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NGS methods for epMotion® 5073m NGS and 5075t NGS

The epMotion® can be equipped with an on-deck thermal mixer and is proven to be the most efficient configuration at enabling automated NGS library preparation. For such preconfigured solutions, a list of NGS library preparation workflows have been automated according to the manufacturer’s user guides. An exemplary, non-limiting selection, of kits, have been established below. Your local specialist would be happy to further discuss your specific needs and further kit options:

epMotion® 5073m NGS solution

Illumina® 16S Metagenomic
AmpliSeq® for Illumina
KAPA Library Quant Kit (LQK)

epMotion® 5057t NGS solution

Ampliseq for Illumina® : Cancer HotSpot Panel v2
Ampliseq for Illumina® : Focus Panel
Ampliseq for Illumina® : Comprehensive Panel Panel v3
Illumina® TruSeq® Stranded Total RNA (RiboZero)
Illumina® TruSeq® Stranded mRNA
Illumina® TruSeq® Nano DNA
Illumina® TruSeq® DNA PCR-Free
Illumina® TruSeq® Rapid Exome
Illumina® TruSeq® RNA Exome
Illumina® TruSeq® Custom Amplicon Low Input
Illumina® TruSight® HLA v2
Illumina® TruSight® Tumor 15
Illumina® TruSight® RNA Pan Cancer
Illumina® TruSight® RNA Fusion
Illumina® TruSight® Cancer/TruSight® Enrichment
Illumina® TruSight® Rapid Capture
Illumina® TruSight® One
Illumina® Nextera® XT DNA
Illumina® Nextera® Rapid Capture Custom Enrichment
Illumina® Nextera® DNA Flex
Illumina® Nextera® Flex for Enrichment
KAPA® HyperPrep
KAPA® mRNA HyperPrep
KAPA® HyperPlus
KAPA® Stranded RNA Seq
KAPA® Library Quant
The following kits have been integrated successfully in customers' workflows with the assistance of our automation experts. Many were modified and optimized to work with the individual situation, such as the epMotion® 5075 NGS configuration, available accessories and consumable choices:

Sucessfully integrated kits

Illumina® Nextera® Rapid Capture Exome
Illumina® ForenSeq™
KAPA® RNA HyperPrep
KAPA® Ribo Erase
Agilent® HaloPlex®
Agilent® SureSelect® XT
New England Biolabs® NEBNext® Ultra
New England Biolabs® NEBNext® Ultra II
Qiagen® QIAseq FX
Qiagen® GeneRead™ Panel


Illumina® is a leading provider of next-generation sequencing technology used for the analysis of genetic variation and function. Our portfolio of sequencing systems offers a range of options for diverse study designs—from large-scale analyses involving numerous whole genomes, to highly targeted analyses with smaller sample volumes. Illumina® systems are built on proven sequencing by synthesis (SBS) technology, which delivers incredibly accurate data and offers the most streamlined user workflow. To enable a seamless user experience, Illumina® also develops sample preparation kits and bioinformatic solutions that are optimized for the sequencing workflow. Our Nextera and TruSeq sample preparation technologies allow researchers to quickly and easily generate sequencing-ready libraries for virtually any genomic, transcriptomic, or epigenomic analysis.