Pipetting of individual volumes sequentially like serial dilution of nucleic acids, cells, proteins, antibiotics, and other substances like dyes, buffers or reagents belong to the routine applications in the lab. The application spectrum is widespread ranging from preparation of standard curves, photometric DNA quantification, or Bradford protein quantification to the creation of sample stocks.
Taking a closer look at the serial dilution technique, one quickly concludes that it is repetitive and time-consuming. In the worst case, the mechanical pipette has to be adjusted to the desired volumes after each step - and several times in a row. In short, there are four hassles:
- Double work: A series of only 5 concentrations requires readjusting the volume 10 times.
- Many arm movements due to repeated liquid aspiration and dispensing – from reagent to tube, and vice versa.
- Repetitive refocusing to continue at the right row/column for liquid dispensing.
- Impairment of precision due to the normal irregularities like user-dependent thumb speed or varying operation forces. Being essential for dilution series, precision is dependent on pipetting uniformly over each step.
Up to 10 individual volumes in one go
If you want to save time and prefer not to work at the expense of precision when performing serial dilutions
, sequential dispensing with an electronic pipette is a solution. Sequential dispensing means to aspirate once and dispense up to 10 user-defined volumes in sequential order in one go.
This means, up to 10 times less work compared to mechanic pipetting!
How it works:1. Adjust
Choose the number of pipetting steps and the respective volumes in the sequential dispensing mode2. Pipette liquid #1
Aspirate the entire volume of liquid #1 only once.
Dispense the preset individual volumes in several vessels sequentially one after the other without aspirating again.3. Pipette liquid #2
Aspirate the entire volume of liquid #2 only once.
Dispense in opposite direction (reverse vessel order) sequentially without aspirating again.