I have noticed that more solution is used for reverse pipetting than for normal pipetting. What is the reason for this?

Reverse pipetting means uptake of liquid including the blow-out volume and dispensing of liquid without the blow-out volume. Reverse pipetting is recommended to reduce the error with viscous or foaming liquids or when dispensing very small sample volumes. The excess solution aspirated remains in the tip and is discarded.

Can you recommend any literature on reverse pipetting?

Userguides 20 and 21 describe the technique and give application examples. They can be downloaded from the Knowledge Base on our website.

Should the pipette tip be wetted with liquid before pipetting volumes smaller than 10 µL?

Regardless of the volume, the air cushion should be wetted by multiple filling and emptying of the tip before dispensing.

What is the best way to pipette viscous liquids?

When pipetting viscous liquids, we recommend positive displacement instruments like Biomaster, Multipette/Repeater, and Varipette or an air cushion pipette (Research plus, Reference 2) in combination with the reverse pipetting technique (see Userguide 20 and 21 for details).

Is there an Eppendorf pipette which can be used to pipette 40 % polyethylene glycol 8000 (PEG)?

Yes, for pipetting PEG instruments operating according to the positive displacement principle like the Biomaster or a Multipette/Repeater can be used.

What is the best way to pipette chloroform?

When pipetting chloroform, we recommend positive displacement instruments like Biomaster, Multipette/Repeater, and Varipette or an air cushion pipette (Research plus, Reference 2) in combination with the reverse pipetting technique (see Userguide 20 and 21 for details).

What is the best way to pipette diethyl ether?

Diethyl ether has such a high vapor pressure that it would leak out of the tip immediately when an air cushion pipette is used. For this reason, it can only be pipetted using a positive displacement pipette, such as Biomaster, Multipette/Repeater, and Varipette.

Are Eppendorf pipettes resistant to acetonitrile?

Yes, Eppendorf pipettes are resistant to acetonitrile but care must be taken to avoid damage to printing and display window.

What should be considered when pipetting methanol with air cushion pipettes?

Due to the increased vapor pressure, we recommend that the tip is prewetted at least 3 times and that the reverse pipetting technique is used.

What is the best pipette for pipetting a 99% solution of n-amyl alcohol?

A 99 % solution of n-amyl alcohol is a highly viscous solution. We recommend working with an instrument that operates according to the positive displacement principle like Multipette/Repeater, Varipette, or Biomaster.

What is the best way to dispense organic solvent?

It is best to use a positive displacement device like the Multipette/Repeater. Positive displacement devices are most suitable for precise and drip-free dispensing of liquids with high vapor pressure. As the liquid is enclosed in a tip with integrated piston, the solvent vapor does not come into direct contact with the instrument.

I would like to pipette dichloromethane and n-hexanol very precisely. Can I use air cushion pipettes for this application?

Both dichloromethane and n-hexanol have a very high vapor pressure. For that reason, positive displacement instruments like the Biomaster and the Multipette/Repeater should be used.

Are your pipettes free of nucleic acids?

No, we cannot guarantee this. Of course we work carefully and cleanly in production, but no special measures are taken to prevent the infiltration of nucleic acids. To protect your pipette from contamination, we recommend using positive displacement pipettes, for example, our Biomaster or filtertips (ep Dualfilter T.I.P.S. or ep Dualfilter T.I.P.S. SealMax).

How can radioactive contamination be removed from Eppendorf pipettes?

Following contamination of pipettes with radioactive liquids, we recommend placing the contaminated parts in a complex-forming liquid or special cleaning solution. Then rinse very thoroughly with distilled water and dry. Grease piston lightly afterwards.

Do Eppendorf pipettes always need to be greased?

No, the piston only has to be greased if grease-dissolving solutions like organic solvents have been used.

Can the pipette withstand evacuation (applying a vacuum)?

No, air cushion pipettes should not be used under vacuum, as the pressure ratios are not right.

How should warm or cold liquids be pipetted?

Exactly in the same way as liquids at room temperature, however, please note that solutions may behave differently. In addition, the pipetting accuracy / precision may be outside the permissible error limits. In order to achieve maximum accuracy / precision, all components should have the same temperature: pipette, tips, liquid, and the surrounding air.

