Sample Preparation

Heating, cooling, and mixing

Many protocols for COVID-19 applications require dedicated temperature incubation steps before PCR runs. Specific temperatures, eventually mixing of reagents for a better performance – the more systematic, the more specific and the more reproducible are your results.
A reliable sample handling process is mandatory for any further downstream application. Without knowing where your samples are located, what is filled in which vial and what was done with these samples when and by whom – what shall you do?
Learn more about the sample preparation process:


Improve Your Assay Results by Dry Incubation and Mixing of Your Samples

You need everything?

The Eppendorf ThermoMixer® C combines excellent mixing performance with superior temperature control to ensure complete, dependable, and reproducible test results. Improve your assay results by mixing and incubating samples at the same time. The Eppendorf ThermoMixer C convinces users with its quick and easy-to-exchange SmartBlocks for all common lab vessels, new and improved program functions, and high level of stability for every mixing frequency. There is almost no limit to the variety of applications waiting for you!
 

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Facing irregular mixing results?

Reliable sample mixing is far beyond simple mixing, especially when talking about microliter volumes.

Versatility needed?

Watch the video to learn about the mixer versatility.


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One Will Fit to Your Needs

Using different vessel sizes within your lab workflow?


Eppendorf consumables are made of highest quality, virgin polypropylene (PP) free of biocides, plasticizers and latex. Production is performed without slip agents to minimize leachables within the consumables.

Different purity grades like sterile (incl. pyrogen-free, RNase-free, DNase-free, human and bacterial DNA-free), PCR clean, Forensic DNA Grade, or Biopur® are available. Independent external purity tests are confirmed by certification.

 

Perfect fit of your vials in thermoblock


Flexibility has never been this easy. Eppendorf offers a broad range of Eppendorf SmartBlocks for tubes from 0.2 mL to 50 mL as well as plates (MTP, DWP, and PCR plates 96 / 384) to use with Eppendorf ThermoMixer® C and ThermoStat™ C.
All Eppendorf SmartBlocks are equipped with the outstanding Eppendorf QuickRelease™ system that makes the block exchange super fast and easy.
 

No tool available?

The Eppendorf SmartBlocks can easily be exchanged. Just press the lever on the front of the block, and the block can be removed – no tools are needed, done in seconds The Eppendorf SmartBlocks can easily be exchanged. Just press the lever on the front of the block, and the block can be removed – no tools are needed, done in seconds.

 

 

 

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Incubation, Mixing – What Else is Needed?

Heating, cooling, and mixing within the Eppendorf ThermoMixer® is the core function. Still, there are accessories which help to make scientific life even easier:

Parallel transfer of vessels to enable an identical incubation of all samples, avoiding condensation within the tubes, more than one temperature for quick temperature changes to e.g. stop an enzymatic reaction, or optimal conditions for PCR set-ups.

Tired of condensation
in your tubes?

Prevent condensation within the tube lid by using the ThermoTop – plus, sample concentrations are consistent for the incubation time.

 

Missing a second temperature for an incubation at 95 °C?

Up to 12 vessels (1.5 mL) can be heated up for a higher throughput and higher flexibility in your lab. The independent heating function for the second temperature enables a parallel usage of an Eppendorf SmartBlock and the SmartExtender.

Annoyed by ice buckets
at your bench?

The PCR-Cooler is a perfect tool for keeping your samples in 0.2 mL/ 0.5 mL PCR tubes and 96-well PCR plates cold throughout your PCR setup.

 


Keep Track of Your Samples and Processes

When handling your samples, it is vital to keep them safe and accessible. Accurate sample labeling by barcoding is only the beginning, you still need to maintain detailed documentation of your working steps.

Safely collect all information about your valuable samples and lab processes in one convenient location. Both barcoded vessels and manually-labeled vessels can be integrated.

Easily track samples and data with eLABJournal®, the  electronic lab notebook and integrated sample management software.

Sample ID needed?

Easy and fast sample recognition for safe sample ID by SafeCode 3-level coding:

  • 1D barcode (side)
  • Clear coding (side)
  • 2D data matrix code

Different volumes needed?

CryoStorage vials with SafeCode are available from 0.5 to 4.0 mL in different purity grades

Paperwork required?

Keep important documentation like certificates easily accessible for each individual vessel

Go digital:

Document your working steps, save run protocols, and add further background information to your sample.

 

 

Data security mandatory?

eLAB works in accordance to the strict requirements of the ISO/IEC 27001:2013 standard

Documentation duties to be fulfilled?

21 CFR part 11 compliance for documentation security


 




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From Mixing to Shaking – Keep the Performance

Mixing versus shaking – the difference is: The volume Independent from the volume of your sample, the reliable mixing of the liquid is crucial for a successful result of your experiment.

This reliability symbolizes the Eppendorf ThermoMixer® family for small volumes whereas the Innova® Shaker family provides you the same reliability for bigger sample volumes. Change the volume – and keep the performance

 

 

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Fitness for Your Mixer

An annual inspection and maintenance of your mixing devices by an Eppendorf certified service technician is highly recommended to ensure a safe working environment.

Make use of the new Global Mixer Service Agreements from cost-efficient to all-inclusive care packages for easier service process and maximized uptime.

 

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Further reading

Sample handling solutions. Discover answers behind the products…

 

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It’s about you – Improve ergonomics in your lab, learn more about PhysioCare

 

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