You’ve heard of 4D cinema, 4D digital printing, etc.… Now, gradients too come in dimensions?!
Now, we see another revolution in the gradient technology – the 2-dimensional or 2D-gradient. This technology enables the user to use two gradient steps in the same PCR. The original 1D-gradient gives 12 different temperatures horizontally across the block (some brand uses 8 temperatures across the rows from top to bottom strategy). With the new 2D-gradient, the thermal block can now additionally give 8 different temperatures vertically across the block. Both 2 gradients can be combined in one PCR program, thus allowing concurrent optimization of the denaturation step and the annealing step.