Are the handles of Eppendorf pipettes made of a special material to minimize temperature effects during pipetting?

Temperature effects are minimized in our pipettes by their design. We use a special polypropylene which satisfies the special requirements on a pipette handle.

Do Eppendorf pipettes always have to be kept in a stand or can they also be put down on the laboratory bench?

They can be put down on the laboratory bench - but never with a full tip.

I often work with different chemicals. Can you tell me when I need to adjust my pipettes?

This belongs to the permissible errors of your specific application. In general an adjustment of air cushion pipettes, in other words altering the stroke of the pipette, is required for solutions of a density higher 1.2 mg/µL. Please be aware that variable manual pipettes turn into "fixed-volume pipettes" when being adjusted: They can only be adjusted to a specific liquid to one volume and not across the entire dispensing range. Eppendorf manual pipettes have a user adjustment for temporary adjustment purposes. This user adjustment can easily be set back to manufacturer settings without the need for recalibration. For the correct procedure and examples for settings of user adjustments for different liquids please see the operating instructions and CD enclosed to your pipette.

How do I determine the volume values when calibrating gravimetrically?

The measuring values of the analysis scales are multiplied by the correction factor Z (see SOP pipettes). This takes into account the density of the water at the relevant temperature and the relevant air.

What does EN ISO 8655 cover?

ISO 8655 addresses the needs of suppliers, test houses, and users and specifies the general requirements for piston-operated liquid handling devices. It is structured in seven chapters: ISO 8655-1: Terminology, general requirements, and user recommendations ISO 8655-2: Piston pipettes ISO 8655-3: Piston burettes ISO 8655-4: Dilutors ISO 8655-5: Dispensers ISO 8655-6: Gravimetric method for the determination of measurement error ISO 8655-7: Non-gravimetric methods for assessment of equipment performance (titration, photometry)

What do the terms random error and systematic error mean in a pipette calibration?

The systematic error describes the deviation of the dispensed volume from the nominal volume or from the selected volume of the piston stroke device, and is determined by establishing the mean value of (usually) 10 measurements. The random error describes the mean variation of the dispensed volumes from the mean value of the dispensed volumes, and is determined by calculating the repeat standard deviation of (usually) 10 measurements.

Why are the Z factors listed in our SOP different from factors which can be derived from density reference values (of water)?

The Z factor is included in EN ISO 8655-6 and was incorporated into our SOP. As we understand it, it was incorporated in the standard by the Physikalisch Technische Bundesanstalt in Braunschweig. The Z factor describes the density of water at a particular temperature using a 5th degree polynomial. Furthermore, the density of water is corrected by the density of air (derived from air pressure) and a fixed correction value for balances. These corrections are the key reasons for the deviation from density values in various books of tables.

At which volumes should a variable pipette be calibrated?

According to the EN ISO 8655 standard, a variable pipette should be calibrated to 3 volumes during conformity testing: 1. Nominal volume, 2. 50 % of nominal volume, 3. Smallest value that can be set or 10 % of nominal volume.

What is the nominal volume of a variable pipette?

The nominal volume of a variable pipette is always the maximum volume. For a 100 - 1,000 µL pipette, it is therefore 1,000 µL.

I very frequently work with different chemicals. Can I still perform pipette calibration using water?

Calibration, i.e. checking a pipette on a balance, is always performed using distilled or deionized water (see EN ISO 8655).

During weighing (pipette calibration) I obtained steadily decreasing measuring results. Why is this?

The weight fluctuations you measured would appear to be a temperature problem. Please ensure that the pipette, the distilled water used for testing, the tips etc. are all at the same temperature. It is recommended that the pipettes be placed in the weighing room at least 2 hours before the test is performed. While measuring, ensure that transfer of heat from your hands is kept to a minimum (otherwise weight fluctuations may occur!) Tip: do not constantly grasp the pipette in the palm of your hand while waiting for each measurement result! We recommend our standard operating procedure (SOP) for pipettes as a source of basic information on pipette calibration.

