Basic of Cell Culture Training
Students and researchers who wish to begin or improve cell culture techniques and workflow.
Objective of seminar:
Participants will learn fundamental techniques covering a typical cell culture workflow, starting from culture initiation and its maintenance to evaluation of cell growth profile and cell-based assay. During the course, participants will gain practical experience on aseptic techniques and routine cell culture methods as well as acquiring the essential knowledge of experimental design & troubleshooting in cell culture lab.
Content of seminar:
> Cell culture overview
> Contamination in cell culture and prevention
> Aseptic techniques
> Cell growth assessment
> Cell viability assay
> Experimental design
> Cell thawing and culturing techniques
> Cell passaging and cryopreservation
> Cell seeding
> Cell-based assay set-up and results