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Lysate clarification in nucleic acid purification protocols for target antigen production and antibody humanization include high speed centrifugation runs with up to 20,000 x g. High numbers of temperature-sensitive samples with up to 50 mL need to be processed. Each protocol has multiple centrifugation steps interrupted by pipetting and vessel changes. Refrigerated benchtop Centrifuge 5910 Ri in combination with low-weight aluminum rotors offer advanced cooling performance for optimal sample protection and reproducibility. The new user management including run records optimally supports GxP documentation.

Increased Efficiency

  • Change parameters such as time, temperature and speed in just seconds with the favorites function.
  • Quickly access frequently used protocols for increased efficiency and repeatable results with the program function.

Improved Sample Safety

  • Accurate cooling with intelligent Dynamic Compressor Control (DCC) for optimal sample integrity. Unlike other compressors which switch back and forth between 0% and 100% performance output, DCC allows for a constant and smooth temperature regulation. This results in a precise cooling performance and the set temperature is kept stable at all time.
  • Use pre defined protocols for repeatable results with the program function.
  • Information whether pellet is instable and run has to be repeated with end of run counter (time after rotor stopped is displayed).

Better Ergonomics

  • For ergonomic lid operation Eppendorf designed the soft-touch lid closure system. There is no need to exert unnecessary force when closing the centrifuge lid. In combination with a low opening height you can close the centrifuge with ease.
  • QuickLock rotors close with only 1/4 of a turn. This not only saves you time during every day use, it also takes repetitive stress off your wrist.
  • Particularly low access height for easy operation. This not only facilitates easy loading and unloading, it also enables everyone in the lab to easily reach the open centrifuge lid.

Components

Centrifuge 5910 Ri

  • Super versatile multipurpose centrifuge with high capacity for broad range of consumables, refrigerated for temperature sensitive samples.
  • Fixed-angle rotors for high-speed molecular biology applications in vessels from 0.2 mL to 250 mL.
  • Max. capacity: 4 × 1,000 mL/4 × 5 MTP.

Rotor FA-6x50

  • Fixed-angle rotor for molecular biology applications in conical tubes up to 6 x 50 mL
  • Aerosol-tight with Eppendorf QuickLock lid.
  • Max. speed: 20,130 × g (12,100 rpm).

Recommended Consumables for Key Methods

Key Method Consumables Advantages
 
  • Plasmid Construction
  • Supernatant/Lysate clarification
  • Humanization (Plasmid construction, big volume)
 
 
  • Conical Tubes 50 mL rack (sterile)
  • Conical Tubes 25 mL, SnapTec®, Starter Pack, PCR clean
 
 
  • Safe handling of bigger volumes (25-50mL) due to lid tightness
  • Safe centrifugation with high g-force stability & screw Cap
  • Easy handling of bigger volumes (25-50mL) with  SnapTec® lid
  • Minimization of DNA/RNA Sample degradation with PCR Clean purity grade
  • Minimization of unspecific sample loss with LoBind® consumables
 
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