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Eppendorf BioSpectrometer® kinetic
BioSpectrometer kinetic front
Eppendorf BioSpectrometer® kinetic: Convenient user interface for easy handling
BioSpectrometer kinetic front
Eppendorf BioSpectrometer® kinetic: Convenient user interface for easy handling
BioSpectrometer basic and kinetic
BioSpectrometer kinetic detail
Eppendorf detection instruments: Protect your sample during the measurement
Spectrometer measurements of hexokinase UserGuide 039
Eppendorf detection instrument software: Hexokinase measurement
µCuvette G1.0 closed
µCuvette G1.0 mechanism
UVette light paths
VIS Cuvette macro
VIS Cuvette semi-micro
University of East London
Product Information
Measuring live enzymatic reactions?The Eppendorf BioSpectrometer kinetic offers a temperature-controlled cuvette shaft for time-curve experiments. As member of the Eppendorf Spectrometer family, it combines easy handling with highly reproducible analysis. A variety of cuvettes expands the options for this detection instrument even further from microvolume to macrovolume measurements.
You will find additional download material at the bottom of this page
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Technical Data
Applications
Features
The Eppendorf BioSpectrometer kinetic is an expansion of the Eppendorf BioSpectrometer basic and offers preprogrammed and freely programmable timed and temperature controlled assays. It features an integrated heated cuvette shaft. Its integrated peltier element guarantees high-precision for determining enzyme and substrate kinetics as well as other temperature-sensitive applications. Guided software is included; minimal effort is required to set up new assays. Retrospective modification of the time window for regression analysis can be made directly on the device. The BioSpectrometer kinetic replaces the traditional large cumbersome setup required for temperature controlled timed assays.
Measuring live enzymatic reactions?
The Eppendorf BioSpectrometer kinetic offers a temperature-controlled cuvette shaft for time-curve experiments. The integrated Peltier element guarantees high-precision temperature control.
Annoyed by uncomfortable handling?
Ergonomics is far beyond the »ergonomically designed chair«. Eppendorf started to optimize the laboratory devices regarding ergonomics already in the early 1970s.
In 2003, we started the PhysioCare Concept, focusing on ergonomic liquid handling devices like our pipettes.
Nowadays, the Eppendorf PhysioCare Concept is broaden up to include further laboratory products as a holistic solution to harmonize the workflow in your laboratory with your health and well-being, e.g. for the Eppendorf BioSpectrometer:
• Guided software for easy handling
• Clear indications at cuvette shaft for convenient handling
• Direct keys for convenient handling
• Small footprint to enable position in direct arm reach at your bench
• Standardized »user-interface concept« for intuitive handling of devices
Further information about the Eppendorf PhysioCare Concept:
www.eppendorf.com/physiocare/
Struggling with paper-based documentation?
The eLABJournal Electronic Lab Notebook offers an intuitive and flexible solution to document research and track research data like BioSpectrometer–based values. Improve efficiency when documenting, organizing, searching, and archiving collected data. With the free eLABJournal add-ons, you can extend the functionality of eLABJournal to a fully integrated Laboratory Information Management System.
Start a free 30-day trial!
Visit www.eLABJournal.com
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| Specifications | Eppendorf BioSpectrometer® kinetic |
|---|
| Interfaces |
• USB master: for USB stick and thermal printer DPU-S445 • USB slave for connecting to a PC (All functions available without PC) • Serial interface RS-232: for thermal printer DPU-414 • Ethernet interface RJ45: For connecting to a network printer or emailing data outputs directly off instrument |
| Power supply | 100 – 240 V ±10 %, 50 – 60 Hz |
| Dimensions (W × D × H) | 29.5 × 40.0 × 15.0 cm / 11.6 × 15.7 × 6 in |
| Weight w/o accessories | 5.5 kg / 12.1 lb |
| Interval between two measuring points | 00:05 – 10:00 min:sec |
| Measuring time range | 00:05 – 59:59 min:sec |
| Adjustable temperature range | 20 – 42 °C (smallest step size 0.1 °C) |
| Random error wavelength | ≤0.5 nm |
| Systematic error absorption | ±1 % (A = 1) |
| dsDNA concentration range (for 1 to 10 mm path length) | 2.5 ng/µL – 1,500 ng/µL |
| BSA concentration range (UV 280 nm) µCuvette® G1.0 | 758 ng/µL – 45,450 ng/µL |
| dsDNA concentration range (UV 260 nm) µCuvette® G1.0 | 25 ng/µL – 1,500 ng/µL |
| dsDNA concentration range (UV 260 nm) UVette® 2 mm | 12.5 ng/µL – 750 ng/µL |
| dsDNA concentration range (UV 260 nm) UVette® 10 mm | 2.5 ng/µL – 150 ng/µL |
| BSA concentration range (UV 280 nm) UVette® 2 mm | 379 ng/µL – 22,725 ng/µL |
| BSA concentration range (UV 280 nm) UVette® 10 mm | 76 ng/µL – 4,545 ng/µL |
| BSA concentration range (for 1 to 10 mm path length, calculation factor for BSA = 1.515 µg/µL) | 76 ng/µL – 45,450 ng/µL |
| Wavelength range absorption | Scan (nm): 200 – 830 at 1 nm increments |
| Wavelength Selection Increment | 1 nm |
| Scanning | yes |
| Wavelength range | 200 nm – 830 nm |
| Sample light path length (mm) | 8.5 mm |
| Measuring principle absorption | single-beam absorption spectrophotometer with reference beam |
| Light source absorption | Xenon flash lamp |
| Spectral bandwidth (mm) | ≤4 nm |
| Smallest step size | 1 nm |
| Absorption measuring range | 0 A – 3.0 A (260 nm) |
| Random error absorption |
≤0.002 at A = 0 ≤0.005 (0.5 %) at A = 1 |
| Systematic error wavelength | ±1 nm |
| Cuvette shaft | 12.5 mm × 12.5 mm, tempered 20 °C – 42 °C |
| Detector Type | CMOS photodiode array, 1024 pixel |
| Power consumption |
Approx. 30 W during operating step Approx. 5 W with dimmed display and temperature control switched off |
| Operator guidance language | Spanish, Italian, French, English, German, Japanese |
| Method memory | >100 method programs |
- All Eppendorf BioSpectrometer basic applications
- Additional kinetic applications, and enzyme activity testings at different temperatures e.g.:
Hexokinase activity measurements (340 nm, 37 °C)
Determination of ATP concentration (340 nm, 37 °C)
Beta-galactosidase assays (420 nm, 30 °C)
- All Eppendorf BioSpectrometer basic options
- Integrated temperature-controlled cuvette shaft (adjustment range +20 °C to +42 °C in 0.1 °C increments)
- Integrated interval timer (minimum interval 5 s, maximum measuring time range 59 min 59 s)
- Preprogrammed and freely programmable kinetic methods
- Retrospective modification of the time window for regression analysis
- Data transfer via USB interface, Ethernet or via e-mail and direct printout of results
- Data output as *.PNG screenshots, Microsoft® Excel®, or secure PDF files to a standard USB flash drive or via email
Downloads: Eppendorf BioSpectrometer® kinetic
Eppendorf SE
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