Are Eppendorf Micro- and Multipurpose centrifuges equipped with an imbalance switch-off function?

Yes, Micro- and Multipurpose centrifuges are equipped with an imbalance detection. As soon as an imbalance is detectetd, an error message occurs on the display and the run is stopped automatically.

For which applications can I use Eppendorf Microcentrifuge 5420?

Eppendorf Microcentrifuge 5420 allows a broad variety of applications. Up to 24 micro test tubes can be centrifuged at 21,300 xg. It can be used with tubes from 0.2 up to 2.0 mL. Possible applications include: -Isolation of DNA, RNA and proteins - Spin columns / Filter inserts - Aerosol-tight centrifugation of infectious samples - Pelletizing of cells (bacteria, eukaryotic cells) up to 2.0 mL - Spin down of PCR mixtures in tubes and strips

What kind of tubes can be centrifuged in Eppendorf Microcentrifuge 5420?

Due to the 2 different rotors and adapters, a variety of tubes can be centrifuged with the Centrifuge 5420: • Micro test tubes (0.5 - 2.0 mL) • PCR tubes and strips • Spin columns / Filter inserts (1.5/2.0 mL) You will find a detailed list of usable tubes in the centrifuges operating manual (chapter technical data) which can be downloaded from the Eppendorf homepage.

What are the noise levels of Eppendorf Microcentrifuges?

The noise level of Eppendorf Microcentrifuges ranges from 49 dB(A) to 58 dB(A). What does that mean? Here are some examples of every day life noises: 45 db(A) - a quiet library or soft conversation at home 50 db(A) - the hum of a refrigerator 58 db(A) - dishwasher running in the next room Eppendorf Microcentrifuges have a very moderate noise level though. Please find the exact numbers in the section technical data on the respective homepages or in the respective Operating Manuals.

How can Eppendorf Micro- and Multipurpose centrifuges be opened in case of a power failure?

Each Eppendorf centrifuge is equipped with an emergency lid release. The centrifuge lid can be opened e.g. with the support of the rotor key. Detailed description of this topic is listed in the respective Operating Manual which can be downloaded from the Eppendorf homepage.

What is the minimum selectable rotational speed of an Eppendorf Microcentrifuge?

The minimum selectable speed is 100 rpm or 1 rcf (g-force).

Can the fixed-angle rotors of Eppendorf Microcentrifuges be operated without rotor lids?

The fixed-angle rotor FA-45-18-11 can be used with or without rotor lid. With rotor lid the centrifugation is aerosol-tight and the Centrifuge 5418/5418 R is even quieter. The aerosol-tight centrifugation can only be carried out with the rotor lid. Especially when working with pathogenic microorganisms, we recommend the usage of the aerosol-tight lid. When centrifuging filter devices like spin columns or other comparable tubes, the aerosol-tight rotor lid must be used in any case. This protects the centrifuge from damage – e. g. by torn-off tube lids.

What is the FastTemp function of refrigerated Eppendorf Micro- and Multipurpose centrifuges?

The FastTemp function is a specific temperature control run which was developed to reach the set temperature as quickly as possible. This temperature control run can already be started during sample preparation by pressing the FastTemp button. This enables a centrifugation at the desired temperature directly after sample preparation without time loss. Please find more detailed information of the FastTemp function in the respective operating manuals of Eppendorf Micro- and Multipurpose centrifuges on the Eppendorf homepage.

How can Eppendorf Micro- and Multipurpose centrifuges be cleaned?

For cleaning and disinfection of Eppendorf centrifuges you will find detailed information in the respective operating manuals which can be found in the section manuals on this page. Furthermore we recommend the following papers which can be found in the section Notes&Papers on the Eppendorf homepage. - Whitepaper 014 - Maintenance Poster - Logfile for Routine Maintenance

Can I use both - rpm and g-force (rcf) - to set the speed parameter of an Eppendorf Microcentrifuge?

