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1190 Documents
In which rotors can I centrifuge 0.5 mL micro test tubes?
In which rotors can I centrifuge 0.5 mL micro test tubes?
0.5 mL micro test tubes can be centrifuged in the rotor FA-45-18-11 using an adapter (Order No. International 5425 716.001; North America 022636227).
How do I recognize aerosol-tight rotors?
How do I recognize aerosol-tight rotors?
Firstly, the aerosol-tight rotors can be recognized by their designation abbreviation “FA”, where the letter “A” indicates the aerosol tightness; secondly, we have color-coded the aerosol-tight rotors. All aerosol-tight rotors now have a red rotor lid screw, and a red ring is printed on to the rotor shaft.
Why are the standard rotors in the micro centrifuges 5418, 5418 R, 5424, 5424 R, 5430, and…
Why are the standard rotors in the micro centrifuges 5418, 5418 R, 5424, 5424 R, 5430, and 5430 R aerosol-tight?
At Eppendorf, the safety of the user always has the highest priority. That is why we now fit our centrifuges with aerosol-tight rotors as standard – at no extra charge.
When does the opening of the centrifuge lid take place automatically or manually?
When does the opening of the centrifuge lid take place automatically or manually?
Following the end of a centrifuge run, only the lid of the Centrifuge 5418 is automatically opened in
order to provide quicker access to the sample and to avoid potential heat accumulation. The Centrifuge 5418 R is not equipped with the automatic opening of the centrifuge lid. In order to maintain a consistent temperature in the rotor chamber and in the sample, the centrifuge lid for this device must always be manually opened by pressing the "open" key.
Can the Centrifuge 5418/5418 R be operated in the cooling room at 4° C?
Can the Centrifuge 5418/5418 R be operated in the cooling room at 4° C?
The Centrifuge 5418 can be operated at an ambient temperature in the range from 2° C to 40° C. Operation in the cooling room at a temperature of 4° C is therefore possible. The permitted range of the ambient temperature is between 15 °C and 35 °C for the Centrifuge 5418 R, so that this centrifuge could not be operated in a cooling room at 4 °C.
What is "Fast Temp" (Centrifuge 5418 R)?
What is "Fast Temp" (Centrifuge 5418 R)?
The "Fast Temp" function is a temperature control run of the Centrifuge 5418 R without samples and with a rotor and temperature-specific rotational speed in order to quickly bring the rotor chamber, incl. the rotor, buckets and adapters, to the set nominal temperature. In order that the samples can be centrifuged from the beginning on at the desired temperature, one starts this temperature control run during sample preparation by pressing the "Fast Temp" key. The display shows the current temperature and g force (rcf) or speed (rpm) as well as “FT” as symbol for a “Fast Temp” temperature run. You can find a more detailed description in the operating manual of the Centrifuge 5418/5418 R.
For the 5418 R, when does the continuous cooling of the rotor chamber start?
For the 5418 R, when does the continuous cooling of the rotor chamber start?
The continuous cooling starts with each rotor standstill of the Centrifuge 5418 R in order to maintain the preselected nominal temperature of the rotor chamber not only during centrifugation. To this purpose the following prerequisites must be met:
- The centrifuge is switched on.
- The centrifuge lid is closed.
- The nominal temperature is lower than the ambient temperature.
Continuous cooling is ended by opening the centrifuge lid.
What is the lowest temperature that can be reached with the Centrifuge 5418 R?
What is the lowest temperature that can be reached with the Centrifuge 5418 R?
The lowest temperature of the Centrifuge 5418 R is 0 °C. The device is thereby optimized with the rotor in such a way that at least 4 °C is maintained at the maximum speed.
What is the temperature range of the Centrifuge 5418 R?
What is the temperature range of the Centrifuge 5418 R?
With the Centrifuge 5418 R it is possible to set the temperature within a range from 0 °C to +40 °C.
Does the Centrifuge 5418 R has an energy saving function?
Does the Centrifuge 5418 R has an energy saving function?
Yes, the ECO shut-off automatically switchs off the compressor after 8 hours of non-use.
How did you manage to make the Centrifuge 5424 so quiet?
How did you manage to make the Centrifuge 5424 so quiet?
The Centrifuge 5424 has a soundproof rotor chamber which reduces the noise down to a low level.
Which material are the rotor chamber and the inner centrifuge lid made of?