How are multi-channel pipettes tested?

According to ISO 8655, all channels of multi-channel pipettes are to be tested. All channels have to be filled but only the channel to be tested is measured. Because of the high number of measurements (8-channel pipette: 240 measurements, 12-channel pipette: 360 measurements), it makes sense to send the multi-channel pipette to a professional calibration lab. These labs have special constructions for balances to ensure that all channels are filled but only one channel is measured.

Is the pipette grease free of resins?

Yes, the pipette grease supplied by Eppendorf is free of resins.

How is the wall mount for pipettes attached to the wall?

The pipette holder is affixed to the wall with adhesive.

Can the Xplorer be autoclaved, and how often?

The lower part of the Xplorer can be autoclaved. 175 autoclaving cycles (121 °C, 1 bar, 20 min.) were tested. This number of tests corresponds to the average use over 5 years.

Is the Xplorer resistant to solvents?

Yes, the Xplorer is resistant to solvents. Only the display shall not come into contact with the solvent.

Can the Xplorer be readjusted?

Yes, there are several options for adjusting the pipette in the ""Options"" operating mode, e.g. to 75 % ethanol, altitude, elongated tips. Furthermore, individual adjustments are possible, as 1-point, 2-point or 3-point settings.

Which volumes are used for mixing in the mode "Pipetting and mixing"?

In the "Pipetting and mixing" operating mode, the mixing volume can be defined independently from the filling volume.

Can the acoustic feedback of the Xplorer be turned off?

Yes, the volume of the acoustic feedback can be set to levels 0-8 in the "Options" operating mode.

How can accidental parameter changes be prevented?

A complete run with volume, speed, etc. can be saved at the program level. After exiting the program level, the run can no longer be changed just by pressing the keys. Additionally, the Xplorer plus offers possibilities of protecting settings by password.

Various speeds can be selected with the Xplorer. What speeds do the settings 1-8 represent?

This is depending on the aspiration volume. Please see operating manual in case of further questions.

How long does it take the Xplorer to aspirate 10 ml?

Depending on the speed level, the Xplorer pipette needs for aspiration of 10 mL between 12 s (Step 1) and 2.6 s (Step 8).

Are the Xplorer and Xplorer plus resistant against UV-light?

The Xplorer is UV-resistant. Longer periods of irradiation can lead to very minimal material discoloration that does not have any effect on functionality.

Does the technical data also apply to the "Dispensing" operating mode?

The Xplorer technical data is only valid for pipetting. Conformity must be achieved for this operating mode. For piston stroke pipettes, there are no requirements for dispensing accuracy according to EN ISO 8655.

Can the aspiration volume be limited?

Yes, a volume limit can be defined in the "Options" operating mode.

What charging possibilities are available for the Xplorer?

The Xplorer can be charged using the power supply included in the accessories or in the charging stand or charging carousel (optional accessories). One Xplorer can be charged in the charging stand and four in the charging carousel. A single-channel or multi-channel Xplorer can be attached to a charging shell.

Can the Xplorer also be charged in Multipette charging stands?

No, the Xplorer cannot be charged in the Multipette charging stand. But the charging shells of the Xplorer charging stand can be exchanged. A charging shell for a Multipette stream/Xstream can be used instead of a charging shell for an Xplorer.

How long is the service life of the battery?

The lithium polymer battery features high energy density. Its service life is several years; but this is strongly dependent on usage and storage conditions.

Can the battery be reordered?

Yes, batteries for electronic liquid handling can be ordered separately as spare parts and should be replaced after approx. 3 years of use.

Which pipette tips fit on the Xplorer?

The certificate of conformity for the Eppendorf Xplorer ® system is valid for epTIPS. However, the Xplorer can also be used with common competitor tips.

Is it possible to mix liquids in the micro test plate or small volumes with the preparation?

In the operating mode "Pipetting and Mixing," the sample is automatically mixed with the preparation after dispensing. The mixing cycles and mixing volume can be individually defined for this.

Can I precisely dispense liquids such as ethanol or glycerol with the Xplorer?

