Are Eppendorf Micro- and Multipurpose centrifuges equipped with an imbalance switch-off function?

Yes, Micro- and Multipurpose centrifuges are equipped with an imbalance detection. As soon as an imbalance is detectetd, an error message occurs on the display and the run is stopped automatically.

What are the noise levels of Eppendorf Microcentrifuges?

The noise level of Eppendorf Microcentrifuges ranges from 49 dB(A) to 58 dB(A). What does that mean? Here are some examples of every day life noises: 45 db(A) - a quiet library or soft conversation at home 50 db(A) - the hum of a refrigerator 58 db(A) - dishwasher running in the next room Eppendorf Microcentrifuges have a very moderate noise level though. Please find the exact numbers in the section technical data on the respective homepages or in the respective Operating Manuals.

How can Eppendorf Micro- and Multipurpose centrifuges be opened in case of a power failure?

Each Eppendorf centrifuge is equipped with an emergency lid release. The centrifuge lid can be opened e.g. with the support of the rotor key. Detailed description of this topic is listed in the respective Operating Manual which can be downloaded from the Eppendorf homepage.

What is the minimum selectable rotational speed of an Eppendorf Microcentrifuge?

The minimum selectable speed is 100 rpm or 1 rcf (g-force).

Can the fixed-angle rotors of Eppendorf Microcentrifuges be operated without rotor lids?

The fixed-angle rotor FA-45-18-11 can be used with or without rotor lid. With rotor lid the centrifugation is aerosol-tight and the Centrifuge 5418/5418 R is even quieter. The aerosol-tight centrifugation can only be carried out with the rotor lid. Especially when working with pathogenic microorganisms, we recommend the usage of the aerosol-tight lid. When centrifuging filter devices like spin columns or other comparable tubes, the aerosol-tight rotor lid must be used in any case. This protects the centrifuge from damage – e. g. by torn-off tube lids.

What is the FastTemp function of refrigerated Eppendorf Micro- and Multipurpose centrifuges?

The FastTemp function is a specific temperature control run which was developed to reach the set temperature as quickly as possible. This temperature control run can already be started during sample preparation by pressing the FastTemp button. This enables a centrifugation at the desired temperature directly after sample preparation without time loss. Please find more detailed information of the FastTemp function in the respective operating manuals of Eppendorf Micro- and Multipurpose centrifuges on the Eppendorf homepage.

How can Eppendorf Micro- and Multipurpose centrifuges be cleaned?

For cleaning and disinfection of Eppendorf centrifuges you will find detailed information in the respective operating manuals which can be found in the section manuals on this page. Furthermore we recommend the following papers which can be found in the section Notes&Papers on the Eppendorf homepage. - Whitepaper 014 - Maintenance Poster - Logfile for Routine Maintenance

Can I use both - rpm and g-force (rcf) - to set the speed parameter of an Eppendorf Microcentrifuge?

Yes. Each Eppendorf Microcentrifuge has the possibility to use rpm or g-force (rcf) to set the speed. It is equipped with a special button to easily change between rpm and rcf. For the centrifuges Minispin / Minispin plus a combination of buttons have to be pressed. Please refer to the devices Operating Manuals for more detailed information. Information available on the product page on eppendorf.com.

Is it possible to smooth the acceleration or braking behaviour of an Eppendorf Microcentrifuge for the centrifugation of gradients or soft pellets?

Yes. The SOFT function of Eppendorf Microcentrifuges 5425R, 5427R, 5430/R was implemented to realise smooth acceleration and braking. It can be used for the centrifugation of gradients or soft pellets. With Microcentrifuges 5420 and 5425 one could choose between accelerating ramps 1 - 9 and braking ramps 0 - 9. The smaller the ramp the smoother the acceleration / braking.

What is the advantage of the automatic lid release of Eppendorf Microcentrifuges?

The automatic lid release is a feature of Eppendorf's air cooled Microcentrifuges. It can be switched On and Off via the centrifuges operating menu. If it is activated, the centrifuge lid opens automatically once the centrifugation run is finished. This allows quicker access to the samples and avoids possible heat build-up. In addition, the open lid is a clear signal - even from a distance - that the centrifugation run has ended. This function has not been implemented in the refrigerated microcentrifuges from Eppendorf, as a set temperature can be set there which should also be maintained after the end of a centrifuge run.

