Impact on the systematic accuracy of a pipette with liquid densities deviating from the density of water. When using dense liquids the pipette needs to be adjusted for proper volume delivery.
Liquid temperatures other than 22 °C lead to systematic measurement deviations if forward pipetting technique is performed. Therefore another pipetting technique like reverse pipetting or a different pipette type like a direct replacement system should be considered.
Pipettes are calibrated to an altitude of 0 m sea level. Different altitudes lead to higher systematic errors and can only be beaten by a permanent adjustment of the pipette.
If you want to read more about the influence of physical parameters on the dispensed volume of air-cushion pipette, please download our Userguide here!