Are Eppendorf products free of RNA?

All products certified with "PCR clean" are tested free of human DNA, DNase, RNase and PCR inhibitors. The following applies from our point of view: Where no human DNA is detected, there can also be no RNA, which is considerably more unstable.

What is the meaning of the PCR Clean seal?

For molecular-biological requirements Eppendorf offers a new standard for uniform purity specifications. All consumables marked with the "Certified PCR Clean” label are guaranteed free of human DNA, DNase, RNase and PCR inhibitors.

Why are there double labels on the folding boxes, as "PCR clean" + "Sterile" or "PCR clean" + "Protein-free"?

Due to the modularization of purity grades, some products are labeled with more than one purity grade symbol. For example, the epDualfilter T.I.P.S. has the double seal "PCR clean "+ "Sterile" and the UVette the double seal "PCR clean" + "Protein-free" on the packing. The certification remains unaffected by the modularization.

Are lot specific certificates for the purity grades available in the internet?

Yes, the lot specific purity certificates, so-called Test report, can be downloaded at the certificates homepage .

Can products of the previous and new modular purity grades (with new seals) be used simultaneously in one application?

Yes, if no batch attachment is required for the application, products of the old and new modular purity grades can be used, as their specifications are the same.

Which advantages do Eppendorf Polypropylene Microplates have over plates made from polystyrene?

Plates made from polypropylene may be used across a broad spectrum of applications, as they are more resistant to temperature variations as well as various chemicals than plates made from polystyrene. Furthermore, they are more robust, allowing them to be centrifuged at higher g-forces. In addition, biomolecules, such as nucleic acids and proteins, bind less strongly to polypropylene.

Are Eppendorf PP Microplates resistant to organic solvents, e.g. DMSO?

The Eppendorf PP Microplates are made from polypropylene and are thus resistant to DMSO and other organic solvents, unlike plates made from polystyrene. An overview of chemical resistance can be found in Application Note no. 56.

Are the colored rim and labeling resistant to organic solvents?

All areas of the plate, including the colored rim, are resistant to chemicals. The alpha numeric labeling is equally durable. An overview of chemical resistance can be found in Application Note no. 56.

How may the Eppendorf PP Microplates be sealed?

Eppendorf offers a variety of sealing options for Microplates. Self-adhesive sealers, films and foils for heat sealing, as well as re-usable sealing mats, are available. Information regarding the compatibility of the different sealing methods is listed in the Application Note no. 208.

Which advantages do the black Eppendorf PP Microplates offer?

Plates made from black material are especially well suited for fluorescence assays (e.g. PicoGreen ® ). The black wells prevent “cross talk” between wells and reduce background signals. The black Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in black plates. Due to carefully selected materials, the plates have very low autofluorescence. The superior signal-to-noise ratio contributes to the increased sensitivity of fluorescence assays (see Application Note no. 203).

Which advantages do the white Eppendorf PP Microplates offer?

Plates made from white material may be used for luminescence assays (chemiluminescence, scintillation proximity assays (SPA) etc.), as well as for fluorescence assays (fluorescence intensity, polarization, etc.). The white wells prevent “cross talk” between the wells while amplifying the individual signals. The white Eppendorf PP Microplates feature the unique OptiTrack Matrix, the first technology to allow for fast and easy well identification in white plates. Due to carefully selected materials, the plates offer maximum signal amplification. Due to their very low autofluorescence, the white Eppendorf Microplates are equally well suited for fluorescence assays. Taken together, these features allow for an improvement in the sensitivity of analyses.

May the Eppendorf PP Microplates be stored at –196°C in liquid nitrogen?

No, we recommend storage at a minimum of –80°C.

May the Eppendorf PP Microplates be autoclaved?

Yes; however, the exact specifications of the Microplates could be compromised. The plates are available in the qualities sterile or PCR clean. Hence, autoclaving is necessary only under exceptional circumstances.

Are the Eppendorf PP Microplates in LoBind quality coated or siliconized?

No, the LoBind quality of the Eppendorf PP Microplates is not derived from siliconization or any other type of coating. The functionality is achieved through a carefully selected material, in combination with a special manufacturing process.

How high may Eppendorf PP Microplates be centrifuged?

The Eppendorf PP Microplates may be centrifuged up to 6000 x g. Please follow the instructions provided in the manual.