What tubes can be centrifuged with Eppendorf Microcentrifuge 5430/ 5430 R?

Due to the 12 different rotors and adapters, a variety of tubes can be centrifuged with the Centrifuge 5427R: • Micro test tubes (0.5 - 5.0 mL) • Conical tubes (15 mL, 50 mL) • blood collection tubes • PCR tubes and strips and plates • Spin columns / Filter inserts • Cryo tubes • HPLC tubes You will find a detailed list of usable tubes in the centrifuges operating manual (chapter technical data) which can be downloaded from the Eppendorf homepage.

What tubes do you recommend for centrifugation at 30,000 x g?

The specifications of the tube manufacturers on the centrifugation resistance must generally be taken into consideration for the centrifugation of tubes of all kinds. This applies in particular for centrifugation at 30,000 x g, as only very few micro test tubes can withstand these extreme gravitational forces to date without breaking. The following tubes can, for example, be centrifuged at 30,000 x g: • Eppendorf Safe-Lock Tubes 0.5 mL • Eppendorf Safe-Lock Tubes 1.5 mL • Eppendorf Tubes 3810X 1.5 mL

For which applications can I use Eppendorf Microcentrifuge 5430/5430R?

Centrifuge 5430/5430R allow a broad variety of applications. Up to 24 micro test tubes can be centrifuged at 30,130 xg / 48 micro test tubes at 18,210 xg. It can be used with tubes from 0.2 up to 50 mL and micro plates. Possible applications include: - Isolation of DNA, RNA and proteins - Spin columns / Filter inserts / Cryo tubes - Aerosol-tight centrifugation of infectious samples - Pelletizing of cells (bacteria, eukaryotic cells) up to 50 mL - Spin down of PCR mixtures in tubes or strips - Centrifugation of plates

What is the FastTemp pro function of Eppendorf centrifuges?

With the FastTemp pro function, it is possible to start the FastTemp temperature control run automatically at a preset time. This means that the centrifuge is already precooled to the preselected temperature at the selected time x (e.g. in the morning at the start of work). This feature is implemented in Eppendorf Microcentrifuges 5430/5430R and Eppendorf Multipurpose Centrifuges 5910R and 5920R. Eppendorf homepage Please find detailed information in the respective devices operating manual on the Eppendorf homepage.

What kind of tubes can be centrifuged in Eppendorf Microcentrifuge 5430/5430R?

Due to the 12 different rotors and adapters, a variety of tubes can be centrifuged with the Centrifuge 5427R: • Micro test tubes (0.5 - 5.0 mL) • Conical tubes (15 mL, 50 mL) • blood collection tubes • PCR tubes and strips and plates • Spin columns / Filter inserts • Cryo tubes • HPLC tubes You will find a detailed list of usable tubes in the centrifuges operating manual (chapter technical data) which can be downloaded from the Eppendorf homepage.

What is the Short Spin function of Eppendorf Micro- and Multipurpose centrifuges?

The Short Spin function was developed to be able to perform a quick centrifuge run without having to set parameters. To start a short spin centrifugation, simply press the short key. Centrifugation will continue for as long as it is pressed. Please find detailed information on this function in the respective devices manual which can be found on their Eppendorf hompages.

How do I recognize aerosol-tight rotors?

Firstly, the aerosol-tight rotors can be recognized by their designation abbreviation “FA” or “AT”; secondly, Eppendorf has color-coded the aerosol-tight rotors. All aerosol-tight rotors have a red rotor lid screw, and a red ring is printed on to the rotor shaft.

