Can you tell me the depth and the diameter of the bores of the exchangeable thermoblocks?
The specification can be found within Userguide 001: "Applications for Thermomixer comfort, Thermomixer compact and ThermoStat plus" (Please insert weblink here)
Does the thermoblock for microtest plates have a lid?
Yes. When the exchangeable thermoblock for MTP block is ordered, the lid is delivered in the same packaging. It can also be ordered separately (order no.: 5363 000.217).
Can 24-well cultivation plates be used in the exchangeable thermoblock for MTPs and deepwell plates?
No. The thermoblock for MTP is designed for the globally recognized standard microplate with dimensions of 127 x 86 mm. The microtiter plates for cultures have larger dimensions (132 x 88 mm) and therefore do not fit in this block.
Which slides fit into the exchangeable thermoblock for 4 slides (Order no. 5368 000.010)?
The exchangeable thermoblock 'slides' can be used for all glass slides of 75-76.5 mm length, 25-26 mm width and a height of up to 1.2 mm. This thermoblock is well suited for all types of hybridization reactions such as microarray hybridization or fluorescence in situ hybridization (FISH). Application notes descibing the use of thermoblock 'slides' for those kind of applications can be found on our homepage (Please insert web-link here).
Is the exchangeable thermoblock for slides compatible with the Eppendorf DualChip Microarrays?
No. For the DualChip Microarrays, an exchangeable thermoblock is required that applies additional pressure to the hybridization frames. This reduces potential evaporation to a minimum.
Can the IsoRack be centrifuged?
Please note that we offer a special IsoRack adapter for our 5810/R (swing-bucket rotor type A-4-81, flex buckets). In addition, the IsoRacks can also be centrifuged in the A-2-DWP and in the A-4-62-MTP. In this case, centrifugation is limited to a max. rotational speed of 3,700 rpm (max. 1,000 centrifugations).
Are the cool packs (Isopack) of the IsoTherm-System available individually?
Can the IsoSafe insulation box of the IsoTherm-System be autoclaved?
What are IsoPacks made of?
They are made of polyethylene and filled with a special, non-toxic liquid. The bottom is covered with an aluminum foil.
Are the IsoPacks resistant to sodium hypochloride (NaOCl)?
NaOCl attacks the aluminum foil (for both packs sizes) slightly. Over a period of eight hours or more, this may lead to damage. The IsoPack polyethylene housing is chemically resistant to NaOCl. Even if a leak occurs, the liquid in the IsoPack is non-toxic.
What are IsoRacks made of?
They are made of polycarbonate.
How long do IsoPacks (white, blue) maintain their temperature?
"If IsoPacks (in an IsoRack) are sealed in an insulating box (IsoSafe), a temperature of 0 °C (in the white packs) can be maintained for at least 6 hours and –21 °C (in the blue packs) for > 3 hours.
If the IsoPacks are in an open insulating box, 0 °C can be maintained for roughly 4 hours and –21 °C for 1–2 hours.
If the IsoPacks/IsoRacks are not placed in an insulating box, a temperature of 0 °C can be held for 1.5–3 hours and –21 °C can be held for 0.5–1 hour."
Can the IsoSafe be cleaned in a dishwasher?
At which speed is vortexing possible?
You can use either the touch vortexer at 3,500 rpm, or vortexing can be set to continuous by setting the mixing rate and mixing period to a range between 300 and 3,000 rpm. In continuous vortexing mode vortexing at mixing frequencies between 2,000 rpm and 3,000 rpm may occasionally activate the automatic imbalance detector.
During continuous vortexing the speed dropped suddenly and "TOO FAST" appeared in the display, why?
MixMate's automatic imbalance sensor, which prevents plates and tubes from jumping out during the mixing process, may be occasionally initiated during continuous vortexing at speeds between 2,000 and 3,000 rpm. Safety when mixing plates is of utmost priority, therefore this feature cannot be technically altered.
MixMate suddenly reduced my mixing rate and "TOO FAST" appeared in the display, why?
MixMate's automatic imbalance sensor determined irregularities during the mixing process and, for safety reasons reduced the mixing rate to prevent the plate or tubes from jumping out of the holder. This may be attributed to one or more of the following This may be attributed to one or more of the following reasons:
- Plates are not correctly seated, or they do not comply with the SBS format
- tube holders have not been properly inserted, or they have been unequally loaded
- the mixing rate has been set too high for the weight of the mixing material
- In continuous vortexing mode vortexing at frequencies between 2,000 rpm and 3,000 rpm may occasionally activate the automatic imbalance detector.
My tube holders rattle when I use MixMate, why?
The tube holders may not have been properly inserted into the Universal Holder. Check to make sure that the lugs on the rear side of the tube holder are properly latched into the holes of the Universal Holder.
I would like to check the accuracy of the mixing rate. How do I do it?
We recommend using a calibrated stroboscope or a calibrated flash lamp with a systematic error of <- 0.1 %.
Can MixMate be used in automated systems or integrate into an automated pipetting system?
No, because MixMate is not equipped with an interface.
Can I program MixMate to run different mixing steps sequentially?
No. MixMate is not programmable.
Can MixMate be used as a magnetic stirrer?
Is MixMate able to heat or cool samples?
No, MixMate is a mixing device. For tempering (heating, cooling) of plates or tubes we recommend the Eppendorf Thermomixers or Eppendorf ThermoStat plus.
Can MixMate be operated in an incubator at 37°C?
Yes, MixMate can be operated at ambient temperatures of 2 – 40°C and a relative air humidity of 10 – 75 %.
Can MixMate be operated in a cold room at 4°C?
Yes, MixMate can be operated at ambient temperatures of 2 – 40°C and a relative air humidity of 10 – 75 %. A note of caution: Condensate formation may damage electronic components! After using MixMate in a cold room, wait one hour before reconnecting the device to the power supply.
Will using MixMate heat my sample?
MixMate's ventilation concept enables the installed fans to take in cool air from above while dissipating the heated air from the bottom of the device. Thus, waste heat does not come into contact with the sample material. Internal tests have shown that even after 3 hours of continuous operation at 3,000 rpm, a sample will be heated by no more than max. 2-3°C.
Can I switch off the acoustic signal during operation, or at least turn down the volume?
You can either deactivate the signal tone (VOL0) or choose between three different volume settings (VOL1 – VOL3). To do so use the arrow key to select "M-1 VOL" in the menu. After pressing the "Menu/Enter" button you can edit the settings.
Can I use the Eppendorf IsoRack (of the IsoTherm-System) on MixMate?
Yes, the Eppendorf IsoRacks for 1.5/2.0 ml and 0.5 ml micro test tubes can be used in combination with the 1.5/2.0 ml or 0.5 ml tube holders.
Can I control MixMate using a computer?
No. MixMate is not equipped with an interface, therefore it cannot be controlled by external devices.
Can I order the tube holders individually as replacement parts?
Yes, to do so please contact your local Eppendorf dealer. The catalog numbers for the tube holders are:
- Tube Holder PCR 96: 5353 040.113 (Eppendorf North America: 022674005)
- Tube Holder 0.5 ml: 5353 040.121 (Eppendorf North America: 022674021)
- Tube Holder 1.5/2.0 ml: 5353 040.130 (Eppendorf North America: 022674048)
Can I order the vortex mat as a replacement part?
Yes, to do so please contact your local Eppendorf dealer. The order number for the vortex mat is: 5353 863.004 (Eppendorf North America: 022674105)