What do I have to consider for cleaning the Eppendorf µCuvette?

Always use for cleaning lint-free tissue papers. Do not use therefore any chemicals that are not recommended in the operation manual. Particularly substances that tend to corrosion such as acids or bases should be avoided. Nucleic acids could be removed easily with water. But it is also possible to clean the cuvette with a 6% sodium hypochlorite solution to remove persistent nucleic acid contaminations. A cleaning in a ultrasonic bath is not recommended

After adding the sample to the Eppendorf µCuvette no liquid column could be formed. What could be the reason for this?

One reason can be, that the volume of the used sample was simply too low. Another possibility is that after purification of DNA samples it may appear that there is still high amount of protein inside the sample. This may lead to a reduced surfaced tension of the liquid. This problem could be solved by slightly increasing the measuring volume. If solutions are containing detergent, it may appear that liquid column cannot be formed at all. In this case the sample should be measured in a cuvette (e.g. Eppendorf UVette).

In which spectrophotometer can the Eppendorf µCuvette be applied?

The Eppendorf µCuvette can be used for all Eppendorf BioSpectrometer and the BioPhotometer D30, 6131 und 6132. It can not be applied spectrophotometer of other manufacturers.

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Eppendorf µCuvette^® G1.0

Mechanism of Eppendorf _MICRO_Cuvette G1.0

Scientist filling the Eppendorf _MICRO_Cuvette G1.0 with a sample

Scientist at the bench is working with Eppendorf _MICRO_Cuvette G1.0

Scientist puts Eppendorf _MICRO_Cuvette G1.0 into photometer

Eppendorf BioPhotometer D30 with _MICRO_Cuvette

Eppendorf _MICRO_Cuvette G1.0 view from side

Product Information

One droplet is the limit?
The µCuvette G1.0 is in your range: Only 1.5 µL of a sample is needed. The optical path length of only 1 mm enables a light path that is 10x shorter than the light path on standard cuvettes: Measure high nucleic acid and protein concentrations.

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Products (1)

Accessories (2)

1 Products

Eppendorf µCuvette^® G1.0

Microvolume measuring cell for Eppendorf BioPhotometer^® and BioSpectrometer^®

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N.^o de catálogo 6138000018

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2.241,00 €

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Product Information

Technical Data

Features

The Eppendorf µCuvette G1.0 microvolume measuring cell is a high-quality cuvette made of aluminum and quartz glass. It is the perfect tool for measuring high concentrations in small volumes. With a fixed optical path length of only 1 mm, the µCuvette G1.0 features a light path that is ten times shorter than in standard cuvettes. This allows nucleic acid and protein concentrations to be measured with high reproducibility in a much higher concentration range without prior dilution.
Due to the hydrophobic coating on the quartz glass only 1.5 µL nucleic acid or 3 µL protein sample are required for precise formation of the liquid column. Self-absorption of the µCuvette G1.0 is very low, meaning that the entire measuring range of the photometer can be used. Furthermore, 5 µL of sample solution can be used for specific fluorometric assays saving reagent.
In general, the lower the concentration of a sample, the longer the optical path length should be. The same physical model (Beer´s Lambert Law) claims, if the concentration of a sample is very high, the light path length being used should be shortened to ensure enough light can pass through the sample to reach the detector. Microvolume measurements with light path lengths equal or shorter than 1mm are recommended for concentrations above 25 ng/µL dsDNA. If you are not sure which cuvette is the right solution for your sample, please visit our “About Detection” overview and check with the Cuvette Navigator. Please notice that the BioSpectrometers and BioPhotometers both accommodate standard cuvettes and the µCuvette G1.0. Thus, Eppendorf provides the flexibility of choosing the optimal light path length to detect a wide variety of sample concentrations!

Concentrations

Determining concentration via extinction factor and light path length is commonly performed in a cuvette format. In the case of standard cuvettes, the path length is determined by the width of the cuvette because the light beam travels through the cuvette horizontally. When using the µCuvette G1.0, the distance between the upper and the lower portion of the cuvette determine the path length. Thus, the measurements and the calculations can be performed identical to those in standard cuvettes. Once set in the parameters, the software automatically utilizes the right light path length when calculating concentrations.
A defined angle for opening and closing the cuvette allows for easy and reproducible handling. Furthermore, the hydrophobic surface coating on the quartz glass guarantees simple cleaning, precise sample positioning and stable formation of the liquid column.

Easy loading

The Eppendorf µCuvette G1.0 can easily filled by pipetting the sample solution into the circle.

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Características	Eppendorf µCuvette^® G1.0

Dimensions (W × D × H)	12,5 × 12,5 × 48 mm
Colorimetric protein assays	yes
Fluorescence measurements	yes
Light transmission	180 nm – 2,000 nm
Maximum filling volume	10 µL
Height of light source	8.5 mm
Use in Eppendorf BioPhotometer^®	yes
Use in Eppendorf BioSpectrometer^®	yes
OD 600 methods	no
Temperature control	no
Sample light path length (mm)	1 mm
Cuvette blank	≤0.05 A at 260 nm
Material	quartz glass (with hydrophobic coating)
dsDNA concentration range (UV 260 nm)	25 ng/µL – 1,500 ng/µL
BSA concentration range (UV 280 nm)	758 ng/µL – 45,450 ng/µL

Minimum filling volume
in Eppendorf photometers	1.5 µL

Descargas: Eppendorf µCuvette^® G1.0

Brochure

La chispa del futuro

Rendimiento "espectracular" – Equipos de fotometría Eppendorf y accesorios

Small sample volume, less effort: The new Eppendorf µCuvette™ G1.0

Pequeño volumen de muestra, menor esfuerzo:
La nueva Eppendorf µCuvette™ G1.0

PDF 1.6MB

Spanish

Brochure

Spark of the Future (Sales stop of Devices!)

Spectracular performance - Eppendorf photometer and accessories

PDF 2.2MB

English

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Eppendorf µCuvette® G1.0

µCuvette G1.0 mechanism

µCuvette G1.0

µCuvette G1.0, scientist filling

µCuvette G1.0 Scientist closing

µCuvette G1.0 in photometer

BioPhotometer D30 with µCuvette

µCuvette G1.0

Eppendorf BioSpectrometer fluorescence

µCuvette closed

Product Information

Concentrations

Easy loading

Technical Data

Features

Descargas: Eppendorf µCuvette® G1.0

Eppendorf µCuvette^® G1.0

Descargas: Eppendorf µCuvette^® G1.0