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Centrifuge 5430/ 5430 R - Fast, versatile, compact

High-speed centrifuges Centrifuge 5430 and 5430 R

Eppendorf Centrifuge 5430 R with 12 centrifuge rotors

Rotary knob control on the Centrifuge 5430

Centrifuge rotor for microplates on Centrifuge 5430

Eppendorf Rotor A-2-MTP for Centrifuge 5430 with lid

Eppendorf centrifuge Rotor F-35-6-30 with 50 mL Conical tubes

Eppendorf centrifuge Rotor F-45-48-11 for Centrifuge 5430 R with lid

Eppendorf centrifuge Rotor FA-45-30-11 for Centrifuge 5430 with lid

Eppendorf Rotor FA-45-24-11-Kit for Centrifuge 5430 with lid

Eppendorf Rotor F-45-64-5-PCR for Centrifuge 5430 with lid

Large backlit display on high-speed centrifuge Centrifuge 5430 R

Eppendorf Rotor F-45-18-17-Cryo for Centrifuge 5430 with lid and cryovial adapters

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Combine the best of both worlds with Centrifuge 5430 and 5430 R. These high-speed centrifuges have the small footprint of a microcentrifuge and the versatility of a multipurpose centrifuge.

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1 Single Devices

Centrifuge 5430 R

keypad, refrigerated, without rotor
230 V/50 – 60 Hz (CN)

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Catalog no. 5428000290

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Product Information

Technical Data

Features

The 5430 family with the Centrifuge 5430 and 5430 R combines the best features of a microcentrifuge (small footprint) and multipurpose centrifuge (versatility) in one instrument. The high-speed centrifuges offer centrifuge rotors for Eppendorf tubes and PCR strips, but also rotors for microplates and 15 or 50 mL conical tubes.
Additional Eppendorf QuickLock^® centrifuge rotors are now available in the rotor program, including 48-place rotors (1.5 mL or 2.0 mL), a 16-place rotor for Eppendorf Tubes 5.0 mL and a swing-bucket rotor (24 x 1.5 mL or 2.0 mL). With their unmatched versatility, Centrifuge 5430 and Centrifuge 5430 R are the flagship products among the Eppendorf microcentrifuge portfolio.
Available as keypad or knob variant.

Simple and safe handling

Aerosol-tight Eppendorf QuickLock rotors close with just one quarter of a turn. This not only saves you time during everyday use; it also takes strain off your wrist.

The Eppendorf Centrifuge 5430 R: Compact footprint and low access height

The compressor at the back of the Centrifuge 5430 R, the refrigerated version of Centrifuge 5430, is compact and has a low access height.

Advanced operating system of Centrifuge 5430 and 5430 R

The Centrifuge 5430 and 5430 R comes with an advanced operating system, making them more energy efficient due to the smart ECO shut-off and FastTemp pre-cooling functions. They each have a menu-driven, multilingual operation menu with a large backlit display that allows you to save up to 50 user-defined programs and five program keys for easy access to routine programs.
In addition to the standard FastTemp pre-cooling program, Eppendorf high-speed centrifuge Centrifuge 5430 R features the unique software option called FastTemp pro^®. The FastTemp pro function is available for fast automated pre-cooling based on pre-programmable times and dates. FastTemp pro can be set to a specific date or as a repetitive event on specified days of the week. Simply turn the centrifuge into standby mode when you leave your lab and let FastTemp pro take care of pre-cooling in the morning. This not only makes operation easy; it also saves you up to 90 % of energy overnight.
The ECO shut-off function saves you up to 88 % energy overnight by deactivating the compressor after 8 hours of inactivity to extend compressor life. This function can be deactivated in the menu settings.

Proud owner of an Eppendorf product? Already registered?

Did you know that if you register your Eppendorf Centrifuge 5430 with us you will get the following?
• 100 epPoints^® (for each product you register) to redeem in our rewards shop
• Additional 3 months warranty when you register a centrifuge, shaker, CO₂ incubator or cycler
• Entry in an exclusive Eppendorf prize draw (for each product you register)
• Get your personal product story to be posted on the Eppendorf website

Getting started is super easy! Either -
1. Download the Eppendorf App, scan and register OR
2. Visit our webpage, log onto your myEppendorf account and enter the product serial number
More information: www.eppendorf.link/product-registration

End-of-life

Disposal of packaging
The packaging of the Eppendorf Centrifuge 5430 contains different materials, this can include a wooden pallet, cardboard, a plastic dust cover, and some foam parts. Recycling of materials becomes more and more important every day: Are you aware that nearly all cardboard material is recycled in Europe? Our centrifuge packaging cardboard material consists of ca. 50% recycling material. Please support the global sustainability initiative of recycling valuable raw material by also collecting the freezer cardboard packaging material and disposing of it in the appropriate collection container at your organization. In respect to the plastic dust cover made of LD-PE and the foam parts, we recommend to select a dedicated recycling partner where PE material can be recycled. We suggest you contact your local waste hauler or facility management team to understand the available recycling options for your organization.