Yes. Each Eppendorf Microcentrifuge has the possibility to use rpm or g-force (rcf) to set the speed. It is equipped with a special button to easily change between rpm and rcf. For the centrifuges Minispin / Minispin plus a combination of buttons have to be pressed. Please refer to the devices Operating Manuals for more detailed information. Information available on the product page on eppendorf.com.

What is the advantage of the automatic lid release of Eppendorf Microcentrifuges?

The automatic lid release is a feature of Eppendorf's air cooled Microcentrifuges. It can be switched On and Off via the centrifuges operating menu. If it is activated, the centrifuge lid opens automatically once the centrifugation run is finished. This allows quicker access to the samples and avoids possible heat build-up. In addition, the open lid is a clear signal - even from a distance - that the centrifugation run has ended. This function has not been implemented in the refrigerated microcentrifuges from Eppendorf, as a set temperature can be set there which should also be maintained after the end of a centrifuge run.

Does the Eppendorf Microcentrifuge 5420 have a possibility to protect programs against overwriting?

Yes, it has. Please find details of this function in the Operating Manual of the device.

What does the Finished Time function of Eppendorf Microcentrifuges mean?

The Finished Time function counts and shows the elapsed time since the end of the centrifuge run.

How does the Timer function of Eppendorf Microcentrifuges work?

The "Timer" function is able to delay the start of a centrifuge run. This can be used for many nucleic acid purification kits where the buffer on the column should be incubated before the sample is centrifuged. Please find detailed information in the respective devices manual on the Eppendorf homepage.

What is the Short Spin function of Eppendorf Micro- and Multipurpose centrifuges?

The Short Spin function was developed to be able to perform a quick centrifuge run without having to set parameters. To start a short spin centrifugation, simply press the short key. Centrifugation will continue for as long as it is pressed. Please find detailed information on this function in the respective devices manual which can be found on their Eppendorf hompages.

How should I lubricate the seal of the FA-24x2 rotor lid of Eppendorf Microcentrifuge 5420?

Please lubricate the FA-24x2 rotor lid as described in the Operating Manual or the rotor supplementary sheet. Information available at the product page on eppendorf.com.

How do I recognize aerosol-tight rotors?

Firstly, the aerosol-tight rotors can be recognized by their designation abbreviation “FA” or “AT”; secondly, Eppendorf has color-coded the aerosol-tight rotors. All aerosol-tight rotors have a red rotor lid screw, and a red ring is printed on to the rotor shaft.

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Centrifuge 5420 - Centrifuge for Eppendorf tubes

Centrifuge 5420 24x2 mL rotor, front view

Centrifuge 5420 24x2 rotor, side view, 2

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The ultimate centrifuge for Eppendorf tubes

The Eppendorf Centrifuge 5420 supports your spin column-based nucleic acid purification and gel-extraction workflows. This new centrifuge is a optimal centrifuge for Eppendorf tubes and offers 24-place capacity in an aerosol-tight rotor and speeds up to 21,300 × g in a small footprint.

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Centrifuge 5420

keypad, non-refrigerated, without rotor
230 V/50 – 60 Hz (EU)

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Catalog no. 5420000318

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14.200,00 DKK

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Versatile applications of Centrifuge 5420

The Centrifuge 5420 was designed with your molecular biology routine applications in mind. The spectrum of methods includes DNA/RNA purification, DNA extraction from agarose gels, and PCR reaction set-up. It has a particularly small footprint to save space on the lab bench and an easy-to-clean keypad control panel. You can spin up to 24 tubes from 1.5 to 2.0 mL. Optional adapters extend the volume range to 0.4 and 0.5 mL tubes as well as 0.6 mL BD Microtainer^®. A rotor for 32 PCR tubes (0.2 mL) or four PCR strips is available as well.

Safety and efficiency with Eppendorf Centrifuge 5420

For your safety, the standard rotor provides aerosol-tight containment for the centrifugation of hazardous samples such as viruses, pathogen bacteria or radioactive probes. This makes the centrifuge for Eppendorf tubes an excellent choice for various applications.

Proud owner of an Eppendorf product? Already registered?

