To harness the potential of human pluripotent stem cells (hPSCs), abundant provision of their differentiated progenies is required. During this webinar we present how hPSC expansion as matrix-independent aggregates in suspension culture (3D) was successfully combined with cardiomyogenic differentiation. A multi-well screening approach was scaled-up to stirred-tank bioreactors applying controlled feeding strategies (Batch and Cyclic Perfusion) followed by differentiation.
Optimized conditions in 100 mL cultivations enabled the production of 40 million cardiomyocytes (>80% purity), the majority of which displayed ventricular-like action potentials and were directly applicable for bioartificial cardiac tissue formation, a potential strategy for heart repair, drug discovery and safety pharmacology.
- Learn about challenges in up-scaling hPSC suspension cultures and the latest achievements using stirred-tank bioreactors.
- Understand the role of bioreactor design for cell aggregate formation and
- Learn about tools and process parameters for controlled cultivation of stem cell cultures.
This webinar took place on June 9, 2015.