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Stem Cell Bioprocessing

Culture standardization and efficient scale-up

Stem cell-based technologies are one of the most promising approaches in the further advancement of cell therapy and regenerative medicine. Also, great hopes are linked to using stem cells as a tool for drug discovery. In order to make progress towards commercialization, researchers in the field are evaluating standardization of their cultivation and efficient scale-up.

Cultivation of cell aggregates

For many applications like disease modeling, drug toxicity assessment, and manufacture of stem cell-based products, three-dimensional (3D) cell aggregates are of great interest. Researchers at the Instituto de Biologia Experimental e Tecnológica (iBET) in Portugal have tested the suitability of the Eppendorf DASbox® Mini Bioreactor System for the cultivation of the human tumor cell line H157 as 3D cell aggregates.

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Controlled Cultivation of Stem Cells - Factors to Consider When Thinking of Scale-Up

Widely established in traditional cell culture, controlled bioreactors have the potential to establish reproducible expansion of stem cells. They provide extensive options for monitoring and control of key parameters such as pH and dissolved oxygen in real-time and facilitate a controlled differentiation of the cells. Through scalable bioreactor design, results obtained in small scale can be transferred to larger working volumes while maintaining optimum mass transfer.

Interview with Dr. Philipp Nold


Dr. Nold joined the Eppendorf Bioprocess Center as an Infield Application Specialist for stem cells. In this interview he speaks about the latest advances in bioprocessing.

Main aspect of this interview is: Biopharma know-how meets bioreactor innovation in upstream bioprocessing.

Click here to read the interview

Induced pluripotent stem cells


Since the pioneering development of cell reprogramming by Shinya Yamanaka in 2006, induced pluripotent stem cells (iPSCs) have risen to be the most promising alternative to ethically problematic embryonic stem cells. Controlled cultivation and scale-up in clinical grade as well as ways to control their differentiation towards their final destiny will be key steps towards commercial use of these cells.

Dr. Robert Zweigerdt and his team at Hannover Medical School are investigating human pluripotent stem cell culture for clinical use. They established a process using the Eppendorf DASbox® Mini Bioreactor System equipped with BioBLU® Single-Use Vessels. As part of the EU-funded research project TECHNOBEAT , Eppendorf and the Hannover Medical School aim at scaling up the culture and develop a GMP-compliant process.

Researchers from TissUse® GmbH generated hiPSC-derived neurospheres for drug research applications. They demonstrated the potential of the DASbox Mini Bioreactor for such a process.

A team of researchers from Ncardia® GmbH developed a bioprocess for the large-scale manufacturing of cardiomyocytes derived from a variety of healthy and diseased hiPSC lines. They expanded hiPSCs as cell aggregates in a DASbox® Mini Bioreactor System and scaled-up the process using a BioFlo 320 bioprocess control system.

Production of Human Induced Pluripotent Stem Cell- Derived Cortical Neurospheres in the DASbox ® Mini Bioreactor System

Researchers from TissUse® GmbH generated hiPSC-derived neurospheres for drug research applications. They demonstrated the potential of the DASbox Mini Bioreactor for such a process.

From stem cell production to hiPSC-based drug development: The flexibility of bioreactors

Stirred-tank bioreactors are gaining more and more importance in the industrial production of pharmaceutical products, biofuels and food.

Mesenchymal stem cells

Mesenchymal stem cells (MSCs) are multipotent cells derived from the bone marrow, from adipose tissue, cord blood or other tissues related to the embryonic mesoderm. They are relatively easy to obtain and have great potential for cell therapy and drug screening.

Various MSCs have been successfully cultivated in stirred-tank bioreactors.

Expansion of Human Bone Marrow-Derived Mesenchymal Stem Cells in BioBLU® 0.3c Single-Use Bioreactors

The use of adult stem cells holds great promise for new cell-based therapies and drug discovery.

Billion-Cell Hypoxic Expansion

Stem cell-based regenerative medicine has great potential to advance the therapeutic treatment of human diseases.

Process development and optimization using packed bed bioreactor systems for ensuring successful launch into large scale manufacturing

Researchers at Pluristem® have gained a lot of experiences with packed-bed bioreactors and are using this technology for stem cell bioprocessing. Biomass derived in that way has been used in a Phase 1 clinical trial.

Stem Cell Community Day

The Stem Cell Community Day connects researchers from all over the world to review recent advances in the area of stem cells, with a special focus on cultivation in stirred-tank bioreactors.

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At our 1st Stem Cell Community Day in April 2017 we brought together experts from industry and academia, to discuss latest achievements, challenges, and chances in stem cell bioprocessing for research and commercial manufacturing.

eBook: Advancing medicine - Can cell and gene therapies paint a picture of perfect future health?
(The Medicine Maker, sponsored by Eppendorf)
3.2 MB, PDF

Download eBook

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