Did you know that if you register your Eppendorf Centrifuge 5420 with us you will get the following?
• 100 epPoints^® (for each product you register) to redeem in our rewards shop
• Additional 3 months warranty when you register a centrifuge, shaker, CO₂ incubator or cycler
• Entry in an exclusive Eppendorf prize draw (for each product you register)
• Get your personal product story to be posted on the Eppendorf website

Getting started is super easy! Either -
1. Download the Eppendorf App, scan and register OR
2. Visit our webpage, log onto your myEppendorf account and enter the product serial number
More information: www.eppendorf.link/product-registration

End-of-life

Disposal of packaging
The packaging of the Eppendorf Centrifuge 5420 contains different materials, this can include a wooden pallet, cardboard, a plastic dust cover, and some foam parts. Recycling of materials becomes more and more important every day: Are you aware that nearly all cardboard material is recycled in Europe? Our centrifuge packaging cardboard material consists of ca. 50% recycling material. Please support the global sustainability initiative of recycling valuable raw material by also collecting the freezer cardboard packaging material and disposing of it in the appropriate collection container at your organization. In respect to the plastic dust cover made of LD-PE and the foam parts, we recommend to select a dedicated recycling partner where PE material can be recycled. We suggest you contact your local waste hauler or facility management team to understand the available recycling options for your organization.

Disposal of instrument
Our Eppendorf Centrifuge 5420 lasts for many years, but if it needs to be replaced, we kindly ask you to fulfill local requirements for disposal of these instruments. We strongly recommend a certified local recycling partner with experience in instruments with active cooling. Keeping it “local“ reduces the impact of transportation, and the "certified" aspect is recommended due to the safe and sustainable removal and recycling of the cooling liquids if it is a refrigerated centrifuge.

Decontamination
This piece of equipment was used in a laboratory and/ or was used to handle biological samples. Please keep in mind to adequately decontaminate the equipment which needs to be disposed. Check local requirements. For more information, get in contact with your local biosafety officer and/ or waste officer. Check if your local recycling partner has special instructions and/ or documentation requirements. You may also use the Eppendorf decontamination form sheet as guidance.

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Specifications	Centrifuge 5420

Max. RCF	21300 × g
Max. RCF with fixed-angle rotor	21300 × g
Speed	100 – 15,060 rpm
Product type	Centrifuges
Rotors available	2
Max. capacity	24 × 1.5/2.0 mL
Acceleration time¹⁾	15 s
Deceleration time¹⁾	15 s
Timer	10 s to 9:59 h, with continuous run function
SOFT brake function	10 ramps
Noise level	< 56 dB(A)
Power supply	230 V, 50 – 60 Hz
Max. power consumption	290 W
Dimensions (W × D × H)	24 × 35 × 22 cm / 9.4 × 13.8 × 8.7 in
Product weight	13 kg / 28.7 lb
Height (with open lid)	44 cm / 17.3 in
Cooling	non-refrigerated

1)Acceleration and deceleration times may vary depending on rotor, load and voltage version.

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Eppendorf Microcentrifuges (GLP)

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Selection Guide

Centrifuge Family Overview

Overview of all Centrifuges: Capacity and Technical Data

Centrifuge 5420 – designed with your molecular biology routine applications in mind

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Centrifuge 5420 - Centrifuge for Eppendorf tubes

Centrifuge 5420 side view, closed, 2

Centrifuge 5420 24x2 ml rotor, front view

Centrifuge 5420 24x2 rotor, side view, 2

Centrifuge 5420 display

Centrifuge 5420 front view, closed

Centrifuge 5420 closing 2

Centrifuge 5420 empty, side view, open

Centrifuge 5420 pcr rotor, hand

Rotor F-32x0.2-PCR pcr rotor, pcr tube strips

Rotor FA-24x2 24x2 rotor

Maintenance of Centrifuges

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The ultimate centrifuge for Eppendorf tubes

Product Information

Versatile applications of Centrifuge 5420

Safety and efficiency with Eppendorf Centrifuge 5420

Proud owner of an Eppendorf product? Already registered?

End-of-life

Technical Data

Features

Downloads: Centrifuge 5420