On the Xplorer, the pipette can be adjusted to different liquids or geographic heights using 1, 2 or 3-point adjustment, taking into account the corresponding density and associated scale results. The setting for ethanol 75 %25 or glycerol 50 %25 is completed using an internally determined factor that takes into account the density.

When pipetting foaming liquids (e.g. cell culture media) I‘m transferring foam which got trapped between the epT.I.PS. 384. How can I improve?

When pipetting foaming liquids it may happen that foam adhere between the tips. Make sure to pipette slowly avoiding foam formation. Importantly, do not immerse the tip too deeply into the liquid preventing foam getting caught between the tips.

How often should pipettes be calibrated?

The user decides upon the frequency of calibration, the number of test volumes and the number of measurements for each test volume within the framework of monitoring his test materials. This can vary depending on the frequency of use of the pipette, the number of users of the device, the aggressiveness of the liquids to be dispensed and the error limits deemed acceptable by the user. However, an inspection should be carried out at least once a year. See also EN ISO 8655-6.

text.skipToContent text.skipToNavigation

CZ | CZK

Select your settings

* = required

Select your country/region *

(China) Hong Kong
(China) Taiwan
Afghanistan
Albania
Algeria
Angola
Anguilla
Antigua and Barbuda
Argentina
Armenia
Aruba
Australia
Austria
Azerbaijan
Bahamas
Bahrain
Bangladesh
Barbados
Belgium
Belize
Benin
Bermuda
Bhutan
Bolivia
Bosnia and Herzegovina
Botswana
Brazil
Brunei Darussalam
Bulgaria
Burkina Faso
Burundi
Cambodia
Cameroon
Canada
Cape Verde
Cayman Islands
Central African Republic
Chad
Chile
China
Christmas Island
Cocos (Keeling) Islands
Colombia
Comoros
Congo
Congo, the Democratic Republic of the
Cook Islands
Costa Rica
Cote d'Ivoire
Croatia
Curaçao
Cyprus
Czech Republic
Denmark
Djibouti
Dominica
Dominican Republic
Ecuador
Egypt
El Salvador
Equatorial Guinea
Eritrea
Estonia
Ethiopia
Falkland Islands
Faroe Islands
Fiji
Finland
France
French Guiana
French Polynesia
Gabon
Gambia
Georgia
Germany
Ghana
Gibraltar
Greece
Greenland
Grenada
Guadeloupe
Guatemala
Guernsey
Guinea
Guinea-Bissau
Guyana
Haiti
Honduras
Hungary
Iceland
India
Indonesia
Iraq
Ireland
Isle of Man
Israel
Italy
Jamaica
Japan
Jersey
Jordan
Kazakhstan
Kenya
Kiribati
Korea, Republic of
Kosovo
Kuwait
Kyrgyzstan
Lao People's Democratic Republic
Latvia
Lebanon
Lesotho
Liberia
Libyan Arab Jamahiriya
Liechtenstein
Lithuania
Luxembourg
Macedonia
Madagascar
Malawi
Malaysia
Maldives
Mali
Malta
Marshall Islands
Martinique
Mauritania
Mauritius
Mayotte
Mexico
Micronesia, Federated States of
Moldova
Monaco
Mongolia
Montenegro
Montserrat
Morocco
Mozambique
Myanmar
Namibia
Nauru
Nepal
Netherlands
New Caledonia
New Zealand
Nicaragua
Niger
Nigeria
Niue
Norfolk Island
Norway
Oman
Pakistan
Palau
Palestinian Territory
Panama
Papua New Guinea
Paraguay
Peru
Philippines
Pitcairn
Poland
Portugal
Qatar
Reunion
Romania
Rwanda
Saint Barthélemy
Saint Kitts and Nevis
Saint Lucia
Saint Martin (French part)
Saint Vincent and the Grenadines
Samoa
San Marino
Sao Tome and Principe
Saudi Arabia
Senegal
Serbia
Seychelles
Sierra Leone
Singapore
Sint Maarten (Dutch part)
Slovakia
Slovenia
Solomon Islands
Somalia
South Africa
Spain
Sri Lanka
Suriname
Swaziland
Sweden
Switzerland
Tajikistan
Tanzania
Thailand
Timor-Leste
Togo
Tokelau
Tonga
Trinidad and Tobago
Tunisia
Turkey
Turkmenistan
Turks and Caicos Islands
Tuvalu
Uganda
Ukraine
United Arab Emirates
United Kingdom
United States
Uruguay
Uzbekistan
Vanuatu
Venezuela
Viet Nam
Virgin Islands, British
Wallis and Futuna
Western Sahara
Yemen
Zambia
Zimbabwe

