Novel preprogrammed methods for DNA or RNA purification from blood, tissue or from viruses

03/05/19

The majority of life science labs which need to purify genomic DNA from blood or tissue, or isolate nucleic acid from viruses, use specialized kits, as this simplifies the procedure substantially. Further, using kits for nucleic acid purification can improve DNA and RNA yields, and enhances the quality of the final extracts. Switching from one kit to another usually requires adaptation of the process. The same is true when working with an automated liquid handler.

Those of you who are currently using the MagSep kits from Eppendorf can switch to MACHEREY-NAGEL® NucleoMag® kits as the MagSep Kit family will soon be discontinued (table 1).

We optimized the respective automated methods for the epMotion 5073m liquid handling platform as ready-to-run methods, and described the automated procedure in short protocols in order to support your transition.

In these short protocols we demonstrated that the new NucleoMag kits from MACHERY-NAGEL deliver the same, or even a higher yield and quality of the final extracts than the MagSep kits did. Additionally, we prepared a preprogrammed method for your epMotion 5073m for each kit. The automated methods for the NucleoMag kits can be downloaded from the section on the upper right for the epBlue™ software versions 40.5 and 40.6. Please choose the preprogrammed method according to the software version installed on your epMotion 5073m.

You will be able to use the method together with the NucleoMag kits directly without further adaption. There are just a couple of adaptations necessary if you own the epMotion 5075m.
If you need support with using your epMotion 5075m with these methods, or you have other questions please contact your local Eppendorf sales organization (redirect zu local teams) or your automation specialist.

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