Which material are the rotor chamber and the inner centrifuge lid made of?
The rotor chamber and the inner centrifuge lid of the Centrifuge 5424 are made of aluminum with a chemically resistant coating.
Can the Centrifuge 5424/ 5424 R be opened in case of a power failure?
Can the Centrifuge 5424/ 5424 R be opened in case of a power failure?
Yes, in case of a power failure, the device can be opened with the help of the rotor key. Before opening the centrifuge, pull the power plug and wait until the rotor stops spinning. The emergency lid release is on the right-hand side of the centrifuge. Remove the plastic cap and insert the rotor key into the nut behind the cap. Turn the rotor key counter-clockwise. The centrifuge lid will be unlocked and can be opened. Then remove the rotor key and replace the plastic cap.
How can the Centrifuge 5424/ 5424 R be cleaned?
How can the Centrifuge 5424/ 5424 R be cleaned?
The Centrifuge 5424/ 5424 R can be cleaned with neutral detergents. For disinfection, we recommend alcohol or alcoholic disinfectants. Make sure that the relevant legal requirements and directives are observed when choosing the disinfection method.
What is the minimum selectable rotational speed with the Centrifuge 5424/ 5424 R?
What is the minimum selectable rotational speed with the Centrifuge 5424/ 5424 R?
The minimum selectable rotational speed is 100 rpm. This is equivalent to an rcf of approx. 1 x g.
In which increments can the rotational speed/g-force be adjusted?
In which increments can the rotational speed/g-force be adjusted?
The rotational speed can be adjusted in increments of 50 rpm, the g-force can be adjusted in increments of 50 x g.
How can the speed display be switched from rotational speed (rpm) to relative centrifugal…
How can the speed display be switched from rotational speed (rpm) to relative centrifugal force (rcf, g-force)?
The display is switched back and forth between rpm and rcf by pressing the "rpm/rcf" key.
In which increments can the time be adjusted?
In which increments can the time be adjusted?
For run time up to 10 min., the increments are 0.5 min. steps, after that it is set in increments of 1 min. up to 9:59 hrs. The function for continuous run mode can be found above 9:59 hrs. and below 0.5 min. The symbol "oo" is displayed to indicate the continuous run.
My Centrifuge 5424/ 5424 R displays "EP". What does that mean?
My Centrifuge 5424/ 5424 R displays "EP". What does that mean?
EP is displayed when the automatic standby function is active. It is not an error code. If the centrifuge remains unused for more than 15 min., the standby function is activated, indicated by "EP" on the display. By using any key or knob or closing the centrifuge lid, the centrifuge is re-activated and ready to use. The letters "EP" were chosen because a lot of Eppendorf products already have this prefix in their name.
Can the fixed-angle rotor FA-45-24-11 be operated without a rotor lid?
Can the fixed-angle rotor FA-45-24-11 be operated without a rotor lid?
The fixed-angle rotor FA-45-24-11 can be used with or without rotor lid. With rotor lid the centrifugation is aerosol-tight and the Centrifuge 5424/ 5424 R is even quieter. The aerosol-tight centrifugation can only be carried out with the rotor lid. Especially when working with pathogenic microorganisms, we recommend the usage of the aerosol-tight lid. When centrifuging filter devices like spin columns or other comparable tubes, the aerosol-tight rotor lid must be used in any case. This protects the centrifuge from damage – e. g. by torn-off tube lids.
Which material is the fixed-angle rotor for micro test tubes made of?
Which material is the fixed-angle rotor for micro test tubes made of?
The fixed-angle rotor is made of massive, anodized aluminum. This material is durable and has got a good chemical resistance. The rotor lid is made of the same material.
What is the benefit of the so-called coated rotors?
What is the benefit of the so-called coated rotors?
The coated rotors are available for the Centrifuges 5424/ 5424 R and 5430/ 5430 R. The special coating provides an enhanced chemical resistance against phenol, acetonitrile, DMSO, acetone, trichloroacetic acid, acetic acid and sodium hypochlorite. It is a PTFE coating which is applied on top of the actual anodic coating.
Which tubes can be used in the Centrifuge 5424/5424 R?
Which tubes can be used in the Centrifuge 5424/5424 R?