May immunoassays such as ELISA be performed in the Eppendorf PP Microplates?

Plates made from polypropylene are not suited for the standard variant, where antigen or antibodies are bound to the well wall. For this application, plates made from polystyrene are used, which have a higher binding capacity for biomolecules. However, for antibody-based fluorescence or luminescence assays (e.g. with beads) Eppendorf PP Microplates with black or white wells are suitable, respectively.

Is it possible to perform absorption measurements in the Eppendorf PP Microplates?

Typically, absorption measurements are not performed in plates made from polypropylene, but in plates made from polystyrene, as this material generally has a very high transparency. Since the Eppendorf PP Microplates show considerably higher transparency compared to other plates made from polypropylene (see Application Note no. 202), the Eppendorf Microplates may be used for absorption measurements in certain cases.

Why are the Eppendorf PP Microplates offered in Sterile and PCR clean, but not in Eppendorf quality?

The Eppendorf PP Microplates are a very high quality product. In order to underline the quality, all plates are available in the degrees of purity PCR clean or Sterile. This increases the confidence and comfort of the user, who may employ the plates directly for valuable samples in sophisticated applications.

Are Eppendorf PP Microplates also available with barcode?

Yes, the option of Eppendorf Microplates with barcode is available. Further information can be found on the product page microplates .

Are Eppendorf tubes and plates (also LoBind variants) resistant to 100% acetonitrile?

Eppendorf tubes and plates including LoBind variants are resistant against 100% acetonitrile only in room temperature conditions. Above 40°C and prolonged exposure (days) may lead to partial damage and is not recommended. For lower acetonitrile concentrations the resistance parameters will be better so prolonged exposure and higher temperatures may be possible. This must be however tested by the customer.

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Eppendorf Microplates

Eppendorf Microplate with OptiTrack® matrix

Selection of microplates stacked on top of each other

Four microplates, 384-well black and transparent plate, 96-well white and transparent plate

Sample being pipetted into a microplate with Combitip® pipette tips

Eppendorf Microplate in an automated liquid handling instrument

Woman in lab coat looking through microplate

Barcode Option Available

Experience a new level of transparency with Eppendorf Microplates! With the highest quality polypropylene and superior well design, you can pipette, control and recover samples with minimal effort. Plus, we offer a plate for every application.

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Pipette, control and recover your samples with ease using Eppendorf Microplates! Designed with the easy-to-read alphanumeric OptiTrack^® matrix label to identify individual wells, speed up the filling process and significantly reduce the risk of pipetting errors. Eppendorf Microplates are made with high-transparency polypropylene ensuring quick and easy location of samples and are also available in Eppendorf LoBind^® for when you need maximum sample recovery.

Superior transparency and OptiTrack^® matrix

Rest assured that our Eppendorf Microplates are manufactured from the highest quality polypropylene to ensure maximum transparency and improve reproducibility. Let the contrast-rich OptiTrack matrix guide you to lab efficiency – identify wells in record time and reduce the risk of error and contamination.
Learn more about how Eppendorf Microplates can help rapidly identify samples by reading Application Note 202: Eppendorf Polypropylene Microplates – Fast and secure identification of samples.

Maximum recovery in minimal time with RecoverMax^® well design

Get Maximal sample recovery and superior mixing properties with Eppendorf microplate’s RecoverMax^® optimized well geometry. A round and smooth design of well corners helps to prevent sample loss and minimize residual volume while the smooth contours of square-well plates virtually eradicate the risk of cross contamination.

Eppendorf Microplates offer the ultimate transparency and quality in a variety of formats:

• 96 and 384 well formats
• F–bottom (flat), U–bottom (round), and V–bottom (conical).
• Eppendorf PCR clean and Sterile purity grades.
• Also available as DNA and Protein LoBind.
• g–Safe^®: exceptional centrifugation stability up to 6,000 x g for faster protocols and improved sample quality.
• Raised well rims and smooth surface for reliable closing, even with heat sealing
• High–quality polypropylene guarantees resistance to chemicals, mechanical stress and temperature extremes.
• Autoclavable (121 °C, 20 min).
• Manufactured without demoulding aids, softeners or biocides

For more information see Application Note 56: The best material for original Eppendorf Tubes^® and Plates: Properties and chemical resistance of polypropylene. Application Note 239: Eppendorf Deepwell Plates and Microplates - Investigations of sealing methods.