it counter-clockwise with the supplied rotor key. Please clean the rotor and rotor chamber only with mild cleaning agents (e.g. 70% alcohol solutions or commercially available alcoholic disinfectants).
The Centrifuges 5804/ 5804 R and 5810/ 5810 R are programmable. What is saved during the programming?
All data is saved that can also be entered manually, such as rotational speed or RCF, maximum radius, run time, acceleration and braking ramps, "at set rpm", temperature.
Is it possible to centrifuge 50 mL and 15 mL tubes together in one rotor?
Yes, it is possible, provided that the rotor is loaded symmetrically. This means that the 50 mL tubes and the 15 mL tubes are positioned opposite one another and are positioned symmetrically. Please use the appropriate adapters for the tubes.
Does Eppendorf offer a rotor for PCR strips for Centrifuges 5804/ 5804 R or 5810/ 5810 R?
The PCR strip rotor F-45-48-PCR is available under the order number 5804 735.005. It can centrifuge up to 6 x 8-well strips at 12,000 rpm / 15,350 x g.
In which rotor is it possible to centrifuge 50 mL DIN glass tubes?
50 mL DIN glass tubes (diameter 34 mm, length 100 mm) can be centrifuged in the adapter for 50 - 75 ml tubes (order number 5804 760.000) of the swing-bucket rotor A-4-44 or adapter 5810 760.003 of swing-bucket rotor A-4-62 . Depending on the length of the tube, the uppermost module of the adapter can be removed for centrifugation of 50 mL DIN glass tubes.
Millipore offers centrifugation filter tubes (Centripreps). Can these tubes be centrifuged in a rotor of the Centrifuge 5804/5804 R or 5810/5810 R?
Yes, for 50 ml Centripreps we offer the adapter 5810 739.004 for the rotor A-4-81.
Into which rotor of the Centrifuge 5804/5804 R or 5810/5810 R do 15 ml and 30 ml Corex tubes fit?
With a rubber adapter (outer diameter 18 mm) available from the tube manufacturer, the 15 ml Corex tubes fit into the adapter 5804 772.008 of the rotor F-34-6-38. With a rubber adapter (outer diameter 29 mm) available from the tube manufacturer, the 30 ml Corex tubes fit into the adapter 5804 774.000 of the rotor F-34-6-38. This rotor can be inserted into the Centrifuge 5804/5804 R or 5810/5810 R.
How big is the g-force difference between the lowest and the highest plate when loading a bucket of the rotor A-2-DWP with four microplates?
The following g-forces are reached with the rotor A-2-DWP:
Lowest plate: 2,250 x g
Highest plate: 1,825 x g
Is there an adapter for slides?
Up to 12 slides can be inserted into the CombiSlide adapter and then centrifuged in the swing-bucket rotors of Centrifuge 5804/ 5804 R or 5810/ 5810 R.
Do 4 microplate buckets (order number 5810 702.003) also fit into the rotor A-4-62 (5810 709.008)?
Yes. It is the same rotor cross. Please note that mixed loading of the rotor with rectangular buckets and MTP buckets is not permitted.
Can rotor A-4-81 or S-4-104 be operated with rectangular buckets and MTP / Flex buckets?
Yes, this so-called mixed loading is possible. Please ensure that the same type of buckets are always used in opposing positions.
Do the adapters for the 0.2/ 0.4/ 0.5/ 0.6 mL tubes, which are designed for the rotors F-45-30-11 and FA-45-30-11, also fit into the rotor F-34-6-38 with the adapter 5804 770.005 (for 1.5/ 2.0 mL tubes)?
Yes, these adapters can also be used in the rotor F-34-6-38 with the adapter 5804 770.005.