With the Centrifuge 5424/ 5424 R, 1.5 mL and 2.0 mL Eppendorf® tubes can be spun in the rotors FA-45-24-11 und FA-45-24-11-Special. With the help of adapters, the centrifugation of 0.2 mL PCR tubes, 0.6 mL Microtainer, 0.5 mL and 0.4 mL micro tubes is also possible. Even so-called spin columns can be centrifuged in the rotor F-45-18-11-Kit (e.g. Qiagen, Macherey-Nagel, Vivascience). For centrifugation of spin columns, the rotor lid must be used. In the rotor F-45-32-5-PCR, 0.2 mL PCR strips can be used directly.
For which applications can I use the Centrifuge 5424/5424 R?
For which applications can I use the Centrifuge 5424/5424 R?
The four different rotors of the Centrifuge 5424/ 5424 R allow an exceptionally broad variety of applications. Up to 24 micro test tubes can be centrifuged at 21,130 x g/ 15,000 rpm (5424: 120 V, 5424 R: 230 V, 120 V, 100 V) or 20,238 x g / 14,680 rpm (5424: 230 V, 100 V). It can be used with tubes from 0.2 up to 2.0 mL. The potential applications include:
- Isolation of DNA, RNA and proteins
- Spin columns
- Aerosol-tight centrifugation of infectious samples
- Pelletizing of cells (bacteria, eukaryotic cells) up to 2.0 mL
- Spinning down of PCR mixtures in tubes and strips
- Working with aggressive substances (e.g. phenol, chloroform)
In which rotor can I use spin columns (centrifugation columns)?
In which rotor can I use spin columns (centrifugation columns)?
Spin columns or small filtration columns, for example for centrifugation of DNA or RNA, can be used in all rotors for 1.5/2.0 mL micro test tubes, e.g. the aerosol-tight 24-place rotor (FA-45-24- 11) and the aerosol-tight, special coated 24-place rotor (FA-45-24-11-Special). In particular, however, the kit rotor F-45-18-11-Kit is specially suitable for such spin columns, as open tube lids are supported by the raised edge of this rotor type and are no longer torn off (e.g. during elution of
the sample from the column into a micro test tube).
In which rotors can I centrifuge 0.2 mL PCR tubes?
In which rotors can I centrifuge 0.2 mL PCR tubes?
Up to 32 0.2 mL PCR tubes (or four 8-tube PCR strips) can be centrifuged in the PCR rotor F-45-32-5-PCR. Furthermore, 0.2 mL PCR tubes can be used in each rotor for 1.5/2.0 mL micro test tubes (FA-45-24-11, FA-45-24-11-Special) using an adapter (Order No. International 5425 715.005; North America 022636260).
In which rotors can I centrifuge 0.5 mL micro test tubes?
In which rotors can I centrifuge 0.5 mL micro test tubes?
0.5 mL micro test tubes can be centrifuged in all rotors for 1.5/2.0 mL micro test tubes (FA-45-24-11, FA-45-24-11-Special) using an adapter (Order No. International 5425 716.001; North America 022636227).
What is the SOFT function?
What is the SOFT function?
The SOFT function of the Centrifuge 5424/ 5424 R allows slower and gentler acceleration and braking compared to the normal acceleration and braking times. As a result, delicate samples are not stressed unnecessarily. In addition, the risk of the pellets being shaken up again during braking is minimized.
When does the opening of the centrifuge lid take place automatically or manually?
When does the opening of the centrifuge lid take place automatically or manually?
Following the end of a centrifuge run, only the lid of the Centrifuge 5424 is automatically opened in order to provide quicker access to the sample and to avoid potential heat accumulation. When working with radioactive material, this function should be deactivated in the device menu and the centrifuge lid manually opened upon conclusion of the centrifugation run. The Centrifuge 5424 R does not come equipped with the option of automatic or manual opening of the centrifuge lid. In order to maintain a consistent temperature in the rotor chamber and in the sample, the centrifuge lid for this device must always be manually opened by pressing the "open" key.
Can I display the centrifugal force (rcf, g force) automatically with the Centrifuge 5424…
Can I display the centrifugal force (rcf, g force) automatically with the Centrifuge 5424 /5424 R?
Yes. It is possible to display the centrifugation speed in both rpm and rcf on all Eppendorf centrifuges. In line with the new operating concept of the centrifuges 5418, 5424, 5424 R, 5430 and 5430 R, the units now have a separate rpm/rcf conversion button.