Select your language *

English

Select your currency *

Please be aware that your shopping cart cannot be restored on the newly changed country website or when you come back. If you want to save your cart, please login.

myEppendorf

Quick Order

Quote

Service & Support
Lab Academy
Beyond Science
Company & Careers

Service & Support
Lab Academy
Beyond Science
Company & Careers

CZ | CZK

Select your settings

* = required

Select your country/region *

(China) Hong Kong
(China) Taiwan
Afghanistan
Albania
Algeria
Angola
Anguilla
Antigua and Barbuda
Argentina
Armenia
Aruba
Australia
Austria
Azerbaijan
Bahamas
Bahrain
Bangladesh
Barbados
Belgium
Belize
Benin
Bermuda
Bhutan
Bolivia
Bosnia and Herzegovina
Botswana
Brazil
Brunei Darussalam
Bulgaria
Burkina Faso
Burundi
Cambodia
Cameroon
Canada
Cape Verde
Cayman Islands
Central African Republic
Chad
Chile
China
Christmas Island
Cocos (Keeling) Islands
Colombia
Comoros
Congo
Congo, the Democratic Republic of the
Cook Islands
Costa Rica
Cote d'Ivoire
Croatia
Curaçao
Cyprus
Czech Republic
Denmark
Djibouti
Dominica
Dominican Republic
Ecuador
Egypt
El Salvador
Equatorial Guinea
Eritrea
Estonia
Ethiopia
Falkland Islands
Faroe Islands
Fiji
Finland
France
French Guiana
French Polynesia
Gabon
Gambia
Georgia
Germany
Ghana
Gibraltar
Greece
Greenland
Grenada
Guadeloupe
Guatemala
Guernsey
Guinea
Guinea-Bissau
Guyana
Haiti
Honduras
Hungary
Iceland
India
Indonesia
Iraq
Ireland
Isle of Man
Israel
Italy
Jamaica
Japan
Jersey
Jordan
Kazakhstan
Kenya
Kiribati
Korea, Republic of
Kosovo
Kuwait
Kyrgyzstan
Lao People's Democratic Republic
Latvia
Lebanon
Lesotho
Liberia
Libyan Arab Jamahiriya
Liechtenstein
Lithuania
Luxembourg
Macedonia
Madagascar
Malawi
Malaysia
Maldives
Mali
Malta
Marshall Islands
Martinique
Mauritania
Mauritius
Mayotte
Mexico
Micronesia, Federated States of
Moldova
Monaco
Mongolia
Montenegro
Montserrat
Morocco
Mozambique
Myanmar
Namibia
Nauru
Nepal
Netherlands
New Caledonia
New Zealand
Nicaragua
Niger
Nigeria
Niue
Norfolk Island
Norway
Oman
Pakistan
Palau
Palestinian Territory
Panama
Papua New Guinea
Paraguay
Peru
Philippines
Pitcairn
Poland
Portugal
Qatar
Reunion
Romania
Rwanda
Saint Barthélemy
Saint Kitts and Nevis
Saint Lucia
Saint Martin (French part)
Saint Vincent and the Grenadines
Samoa
San Marino
Sao Tome and Principe
Saudi Arabia
Senegal
Serbia
Seychelles
Sierra Leone
Singapore
Sint Maarten (Dutch part)
Slovakia
Slovenia
Solomon Islands
Somalia
South Africa
Spain
Sri Lanka
Suriname
Swaziland
Sweden
Switzerland
Tajikistan
Tanzania
Thailand
Timor-Leste
Togo
Tokelau
Tonga
Trinidad and Tobago
Tunisia
Turkey
Turkmenistan
Turks and Caicos Islands
Tuvalu
Uganda
Ukraine
United Arab Emirates
United Kingdom
United States
Uruguay
Uzbekistan
Vanuatu
Venezuela
Viet Nam
Virgin Islands, British
Wallis and Futuna
Western Sahara
Yemen
Zambia
Zimbabwe

Select your language *

English

Select your currency *

Please be aware that your shopping cart cannot be restored on the newly changed country website or when you come back. If you want to save your cart, please login.