Disposal of instrument
Our Centrifuge 5430 and 5430 R last for many years, but if they need to be replaced, we kindly ask you to fulfill local requirements for disposal of these instruments. We strongly recommend a certified local recycling partner with experience in instruments with active cooling. Keeping it “local“ reduces the impact of transportation, and the "certified" aspect is recommended due to the safe and sustainable removal and recycling of the cooling liquids if it is a refrigerated centrifuge.

Decontamination
This piece of equipment was used in a laboratory and/ or was used to handle biological samples. Please keep in mind to adequately decontaminate the equipment which needs to be disposed. Check local requirements. For more information, get in contact with your local biosafety officer and/ or waste officer. Check if your local recycling partner has special instructions and/ or documentation requirements. You may also use the Eppendorf decontamination form sheet as guidance.

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Specifications	Centrifuge 5430 R

Max. RCF	−
Max. RCF with fixed-angle rotor	−
Max. RCF with swing-bucket rotor	−
Speed	100 – 17,500 rpm (100 rpm steps)
Product type	Centrifuges
Rotors available	−
Max. capacity	48 × 1.5/2.0 mL, 6 × 50 mL, 2 × MTP
Acceleration time¹⁾	−
Deceleration time¹⁾	−
Timer	30 s to 99:59 h, with continuous run function
SOFT brake function	yes
Noise level	< 54 dB(A) with Rotor F-45-30-11
Power supply	230 V, 50 – 60 Hz
Max. power consumption	−
Dimensions (W × D × H)	38 × 64 × 29 cm / 15 × 25.2 × 11.7 in
Product weight	56 kg / 123.5 lb
Height (with open lid)	63 cm / 24.8 in
Cooling	refrigerated
Temperature range	-11 °C to +40 °C

1)Acceleration and deceleration times may vary depending on rotor, load and voltage version.

Downloads: Centrifuge 5430/ 5430 R

Selection Guide

Centrifuge Family Overview

Overview of all Centrifuges: Capacity and Technical Data

Eppendorf Microcentrifuges (GLP)

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English

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Centrifuge 5430/ 5430 R - Fast, versatile, compact

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Product Information

Simple and safe handling

The Eppendorf Centrifuge 5430 R: Compact footprint and low access height

Advanced operating system of Centrifuge 5430 and 5430 R

Proud owner of an Eppendorf product? Already registered?

End-of-life

Technical Data

Features

Special features for Centrifuge 5430 R

Downloads: Centrifuge 5430/ 5430 R

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Centrifuge 5430/ 5430 R - Fast, versatile, compact

High-speed centrifuges Centrifuge 5430 and 5430 R

Eppendorf Centrifuge 5430 R with 12 centrifuge rotors

Rotary knob control on the Centrifuge 5430

Keypad control on the Centrifuge 5430

Centrifuge rotor for microplates on Centrifuge 5430

Eppendorf Rotor A-2-MTP for Centrifuge 5430 with lid

Eppendorf centrifuge Rotor F-35-6-30 with 50 mL Conical tubes

Eppendorf centrifuge Rotor F-45-48-11 for Centrifuge 5430 R with lid

Eppendorf centrifuge Rotor FA-45-30-11 for Centrifuge 5430 with lid

Eppendorf Rotor F-45-30-11 with lid

Eppendorf Rotor FA-45-24-11-Kit for Centrifuge 5430 with lid

Eppendorf Rotor F-45-64-5-PCR for Centrifuge 5430 with lid

Large backlit display on high-speed centrifuge Centrifuge 5430 R

Eppendorf Rotor F-45-18-17-Cryo for Centrifuge 5430 with lid and cryovial adapters

Do I need IVD?

Centrifuge-5430-R

Product Information

Simple and safe handling

The Eppendorf Centrifuge 5430 R: Compact footprint and low access height

Advanced operating system of Centrifuge 5430 and 5430 R

Proud owner of an Eppendorf product? Already registered?

End-of-life

Technical Data

Features

Special features for Centrifuge 5430 R

Downloads: Centrifuge 5430/ 5430 R