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Classic Cell Culture Solution

Centrifuge Solution for the establishment of stable line

In a final step of the mAb drug discovery workflow a stable cell line is established that can express high amounts of humanized monoclonal antibody long-term. Cultivation and selection of stable cell lines require multiple centrifugation steps. Centrifuge 5810 in combination with Rotor S-4-104 offers an economical solution with an excellent footprint to capacity ratio. This setup is ideal for standard cell culture applications including cell harvesting in 15 mL or 50 mL conical tubes.

Increased Efficiency

  • Excellent footprint to capacity ratio to save precious benchtop space (smallest 3 Liter centrifuge in the market)

Improved Sample Safety

  • Aerosol-tight bucket caps to reduce risk of sample-/cross contamination with improved handling for easy one-hand operation optional available.
  • Newly inserted rotors are detected automatically to limit speed to the maximum permissible value and guarantee correct speed to g-force conversion.

Better Ergonomics

  • Soft-touch lid closure system for ergonomic lid operation. There is no need to exert unnecessary force when closing the centrifuge lid. In combination with a low opening height you can close the centrifuge with ease.
  • Low lid opening height for use under shelves.
  • New optional Eppendorf mobile tables lower centrifuge to an ergonomic height for easy loading and unloading of samples and rotors for a more ergonomic work environment.

Components

Centrifuge 5810
  • Compact multipurpose centrifuge with medium capacity, non-refrigerated
  • 12 different rotors available
  • Max. capacity: 56 x 15 mL/28 x 50 mL/4 × 4 MTP
Adapter 5/15ml
  • For Eppendorf Tubes® 5.0 mL and 15 mL conical tubes
  • Max. capacity: 14 x 5 mL or 15 mL
  • Max. speed: 3,197 × g
Rotor S-4-104 with 4 × 750mL round buckets
  • Medium capacity swing-bucket rotor
  • Max. capacity: 4 x 750 mL
  • Max. speed: 3,214 × g (3,900 rpm)

Adapter 50ml
  • For 50 mL conical tubes
  • Max. capacity: 7 x 50 mL
  • Max. speed: 3,180 × g

Recommended Consumables for Key Methods

Recommended Consumables

Key Method Consumables Advantages

Establishment of stable cell line (splitting, harvesting, seeding)

  
  • Conical Tubes 15ml bulk, sterile
  • Conical Tubes 50ml bulk, sterile
  • Conical Tubes 25 mL, SnapTec®, sterile
  • Conical Tubes 25 mL, screw cap, sterile
  • Conical Tubes SnapTec®® 50 mL PCR clean, 240 tubes, 8x30 tubes/bag
 		  
  • Reduced risk of culture contamination with high grade sterile purity grade
  • Safe centrifugation due to high g-force stability and lid tightness
  • Easy sample handling with SnapTec® lid
 
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