Automated Pipetting
Automated Pipetting
Bioprocess
Bioprocess
Cell Incubation
Cell Incubation
- CO2 Incubators
- Sample and Information Management
Centrifugation
Centrifugation
Cold Storage
Cold Storage
Digital Lab Management
Digital Lab Management
- Sample and Information Management
Laboratory Consumables
Laboratory Consumables
Lab Shakers
Lab Shakers
Liquid Handling
Liquid Handling
PCR
PCR
Photometry
Photometry
Services
Services
Temperature Control and Mixing
Temperature Control and Mixing
- Accessories
- Instruments
Promotions
OEM

myEppendorf

epPoints®
Product Registration
- Register Products
Order History
Redeem Quote
Lists
- Batch Order
My Profile
Deactivate Account

No results found

Search Suggestions

Product Families

Show all Product Families ()

Article

Show all Articles ()

Content

Show all Content ()

Home
Products
Liquid Handling
Manual Pipettes & Dispensers
Eppendorf Xplorer^®/ Eppendorf Xplorer^® plus

Eppendorf Xplorer^®/ Eppendorf Xplorer^® plus - Electronic Pipette

Eppendorf Xplorer® / Xplorer plus single- and multi-channel electronic pipettes

Eppendorf Xplorer_REG_ plus electronic single-channel pipette

Eppendorf Xplorer_REG_ plus electronic pipette display and multi-function rocker

Eppendorf Xplorer_REG_ (plus) multi-channel electronic pipettes speed up your applications with microplates

Eppendorf Xplorer_REG_ plus 16 and 24-channel electronic pipettes are ideal to fill 384-well plates

Fill 384-well plates with ease using Eppendorf multi-channel pipettes with 16 or 24 channels

The Pipette Carousel 2 with Eppendorf Xplorer_REG_ electronic pipettes

Discover the smooth handling of Eppendorf Xplorer_REG_ pipettes yourself

Female scientist using Eppendorf Xplorer_REG_ plus electronic pipette to fill an Eppendorf Tube_REG_

Man using Eppendorf Xplorer_REG_ plus 24-channel pipette to fill a 384-well plate

Eppendorf Xplorer_REG_ electronic single-channel pipette used to fill an Eppendorf Tube_REG_ in the lab

The software of your Eppendorf Xplorer_REG_ plus pipette can easily be updated using the Pipette Software Update Tool

ACT label of sustainability certification for Eppendorf Xplorer_REG_ plus pipette, 100-1000 _MICRO_L (blue), single-channel

Videos not loading, because cookies have been rejected. Change your

Buy 5 boxes of epT.I.P.S. BioBased, save 50% on an Xplorer single-channel pipette, buy 10 boxes and save 50% on an Xplorer multi-channel pipette - Promotion Code: WhatsNext2024

Promotion
New

Product Information

People who give 100 % every day deserve the best tools and the best laboratory equipment. The Eppendorf Xplorer and Eppendorf Xplorer plus electronic pipettes are single-, 8-, 12-, 16- and 24-channel pipettes designed to aspirate and dispense liquid volumes from 0.5 µL to 10 mL, while eliminating many of the potential error risks associated with manual pipetting. With the Eppendorf Xplorer/Xplorer plus electronic pipette, your work achieves a new level of simplicity, precision and reproducibility.

Show more Product Information

Discover the Pipette Manager to extend the features of your Xplorer (plus) pipette even further!

Easily update the firmware of your electronic pipette with our Pipette Software Update Tool
Learn more about sustainability & ergonomics at Eppendorf
Interested in the Move It^® adjustable tip spacing variants of this pipette model?