If you follow a given protocol, make sure to use the same type of rotor and apply the given relative centrifugal force (rcf or g × force) as well as the same temperature and running time. In general, the following major parameters have to be determined to achieve a successful centrifugation run:
- A: Type of sample
- B: Type of vessel
- C: Type of centrifuge
- D: Type of rotor
- E: Necessary relative centrifugal force
- F: Necessary temperature during centrifugation
Relative centrifugal force (rcf) influences running time and the quality of pellets and is determined by the type of sample, tube, rotor, speed, and centrifuge. When using rotors with different radii to spin a particular tube, please use the same rcf instead of the same revolutions per minute (rpm). To convert rpm and rcf you will need the maximum rotor radius and either the rpm value or the rcf value.